Tumor necrosis factor-alpha gene from mandarin fish, Siniperca chuatsi: molecular cloning, cytotoxicity analysis and expression profile.

In this report, we cloned a cDNA and the gene of mandarin fish (Siniperca chuatsi) tumor necrosis factor-alpha (TNF-alpha). The length of TNF-alpha cDNA was 1461 bp, contained an open reading frame (ORF) of 762 bp, which encoded 253 amino acids. The positions of cysteine residues, transmembrane sequence, and protease cleavage site were similar with other reported fish TNF-alpha and mammalian TNF-alpha.

Differential gene expression profile in spleen of mandarin fish Siniperca chuatsi infected with ISKNV, derived from suppression subtractive hybridization.

To study the interaction between an invading virus and its host, we investigated differential gene expression in mandarin fish Siniperca chuatsi experimentally infected with infectious spleen and kidney necrosis virus (ISKNV). Subtractive cDNA libraries were constructed by suppression subtractive hybridization (SSH) from spleens of mock- and ISKNV-infected fish. Both forward- and reverse-subtracted libraries were generated.