Effects of Castration Age on the Growth Performance of Nubian Crossbred Male Goats.

To determine the optimal timing for performing castration on goats, eighteen male Nubian crossbred goats were randomly assigned to two groups and castrated at 3 months and 6 months of age, respectively. Daily dry matter intake, biweekly body weights, and ultrasonic measurements of longissimus dorsi muscle growth were recorded. Results indicated that there was no significant difference between the two groups in terms of the blood parameter analysis (except testosterone, 0.

Demethylation of the promoter suppresses migration and invasion in breast cancer.

miR-145 has been implicated in the progression of breast cancer. Here, we report that its expression is decreased in breast cancer specimens and cell lines and that this low level of expression is associated with DNA methylation of its gene, Methylation of has previously been correlated with cell migration and invasion, both and . We found that demethylation of reactivates miR-145 and contributes to the anti-cancer properties of 5-aza-2'-deoxyazacytidine (5-AzaC).

Serum deprivation elevates the levels of microvesicles with different size distributions and selectively enriched proteins in human myeloma cells in vitro.

Aim: To investigate the effects of serum deprivation (SD) on microvesicles (MVs) secreted from human myeloma cells and the implications for disease progression.

Methods: RPMI 8226, U266, and KM3 human myeloma cells were incubated in medium containing 10% (non-SD) or 1% fetal bovine serum (SD) and MVs were isolated. The levels and size distribution of MVs were analyzed with flow cytometry.

Angiogenesis-related cytokines in serum of proliferative diabetic retinopathy patients before and after vitrectomy.

Aim: To evaluate serum concentrations of angiogenesis-related cytokines in proliferative diabetic retinopathy (PDR) before and after vitrectomy.

Methods: Serum samples were collected from 30 PDR patients with varying severity before and after vitrectomy. Serum concentrations of vascular endothelial growth factor (VEGF), pigment epithelium-derived factor (PEDF), interleukin-8 (IL-8) and interferon-inducible protein-10 (IP-10) were determined by enzyme-linked immunosorbent assays (ELISA).

The analysis of injured and poisoned patients during the construction of urbanization and industrialization from 1998 to 2002.

Objective: To analyze the causes of injury and poisoning during construction of urbanization and industrialization and to explore proper measures.

Methods: The data of the patients with injury and poisoning during construction of urbanization and industrialization treated in out hospital from 1998 to 2002 were retrospectively analyzed.

Results: The number of discharged patients of injury and poisoning accounted for 29.

Clinical analysis of craniocerebral trauma complicated with thoracoabdominal injuries in 2165 cases.

Objective: To explore the optimal treatment for craniocerebral trauma complicated with thoraco-abdominal injuries.

Methods: A total of 2165 cases of craniocerebral trauma complicated with thoraco-abdominal injuries admitted to our hospital between July 1993 and June 2003 were retrospectively studied. Among them, 382 cases sustained severe craniocerebral trauma (in which 167 were complicated with shock), 733 thoracic injuries, 645 abdominal injuries and 787 thoraco-abdominal injuries.

[Changes and distribution of ATPase activity in the paranodules of Nicotiana tabacum].

Lead phosphate deposition technique was used to investigate the changes and characteristic distribution of ATPase activity in the paranodules of tabacoo. We found that ATPase activity was closely associated with cell type and cell developmental state, thus it was different in various cells and rhizobia. No lead phosphate granules were located in the menstematic cells, although there were a small number of lead phosphate granules in the cytoplasm and organelles of the cells without rhizobia, they did not exist in the young and mature rhizobia.

Antagonism of Paenibacillus lentimorbus to Botryosphaeria dothidea and Biological Control of Panicle and Shoot Blight of Pistachio.

A potential microbial fungicide, Paenibacillus lentimorbus isolate CBCA-2, against Botryosphaeria dothidea, the pistachio panicle and shoot blight fungus, was obtained from healthy pistachio leaves by both in vitro and in vivo screening techniques. CBCA-2 caused 100% inhibition of pycnidiospore germination after 24 h incubation at 25°C. Malformation of pycnidiospores and hyphae, and lysis and swollen pycnidiospores of B.

[Quantitation of telomerase activity using the polymerase chain reaction-based enzyme immunoassay and its clinical application].

Objective: To investigate detective methodology of telomerase activity and its clinical application.

Methods: The telomerase activity was quantitated by polymerase chain reaction-base enzyme immunoassay (PCR-EIA). The TS primer was biotinylated (TS-B), and CX primer was digoxigeninated (CX-D).

Genetic Variation Among Fusarium oxysporum Isolates Associated with Root Rot of Amaranthus hybridus in South Africa.

Thirty isolates of Fusarium oxysporum were recovered from six substrates: (i) tap roots of Amaranthus hybridus showing symptoms of root rot, (ii) side roots of A. hybridus showing symptoms of root rot, (iii) soil surrounding plants of A. hybridus, (iv) pigweed weevils (Hypolixus haerens) that feed on A.