Publications by authors named "Wei-Fei Li"
Background: Droplet digital PCR (ddPCR) is increasingly used in diagnosing clinical pathogens, but its effectiveness in cirrhosis patients with suspected ascites infection remains uncertain.
Methods: The diagnostic performance of ddPCR was assessed in 305 ascites samples, utilizing culture and clinical composite standards. The quantitative value and potential clinical impact of ddPCR were further analyzed in patients with spontaneous bacterial peritonitis.
Metagenomic next-generation sequencing (mNGS), mostly carried out in independent clinical laboratories, has been increasingly applied in clinical pathogen diagnosis. We aimed to explore the feasibility of mNGS in clinical laboratories and analyze its potential in the diagnosis of infectious ascites. Two reference panels composed of 12 strains commonly appearing in peritonitis were constructed to evaluate the performance metrics based on in-house mNGS protocols.
View Article and Find Full Text PDF[Influence of processing factors on honey-fried Codonopsis Radix].
Zhongguo Zhong Yao Za Zhi
June 2022
Article Synopsis
- The study examined how heating and adding honey affect the chemical properties and health benefits of Codonopsis Radix when prepared through frying and honey-frying processes.
- It found that while frying slightly alters the appearance and sweetness, honey significantly changes them, with the processed products showing varied levels of important components like lobetyolin, atractylenolide Ⅲ, and oligosaccharides.
- Additionally, honey-fried Codonopsis Radix displayed the highest antioxidant activity, suggesting that both heating and honey enhancement can improve its health benefits.
Background: The prevalence of hepatitis B virus (HBV) infection is high among individuals infected with human immunodeficiency virus (HIV) in China. Both HIV and HBV can be treated with tenofovir disoproxil fumarate (TDF) and lamivudine (3TC), so we evaluated the safety and efficacy of combination antiretroviral therapy (ART) that included TDF, 3TC, and efavirenz (EFV) among ART-naive individuals who were co-infected with HIV and HBV.
Methods: One hundred HIV/HBV co-infected ARV-naive individuals were started on the regimen of TDF, 3TC, and EFV, and the levels of plasma HBV DNA, HIV RNA, and biochemical evaluation related to the function of liver and kidney were analyzed.
Aim: To study the clinical outcome of antiviral therapy in hepatitis B-related decompensated cirrhotic patients.
Methods: Three hundred and twelve patients with decompensated hepatitis B cirrhosis were evaluated in a prospective cohort. With two years of follow-up, 198 patients in the group receiving antiviral therapy with nucleos(t)ide analogues and 39 patients in the control group without antiviral treatment were analysed.
[Comparison of fluorescence quantitative PCR and direct PCR sequencing in the detection of HBV polymerase RT region].
Zhonghua Gan Zang Bing Za Zhi
September 2010
Objective: To explore the feasibility of Direct PCR sequencing in clinic and the significance of Direct PCR sequencing for retrieval treatment plan.
Methods: To address this issue, a cross-sectional study on the drug resistance in the HBV polymerase RT region was performed using Fluorescence quantitative PCR and Direct PCR sequencing in 60 chronic hepatitis B patients, who responded failure to long-term LAM and/or ADV therapy.
Result: Compared with Fluorescence quantitative PCR, Direct PCR sequencing expressed a higher positive rate (43.
[Expression and purification of TAT-HBX-EGFP fusion protein and its transmembrane distribution in mouse].
Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi
August 2008
Objective: To highly express TAT-HBX-EGFP fusion protein and study its distribution in mouse liver.
Methods: TAT-HBX-EGFP recombinant vector was constructed and fusion protein was induced by IPTG and expression in BL21; fusion protein was purified by Ni-NTA argarose, then injected into the peritoneal cavity of the mice. Distribution of fusion protein was observed by immunofluorescence.
[Study on the distribution of human immunodeficiency virus-1 subtypes in different regions of China and mother-to-child transmission].
Zhonghua Liu Xing Bing Xue Za Zhi
December 2004
Article Synopsis
- * Methods: Researchers used nested-polymerase chain reaction (nPCR) to amplify specific regions of the HIV-1 virus and then sequenced the DNA to identify different subtypes.
- * Results: The study found that certain regions of China had distinct HIV-1 subtypes, with Beijing and Shanghai exhibiting multiple subtypes while Henan and Xinjiang showed only one each. Additionally, different subtypes were linked to varying rates of mother-to-child transmission, with subtype B having a higher transmission rate than subtype C.
Background: The incidence of HIV-1-related infection diseases and the mortality of AIDS have dramatically decreased since highly active antiretroviral therapy began to be used clinically in China in 1999. And we initiated a second clinical trial using a combination of Efavirenz and Indinavir to observe the effects of the immunoreaction.
Methods: Twenty patients with laboratory-confirmed chronic HIV-1 infection were recruited.
Article Synopsis
- The study aimed to analyze the prevalence and characteristics of HIV-1 and HCV infections among intravenous drug users and illegal blood donors in China.
- Out of 239 HIV-1 infected individuals, a significant 56.9% were also positive for HCV, mainly due to drug use and illegal blood donation.
- Findings revealed diverse HIV-1 subtypes and HCV genotypes linked to different transmission methods, showing that infection patterns were more influenced by behavior than by geographical location.