[Isolation, culture and biological characterization of Beagle stem cells from apical papilla].

Objective: To isolate and characterize the Beagle stem cells from apical papilla.

Methods: Apical papilla was severed from the end of freshly extracted Beagle's young permanent upper anterior teeth, and digested by collagenase type I and dispase for cell culture. The isolated cells were investigated for stem cell properties by analyzing their colony-forming efficiency, growth characteristics and the expression of mesenchymal stem cell markers; and evaluating their multidifferentiation potentials including osteogenic, adipogenic, and chondrogenic potentials.

Effect of skimmed pasteurized milk and Hank's balanced salt solution on viability and osteogenic differentiation of human periodontal ligament stem cells.

Background/aim: The purpose of this study was to compare the effect of skimmed pasteurized milk and Hank's balanced salt solution on the viability and osteogenic differentiation potential of the human periodontal ligament stem cells at room temperature in vitro.

Material And Methods: Human periodontal ligament stem cells were obtained from extracted healthy third molars and conserved in skimmed pasteurized milk and Hank's balanced salt solution for 1, 2, and 4 h at room temperature to detect the viability of the cells and their osteogenic differentiation potential.

Results: The efficacy of skimmed pasteurized milk on cell viability at 4 h was significantly higher than that of HBSS (P < 0.