Virus load and virus shedding of SARS-CoV-2 and their impact on patient outcomes.

Background: Understanding a virus shedding patterns in body fluids/secretions is important to determine the samples to be used for diagnosis and to formulate infection control measures.

Aim: To investigate the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) shedding patterns and its risk factors.

Methods: All laboratory-confirmed coronavirus disease 2019 patients with complete medical records admitted to the Shenzhen Third People's Hospital from January 28, 2020 to March 8, 2020 were included.

Enhancement of A82846B yield and proportion by overexpressing the halogenase gene in Amycolatopsis orientalis SIPI18099.

The glycopeptide antibiotic A82846B (chloroeremomycin) produced by Amycolatopsis orientalis is the precursor of the semi-synthetic antibiotic oritavancin. However, during the industrial production of A82846B, two major impurities, A82846A (63.6%) and A82846C (12%) which are structurally similar to A82846B (24.

Congenital Hyperinsulinism in China: A Review of Chinese Literature Over the Past 15 Years.

Objective: Congenital hyperinsulinism (CHI) is a rare but severe cause of hypoglycemia. The present study investigates the clinical presentation, therapeutic outcomes and genetic mutations of CHI in Chinese individuals over the past 15 years.

Methods: The authors retrospectively reviewed one case in their department and 206 cases reported from January 2002 to October 2016 in China.

[Extraction Method of Malaria Parasite DNA from Preserved Positive Blood Smears].

Objective: To develop a method for DNA extraction from malaria parasites on preserved blood smears, to provide basis for research on malaria genetic traceability.

Methods: The improved DNA extraction kit (QIAamp DNA Mini Kit) was used to extract plasmodium DNA from 41 giemsa-stained blood smears, and the extraction was compared with that using the Chelex-100 and Na(2)HPO(4) methods. Nested PCR was used to amplify small subunit ribosomal RNA to identify Plasmodium parasite.

[Cloning, expression, purification and identification of Toxoplasma gondii SAG2 gene in Escherichia coli].

Objective: To construct a recombinant plasmid containing surface antigen 2(SAG2) gene of Toxoplasma gondii and express it in Escherichia coli.

Methods: The truncated SAG2 gene was amplified from the genomic DNA of T. gondii RH strain and cloned into plasmid pGEX-4T.

SIRT3 inhibits cell proliferation in human gastric cancer through down-regulation of Notch-1.

SIRT3 is a member of the NAD+-dependent class III deacetylase sirtuin family and plays pivotal roles in regulating cellular functions. Accumulating evidence has recently demonstrated that SIRT3 may function as either oncogene or tumor suppressor in a panel of cancers. However, the biological function of SIRT3 in gastric cancer has been poorly characterized.

[Application of Nested PCR in the Diagnosis of Imported Plasmodium Ovale Infection].

Objective: To identity Plasmodium ovale infection by 18S rRNA gene nested PCR.

Methods: Whole blood and filter paper blood samples of malaria patients in Shandong Province were collected during 2012-2013. The parasites were observed under a microscope with Giemsa staining.

Pyrosequencing reveals fungal communities in the rhizosphere of Xinjiang Jujube.

Fungi are important soil components as both decomposers and plant symbionts and play a major role in ecological and biogeochemical processes. However, little is known about the richness and structure of fungal communities. DNA sequencing technologies allow for the direct estimation of microbial community diversity, avoiding culture-based biases.

Enterovirus infections are associated with white matter damage in neonates.

Aim: To explore the imaging findings of neonatal infants infected with enteroviruses.

Methods: A retrospective study was conducted on 12 patients who were diagnosed with encephalitis caused by enterovirus. Clinical presentation, cranial ultrasonography (cUS), magnetic resonance imaging (MRI) findings and neurodevelopment outcome of 12 cases were analysed.

Ni3S2/Ni-P bilayer coated on polyimide as a Pt- and TCO-free flexible counter electrode for dye-sensitized solar cells.

In this study, we reported an efficient, flexible, and low-cost (Pt-free and transparent conducting oxide (TCO)-free) counter electrode (CE) made of a polyimide (PI) substrate coated with a Ni3S2/Ni-P bilayer for dye-sensitized solar cells (DSCs). The bilayer Ni3S2/Ni-P hybrid film was deposited on a PI plastic substrate via a series of wet chemical/electrochemical processes. The bottom Ni-P layer was deposited on a PI to replace conventional TCO as a conductive layer, and the top Ni3S2 layer was employed as the electrocatalyst for I3(-) reduction.

[Clinical observation on abdominal electroacupuncture for treatment of poststroke constipation].

Objective: To compare therapeutic effects of abdominal electroacupuncture (EA) and western medicine on poststroke constipation.

Methods: Eighty cases were randomly divided into an EA group and a medication group, 40 cases in each group. The EA group were treated with EA at Daheng (SP 15), Fujie (SP 14), Tianshu (ST 25), Shuidao (ST 28), etc.