Maternal inappropriate calcium intake aggravates dietary-induced obesity in male offspring by affecting the differentiation potential of mesenchymal stem cells.

Background: The effects of inappropriate dietary calcium intake in early life on later obesity have not been fully elucidated.

Aim: To raise the mechanism of maternal calcium intake on the multi-differentiation potential of mesenchymal stem cells among their male offspring.

Methods: Four-week-old female C57BL/6N mice were fed by deficient, low, normal and excessive calcium reproductive diets throughout pregnancy and lactation.

Incidence of and risk factors for surgical site infection after colorectal surgery: A multiple-center prospective study of 3,663 consecutive patients in China.

Background: Surgical site infection (SSI) after colorectal surgery (CRS) remains a significant problem for its negative clinical outcomes. However, it is poorly understood in China. This study aims to investigate the incidence, risk factors and microbiology of SSI after CRS.

[Indicators of Groundwater Evolution Processes Based on Hydrochemistry and Environmental Isotopes: A Case Study of the Dongyuan Drinking Water Source Area in Ji'nan City].

The Dongyuan groundwater source area, which is the main drinking water source of Ji'nan City, is karst fissure water. To identify groundwater recharge sources, the influence of surface water, hydrochemical evolution, hydrochemical and isotopic components (H and O) of groundwater and surface water samples collected from the Dongyuan groundwater source were investigated.The results showed that the hydrochemical characteristics of groundwater were similar, and the main ions were Ca, HCO, and SO.

[MicroRNA-3963 Promotes Adipogenic Differentiation of Mouse-Derived Mesenchymal Stem Cells].

Objective: To investigate the effect of MicroRNA-3963(miR-3963) on the adipogenic differentiation of mouse bone-derived mesenchymal stem cells(MSC).

Methods: MSCs were isolated from C57BL/6 mice bone fragment and transfected with miR-3963 mimic, miR-3963 inhibitor and negative control. The expression of miR-3963 and transfection efficiency were detected by q-PCR.

[Effect of MicroRNA-146b-5p on Adipogenesis of Mouse Bone Essence Derived Mesenchymal Stem Cells].

Objective: To explore the effect of MicroRNA-146b-5p (miR-146b-5p) on the mouse bone essence derived MSC adipogenic differentiation.

Methods: MSC were isolated from bone essence of C57BL/6 mice. The expression level of miR-146b-5p in the process of adipogenic differentiation of MSC was detected by q-PCR; the role of miR-146b-5p mimics or inhibitors in the process of mouse bone essence derived MSC adipogenic differentiation was analyzed through oil red staining the expression of C/EBPα and PPARγ after cultured for 14 days was detected by q-PCR; the protein level of PPARγ after miR-146b-5p transfection was detected by Western blot.

[Identification of Sulfate Sources in the Groundwater System of Zaozhuang: Evidences from Isotopic and Hydrochemical Characteristics].

Karst groundwater is an important source of water supply for the industrial and agricultural proposes and drinking water in Zaozhuang City. In recent years, with the development of industrialization and increasing of domestic water consumption, the sulfate pollution of karst groundwater has become a serious problem. 36 samples of surface and different depth of groundwater were collected in southern Zaozhuang City in August, 2014.

Mortality attributable to carbapenem-resistant Pseudomonas aeruginosa bacteremia: a meta-analysis of cohort studies.

Whether carbapenem resistance is associated with mortality in patients with Pseudomonas aeruginosa bacteremia is controversial. To address this issue, we conducted a systematic review and meta-analysis based on cohort studies. We searched PubMed and Embase databases to identify articles (up to April 2015).

[Comparative study of recruitment maneuver guided by pressure-volume curve on respiratory physiology and lung morphology between acute respiratory distress syndrome of pulmonary and extrapulmonary origin in canine models].

Objective: To determine effects of recruitment maneuver (RM) guided by pressure-volume (P-V) curve on respiratory physiology and lung morphology in canine models of acute respiratory distress syndrome of pulmonary or extrapulmonary origin (ARDSp and ARDSexp).

Methods: Twenty-four healthy dogs were randomly divided into two groups with 12 dogs each: ARDSexp and ARDSp. Each dog in ARDSexp group was injected with oleic acid 0.