Heart Rate Response Predicts 6-Minutes Walking Distance in Pulmonary Arterial Hypertension.

Because the 6-minute walking test (6MWT) is a self-paced submaximal test, the 6-minute walking distance (6MWD) is substantially influenced by individual effort level and physical condition, which is difficult to quantify. We aimed to explore the optimal indicator reflecting the perceived effort level during 6MWT. We prospectively enrolled 76 patients with pulmonary arterial hypertension and 152 healthy participants; they performed 2 6MWTs at 2 different speeds: (1) at leisurely speed, as performed in daily life without extra effort (leisure 6MWT) and (2) an increased walking speed, walking as the guideline indicated (standard 6MWT).

HeLa-CCL2 cell heterogeneity studied by single-cell DNA and RNA sequencing.

The HeLa cells are the earliest and mostly used laboratory human cells for biomedical particularly cancer research. They were derived from a patient's cervical cancerous tissue, and are known for their heterogeneous cellular origin and variable genomic landscapes. Single-cell sequencing techniques with faithful linear and uniformly amplified genomes (DNA) and transcriptomes (RNA) may facilitate the study of cellular differences at the individual cell level.

[Methods of BMP immobilization and evaluation for Ti-based dental implant surface modification].

Ti-based biomaterial has been widely used as dental and bone implant material although its bioactivity still needs improvement especially for dental implant. The bone morphogenetic proteins(BMPs) bound to Ti-based materials will attract the mesenchymal stem cells to differentiate into osteoblast cells, which benefits the response to the protein-material surface, and finally leads to new bone formation. Several methods including physically mixing, coating, plasma immobilization and cross-linking were used to investigate how BMPs bind to Ti-based biomaterials.

Clinical investigation for bilateral cleft lip repair: modified functional bilateral cleft lip cheilorrhaphy.

Purpose: To obtain better operative results, a modified functional bilateral cleft lip (BCL) cheilorrhaphy was designed and used on 131 BCL patients to evaluate the clinical effectiveness in this clinical investigation. A new surgical method would be provided for BCL patients.

Patients And Methods: Based on the experiences and advantages of the commonly used self-longation and elongation surgical methods for BCL repair, a new surgical method was designed for BCL patients.

Protein preparation and preliminary X-ray crystallographic analysis of a putative glucosamine 6-phosphate deaminase from Streptococcus mutants.

The SMU.636 protein from Streptococcus mutans is a putative glucosamine 6-phosphate deaminase with 233 residues. The smu.

Surgical repair of hard palate cleft with absorbable membrane: the new surgical technique and its clinical application.

This study evaluated a new surgical technique with absorbable membrane to repair hard palate cleft without extensive mobilisation of the mucoperiosteum. From 2001 to 2002, 32 selected patients with complete unilateral clefts underwent this surgical operation. The traditional flap surgical operation was performed at the soft palate, uvula and anterior alveolar cleft.

[Ultrastructure of poly-DL-lactic acid as GTR membrane of soft tissue defects in goats palates].

Objective: To study the ultrastructure changes of PDLLA as GTR membrane of soft tissue defects in the palates of goats.

Methods: To establish penetrating rectangle palate defects in 8 goats. PDLLA membranes were implanted into the defect areas to guide the regeneration of the soft tissue defects.

[Study on chronic toxicity to rats of implantation of super-high molecular weight polylactate].

Objective: To make sure whether super-high molecular weight polylactate is toxic to the body after it has been implanted into the body for a long period.

Methods: We implanted super-high molecular weight polylactate into the rats and took the specimens of blood at 3, 6, 9, 12 months after the operation. The changes of proteins, electrolyte, enzyme and other indices were observed.

[Guided reconstruction of soft tissue defects of the palate with n-HA/PA66 plates: an experimental study in goats.].

Purpose: To evaluate the effect of n-HA/PA66 plate as a guided implant for the regeneration of the soft tissue defects in palatal region of goats.

Methods: 12 goats were divided into experimental group and control group at random. 18.

[Repair of hard cleft palate with absorbable membranes made by poly-DL-lactic acid: a feasibility study].

Objective: To investigate the feasibility and clinical results of applying poly-DL-lactic acid (PDLLA) biomembranes in cleft palate repair.

Methods: 68 cleft palate patients were divided into study group and control group. The traditional surgical method was used to control group to close the soft cleft palate, and the PDLLA biomembrane was used to study group and implanted into the surgical gap between the periosteum and bone at the hard palate, and fixed with suture.

[Experimental study on the reconstruction of mandibular defects with a new bioactive artificial bone nano-hydroxyapatite/polyamide-66 in dogs].

Objective: This study was designed to investigate reconstruction of segmental defect in the mandible using a new bionic materials of nano-hydroxyapatite -polyainides-66 (n-HA/PA66).

Methods: Two defects (15 mm x 10 mm x 5 mm) were created in the mandibular bodies of dogs. One of defects was reconstructed with n-HA/PA66, another not repaired as a blank control.

[Influence of super high molecular weight poly D,L-lactic acid on viability and new bone formation of osteoblasts].

Objective: To investigate the influence of the viability and new bone formation of osteoblasts by the super high molecular weight poly D,L-lactic acid (SHMW-PDLLA).

Methods: 1. The osteoblasts derived from neonatal rat were grown and maintained at steep of SHMW-PDLLA and normal culture medium.