[Ion Torrent PGM sequencing for detection of Y chromosome microdeletion in 87 cases of azoospermia].

Objective: To explore the feasibility of Ion Torrent PGM sequencing in detection of Y chromosome microdeletion.

Methods: We enrolled 87 infertility patients with non-obstructive azoospermia (NOA) in this study and analyzed their routine semen parameters, reproductive hormone levels and chromosomal karyotypes. We detected Y chromosome microdeletion in the patients by Ion Torrent PGM sequencing and multiplex PCR, and compared the detection rates between the two methods.

Partial (two-thirds) nephrectomy in pigs: A comparison of three surgical approaches.

Partial (2/3) nephrectomy can be performed via the laparoscopic, retroperitoneal, and transperitoneal approach. Outcomes of the three approaches were compared in this study. 2/3 nephrectomy were performed in 21 healthy Bama miniature pigs (mean bodyweight 20.

[Disorder of copper homeostasis induced by lead exposure among mice and intervention effect of quercetin].

Objective: To investigate the effect of lead exposure on copper and copper metalloenzyme and the intervention effect of quercetin.

Methods: Twenty-four specific pathogen-free male Sprague-Dawley rats of good health were randomly divided into control group (n = 8), lead acetate group (n = 8), and lead acetate + quercetin group (n = 8). The rats in lead acetate group were poisoned by drinking water with 1 g/L lead acetate for 8 weeks, while the rats in control group were fed by drinking water with sodium acetate of the same volume for 8 weeks; the rats in lead acetate+quercetin group were intraperitoneally injected with quercetin (30 mg × kg-1 × d-1) for 8 weeks while drinking water with lead acetate.