Hfq-Antisense RNA I Binding Regulates RNase E-Dependent RNA Stability and ColE1 Plasmid Copy Number.

The mechanisms and consequences of gene regulation by Hfq on -encoded small RNAs (sRNAs) have been well studied and documented. Recent employment of Genomic SELEX to search for Hfq-binding motifs has indicated that Hfq might frequently regulate gene expression controlled by -antisense RNAs. Here, we use the classic ColE1 plasmid antisense RNA-based regulation model (i.

Combined Transcriptomic and Proteomic Profiling of under Microaerobic versus Aerobic Conditions: The Multifaceted Roles of Noncoding Small RNAs and Oxygen-Dependent Sensing in Global Gene Expression Control.

Adaptive mechanisms that facilitate intestinal colonization by the human microbiota, including , may be better understood by analyzing the physiology and gene expression of bacteria in low-oxygen environments. We used high-throughput transcriptomics and proteomics to compare the expression profiles of grown under aerobic versus microaerobic conditions. Clustering of high-abundance transcripts under microaerobiosis highlighted genes controlling acid-stress adaptation (, , and operons), cell adhesion/biofilm formation ( and operons), electron transport (), oligopeptide transport (), and anaerobic respiration/fermentation ( and operons).