Domest Anim Endocrinol
December 2024
Gonadotropin-releasing hormone (GnRH) vaccines have been widely used to effectively inhibit gonadal development and reproductive function. To improve the immunogenicity of GnRH, we developed and evaluated the effects of GnRH6-kisspeptin-CRM197 immunization on the reproductive function in male goats. Thirty 3-month-old male goats (n = 30) were randomly assigned to control, surgical, and immunized groups.
View Article and Find Full Text PDFThe modification of tryptophan hydroxylase (TPH) for the biosynthesis of 5-hydroxytryptophan (5-HTP) has recently become a focus of research. In this study, we established a droplet-based ultrahigh-throughput microfluidic screening platform (DTSP) to improve the industrial properties of TPH, whereas a bacterial biosensor for L-tryptophan (L-Trp) detection was engineered to improve sensitivity. The promoter pJ23111 achieved a strong negative correlation between the L-Trp concentration and the fluorescence output of the biosensor.
View Article and Find Full Text PDFInt J Radiat Oncol Biol Phys
December 2024
Purpose: To establish an artificial intelligence (AI)-empowered multistep integrated (MSI) radiation therapy (RT) workflow for patients with nasopharyngeal carcinoma (NPC) and evaluate its feasibility and clinical performance.
Methods And Materials: Patients with NPC scheduled for MSI RT workflow were prospectively enrolled. This workflow integrates RT procedures from computed tomography (CT) scan to beam delivery, all performed with the patient on the treatment couch.
Background: The aim of this study is to assess the impact of optimized modified ultrafiltration (OMUF) on the physiological parameters of infants and children undergoing cardiopulmonary bypass (CPB).
Methods: In this randomized clinical trial, 30 pediatric patients were recruited and allocated into the experimental and control groups, each comprising of 15 patients. The experimental group underwent OMUF prior to the termination of CPB and extubation, while the control group received conventional modified ultrafiltration (MUF).
Premature ovarian insufficiency (POI) has recently been reported to be linked with epigenetic changes. Previous studies have focused on the regulation of individual genes associated with ovarian function through single-gene epigenetic variations; however, there is a deficiency in the comprehensive comprehension of the epigenetic profile for POI. Therefore, we conducted a multi-omics study integrating methylation, hydroxymethylation and transcriptome sequencing analyses in cumulus cells from women with POI and their matched controls.
View Article and Find Full Text PDFBackgroundAt present, there is a lack of efficient biomarkers for the diagnosis of thyroid cancer, and the influence of natural factors such as high iodine exposure on the expression of biomarkers remains unclear.MethodsSerum samples from papillary thyroid cancer (PTC) and non-cancer controls matched 1:1 in different iodine nutritional regions were analyzed metabolomically using an ultra-high performance liquid chromatography-Orbitrap Exploris mass spectrometry (UHPLC-OE-MS) platform. Then the data were randomly divided into training and test sets in a 1:1 ratio according to the different iodine nutritional regions and different PTC status.
View Article and Find Full Text PDFThe dark coloration of industrial lignin significantly limits its potential for applications in high-value products. This work reported a practical strategy for lignin color reduction through the synergistic treatment of acidic deep eutectic solvent and isopropanol (DES-IPA). The results showed that the DES-IPA treatment could effectively remove the p-coumarate units and methoxy groups in lignin.
View Article and Find Full Text PDFDepression is underpinned by a complex pathogenesis that involves the hippocampus and dorsal raphe nucleus (DRN) of the central nervous system. Although electroacupuncture (EA) is proven to be safe and effective for alleviating depression symptoms and causes minimal side effects its underlying therapeutic mechanism remains unclear. In this study, we performed targeted metabolomics to identify metabolite alterations in the hippocampus and DRN of Wistar Kyoto (WKY) rats and elucidate the role and potential mechanism of action of EA.
View Article and Find Full Text PDFThe risk of neonicotinoid insecticides to honeybees is a global issue. Cycloxaprid (CYC) is a novel neonicotinoid insecticide with outstanding activities, good safety profiles, and no cross-resistance with other neonicotinoids. Information on the environmental risks of CYC is limited, especially its effects on honeybees.
View Article and Find Full Text PDFMedicine (Baltimore)
December 2024
Rationale: Posterior urethral valve is a rare disease, prenatal diagnosis and prognosis evaluation are particularly important.
Patient Concerns: A 25-year-old pregnant woman was found enhanced parenchymal echo in both kidneys, subcapsule urinary cyst formation in both kidneys, bladder enlargement of the fetus during prenatal ultrasonography at 25 W + 4 of gestation. It was accompanied by fetal pericardial effusion and oligohydramnios.
World J Psychiatry
December 2024
Background: Eating disorders (EDs) have increasingly become a public health problem globally, especially among children and adolescents.
Aim: To estimate the burden of EDs in children and adolescents (ages 5-19 years) at the global, regional, and national levels.
