Effects of seawater immersion on open traumatic brain injury in rabbit model.

We emulated instances of open traumatic brain injuries (TBI) in a maritime disaster. New Zealand rabbit animal models were used to evaluate the pathophysiological changes in open TBI with and without the influence of artificial seawater. New Zealand rabbits were randomly divided into 3 groups.

Dendrimer-coupled sonophoresis-mediated transdermal drug-delivery system for diclofenac.

The purpose of the present study was to develop a novel transdermal drug-delivery system comprising a polyamidoamine dendrimer coupled with sonophoresis to enhance the permeation of diclofenac (DF) through the skin. The novel transdermal drug-delivery system was developed by using a statistical Plackett-Burman design. Hairless male Wistar rat skin was used for the DF-permeation study.

Specific suppression of beta-secretase gene expression by short interfering RNA in SK-N-SH cells.

Objective: To test the effect of short interfering RNAs (siRNAs) of beta-site APP cleaving enzyme (BACE) on inhibiting the expression of BACE in mammalian cells.

Methods: The gene of EGFP, U6 promoter and beta-secretase targeting siRNA were cloned by PCR. The PCR products were inserted into the retrovirus plasmid pLXSN.

[Specific suppression of beta-secretase gene expression by short interfering RNA in mammalian cells].

Objective: To investigate whether short interfering RNAs(siRNAs) of beta-site APP cleaving enzyme (BACE) can inhibit the expression of BACE in mammalian cells.

Methods: The gene of EGFP, U6 promoter and beta-secretase targeting siRNA were cloned by PCR, respectively. The PCR products were inserted into plasmid pLXSN.

[Tumor-suppression effect of polyactin A combined with GM-CSF, TNF-alpha and IL-4 on cord blood mononuclear cells].

Objective: To investigate the tumor-suppression effect of PA combined with GM-CSF, TNF-alpha and IL-4 on cord blood mononuclear cells (CBMC).

Methods: The mononuclear cells were isolated from human umbilical cord blood and cultured with polyacttin A (PA), GM-CSF + TNF-alpha + IL-4 (GTI), and GTI + PA (GTIP) respectively. Six days later, surface antigen expression of the cultured cells, including CD1a and CD83, which were the specialized markers of dendritic cell (DC), were analyzed by immunohistochemistry technique.

[Construction of recombinant plasmid pYTA/Abeta-HBcAg and its expression in E.coli].

Aim: To study the expression of fusion gene Abeta-HBcAg in E.coli and detect the immunogenicity of fusion protein Abeta-HBcAg.

Methods: The Abeta-HBcAg fusion gene was cut from recombinant plasmid pBV220/Abeta-HBcAg, and was inserted into the plasmid pYTA1 to construct recombinant plasmid pYTA/Abeta-HBcAg.