Publications by authors named "Wei-Chung Luo"
Cryopreservation is important in manufacturing of cell therapy products, influencing their safety and effectiveness. During freezing and thawing, intracellular events such as dehydration and ice formation can impact cell viability. In this study, the impact of controlling the ice nucleation temperature on intracellular events and viability were investigated.
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- The lyophilization process can affect the stability of nanoparticles due to higher concentrations in the freeze-concentrate, making controlled ice nucleation an important technique in the pharmaceutical industry for uniform ice formation.
- Research focused on the stability of solid lipid nanoparticles, polymeric nanoparticles, and liposomes under varying freezing conditions, finding that the residual moisture and particle size were largely unaffected by the method of ice nucleation.
- Using trehalose as a lyoprotectant improved the long-term stability of freeze-dried liposomes compared to sucrose, showing it to be a more effective option for maintaining stability at higher temperatures.
Article Synopsis
- Solid lipid nanoparticles are effective for delivering poorly water-soluble drugs and can provide sustained release or target specific sites in the body.
- The preparation involves creating a microemulsion at high temperatures, which cools down to form solid nanoparticle suspensions.
- This method of nanotemplate engineering is cost-effective, reproducible, and suitable for scaling up production.
Impact of formulation on the quality and stability of freeze-dried nanoparticles.
Eur J Pharm Biopharm
December 2021
Freeze-drying is an effective approach to improve the long-term stability of nanomedicines. Lyoprotectants are generally considered as requisite excipients to ensure that the quality of nanoparticles is maintained throughout the freeze-drying process. However, depending on the type of nanoparticles, the needs for lyoprotectants or the challenges they face during freeze-drying may be different.
View Article and Find Full Text PDFIn vitro release studies are commonly used to assess the product performance of topical dosage forms. In such studies, the mass transport of drugs through synthetic membranes into a receiving chamber filled with a release medium is measured. The release medium is also passed through filtration membranes prior to chromatographic analysis.
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