Methods: Retrieved from Global Burden of Disease Study 2019 for EDs, including anorexia nervosa and bulimia nervosa, we extracted the disability-adjusted life years (DALYs) and prevalence rates with 95% uncertainty intervals between 1990-2019.
In this work, a bilayer lead-free perovskite ferroelectric structure was fabricated comprising a highly polar BiFeO (BFO) bottom layer and a less polar (KNa)NbO (KNN) top layer. The BFO sublayer, deposited via radio frequency magnetron sputtering without postgrowth annealing, not only exhibited enhanced crystallinity but also promoted superior microstructural properties in the sol-gel derived KNN overlayer, thereby ensuring excellent intrinsic electrical properties. Compared to the poorly crystallized single-layer KNN films directly synthesized on LNO-buffered (100)-Si substrate, the KNN layer in the bilayer structure demonstrated a strong (100) texture, along with a dense, homogeneous, fine-grained morphology.
View Article and Find Full Text PDFBats are presumed reservoirs of diverse coronaviruses (CoVs) including progenitors of Severe Acute Respiratory Syndrome (SARS)-CoV and SARS-CoV-2, the causative agent of COVID-19. However, the evolution and diversification of these coronaviruses remains poorly understood. Here we use a Bayesian statistical framework and a large sequence data set from bat-CoVs (including 589 novel CoV sequences) in China to study their macroevolution, cross-species transmission and dispersal.
View Article and Find Full Text PDFExosomes have emerged as significant biomarkers for multiple diseases, including cancers. Circular RNAs (circRNAs), abundant in exosomes, are involved in regulating cancer development. However, the regulatory function and the underlying molecular mechanism of hsa_circ_0006906 (circSCP2) in colorectal cancer (CRC) metastasis remain unclear.
View Article and Find Full Text PDFBackground: A substantial number of hepatitis C virus (HCV) infections have been diagnosed and reported, yet not all reported patients have received treatment, leading to uncertainties in the progression of the virus within these cases. This study aimed to assess the rate of HCV RNA positivity in the reported cases in Chongqing, China and identify key groups.
Methods: An investigation was conducted on 6,333 hepatitis C cases who were reported in the China Information System for Disease Control and Prevention in 17 districts of Chongqing, China from 2004 to 2021.
BMC Musculoskelet Disord
December 2024
Introduction: Using the data taken from the National Health and Nutrition Examination Survey (NHANES) database, we explored the relationship between the dietary inflammatory index (DII) and low muscle mass (LMM) in adults.
Methods: We examined 20,424 adults ≥ 20 years old from the 1999-2006 and 2011-2018 NHANES. We hypothesized that the DII is positively associated with the risk of LMM in adults.
Background: Low back pain is a common complication in patients with old thoracolumbar fractures with kyphosis (OTLFK), and intervertebral disc degeneration (IDD) is a major contributor. Mechanical abnormalities are believed to play a key role in the development to IDD. This study aimed to investigate the characteristics of lumbar disc degeneration and underlying mechanical mechanisms in patients with OTLFK.
View Article and Find Full Text PDFBackground: The role of dietary and nutritional factors in inflammatory bowel disease (IBD) remains poorly understood, necessitating further investigation. This study aims to explore the association between nutrient intake and the risk of IBD.
Methods: This cross-sectional study utilized data from the 2009-2010 NHANES cycle, focusing on individuals with complete 24-hour dietary intake records and clinically assessed IBD information.
Background: Characterized by its high mortality and easy recurrence, hepatocellular carcinoma (HCC) poses significant clinical challenges. The association between copper metabolism and development of cancer has been identified. However, the underlying mechanisms of copper metabolism-related long non-coding RNAs (CMRLs) in HCC remain elusive.
View Article and Find Full Text PDFBackground: Ischemic stroke (IS) is a significant global health issue, causing high rates of morbidity, mortality, and disability. Since conventional Diagnosis methods for IS have several shortcomings. It is critical to create new Diagnosis models in order to enhance existing Diagnosis approaches.
View Article and Find Full Text PDFHepatocellular carcinoma (HCC) is recognized as a highly malignant tumor. Targeted combination immunotherapy, the initially approved regimen, is compromised by adverse side effects and low response rates during clinical treatment. Traditional Chinese medicine and its derived natural compounds, known for their anticancer effects, offer advantages of low toxicity and cost.
View Article and Find Full Text PDFBackground: Empathy is essential in medical practice, particularly in obstetrics and gynecology. Narrative Medicine, which involves storytelling and reflective writing, has been shown to enhance empathy among healthcare providers. Despite its benefits, there is limited research on the impact of narrative medicine on empathy among obstetrics and gynecology residents.
View Article and Find Full Text PDFRecent developments in spatially resolved transcriptomics (SRT) enable the characterization of spatial structures for different tissues. Many decomposition methods have been proposed to depict the cellular distribution within tissues. However, existing computational methods struggle to balance spatial continuity in cell distribution with the preservation of cell-specific characteristics.
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