Publications by authors named "Wei-Hua Wen"
Article Synopsis
- Human botulism is caused by different serotypes of botulinum neurotoxin (BoNT) and this study focused on developing an antitoxin using four human monoclonal antibodies (mAbs) targeting BoNT/C, BoNT/D, and their mosaic toxins.
- The mAbs were created from human immune libraries and showed strong binding to multiple serotypes, with three undergoing evolution to enhance their affinity.
- A combination of these mAbs demonstrated effective neutralization and has potential for therapeutic and diagnostic applications, with one derivative (NTM-1634) showing safety in a Phase 1 clinical trial.
Human botulism is most commonly caused by botulinum neurotoxin (BoNT) serotypes A, B, and E. For this work, we sought to develop a human monoclonal antibody (mAb)-based antitoxin capable of binding and neutralizing multiple subtypes of BoNT/E. Libraries of yeast-displayed single chain Fv (scFv) antibodies were created from the heavy and light chain variable region genes of humans immunized with pentavalent-toxoid- and BoNT/E-binding scFv isolated by Fluorescence-Activated Cell Sorting (FACS).
View Article and Find Full Text PDF[Genetic epidemiologic study on nasal polyps].
Zhonghua Er Bi Yan Hou Tou Jing Wai Ke Za Zhi
August 2007
Objective: To investigate the genetic mode of nasal polyp and the effect of genetic factor on occurrence of the disease.
Methods: A genetic epidemiological case-control study including 280 pedigrees (120 nasal polyp cases and 160 controls) was conducted. The segregation ratio and the heritability of nasal polyp were respectively estimated by the Li-Mantel-Gart method and the Falconer method.
[Real-time PCR used to detect p53 gene damage in workers exposed to arsenic].
Sichuan Da Xue Xue Bao Yi Xue Ban
July 2007
Objective: To evaluate the relationship between the metabolism of arsenic and the damage of exon 5 and 8 of p53 gene from workers in a arsenic mill, and with real-time PCR technique, to establish the method probing the gene-specific DNA damage in people.
Methods: By real-time PCR, the damages of exon 5 and 8 of p53 gene were probed in 37 workers exposed highly to, 16 manager and logistic employees exposed less to an arsenic mill in Yunnan province, and also 25 local people who did not contact with any white arsenic in near past time. At the same time, the urinary total and organic arsenic of workers were detected.
[Study on the association between urinary organic arsenic and 8-hydroxydeoxyguanine in workers exposed to arsenic].
Zhonghua Yu Fang Yi Xue Za Zhi
May 2007
Article Synopsis
- The study aimed to investigate the link between arsenic metabolism and DNA oxidative damage among workers in an arsenic mill in Yunnan province.
- Researchers measured urinary levels of organic arsenic and DNA damage markers (8-hydroxydeoxyguanine) in 37 highly exposed workers, 16 with mild exposure, and 28 controls with no exposure.
- Results indicated higher levels of both arsenic and DNA damage in exposed workers, particularly in females, suggesting that arsenic exposure causes significant oxidative damage to DNA.
[Experiment research on the location of DNA lesion in the nucleic acid sequence].
Sichuan Da Xue Xue Bao Yi Xue Ban
March 2007
Objective: To research on establishing a method that can detect the DNA lesion at the level of nucleic acid.
Methods: The TK6 cell was cultured and treated, the genomic DNA was extracted, and the strand cleavage was induced by the endonuclease Hinf I. Then the genomic DNA was amplified by randomized terminal linker-dependent PCR (RDPCR), and hybridized by Southern hybridization with the single-stranded probes of the exon 2 of k-ras gene.
Objective: To study interaction of mixed pesticides cypermethrin and methyl parathion on reproductive hormones, thyroid hormones, and immune functions in rats.
Methods: Eighty 2-month old Wistar rats (40 male and 40 female) were divided randomly by body weight into 8 groups. The dose 1/30 LD50 were chosen for the single or combined exposure representing respective doses of 0, cypermethrin 8.
Objective: To study dose-response relationship effects of mixed cypermethrin and methyl parathion on reproductive hormones, thyroid hormones, and immune functions in rats.
Methods: Eighty 2-month old Wistar rats (40 males and 40 females) were divided randomly by bodyweight into 4 groups. Four doses (0, 1/600 LD50, 1/135 LD50 and 1/30 LD50) were chosen for the combined exposure representing respective doses of cypermethrin 0, 0.