Loss of LRRK2 activity induces cytoskeleton defects and oxidative stress during porcine oocyte maturation.

Leucine-rich repeat kinase 2 (LRRK2) is a ROCO family member which its mutation is closely related with Parkinson's disease, and LRRK2 is widely involved into the regulation of autophagy, vesicle transport and neuronal proliferation. However, the roles of LRRK2 during mammalian oocyte maturation are still largely unclear. In present study, we disturbed the activity of LRRK2 and showed its essential roles in porcine oocytes.

Splenic subcapsular hematoma following endoscopic retrograde cholangiopancreatography: A case report and review of literature.

Background: Splenic injury following endoscopic retrograde cholangiopancreatography (ERCP) is a rare complication. The literature contains around 30 articles reporting various degrees of splenic injuries resulting from ERCP since the first report of splenic rupture after ERCP in 1989.

Case Summary: This report describes a case of splenic hematoma and stent displacement in a 69-year-old male patient who developed these conditions 7 days after undergoing ERCP and stenting.

Uncovering the ceRNA network and DNA methylation associated with gene expression in nasopharyngeal carcinoma.

Objective: This study aimed to uncover abnormally expressed genes regulated by competitive endogenous RNA (ceRNA) and DNA methylation nasopharyngeal carcinoma and to validate the role of lncRNAs in the ceRNA network on nasopharyngeal carcinoma progression.

Methods: Based on the GSE64634 (mRNA), GSE32960 (miRNA), GSE95166 (lncRNA), and GSE126683 (lncRNA) datasets, we screened differentially expressed mRNAs, miRNAs and lncRNAs in nasopharyngeal carcinoma. A ceRNA network was subsequently constructed.

Acrylamide Exposure Destroys the Distribution and Functions of Organelles in Mouse Oocytes.

Acrylamide (ACR) is a common industrial ingredient which is also found in foods that are cooked at high temperatures. ACR has been shown to have multiple toxicities including reproductive toxicity. Previous studies reported that ACR caused oocyte maturation defects through the induction of apoptosis and oxidative stress.

[Correlation of single nucleotide polymorphisms of the osteopontin gene with astheozoospermia].

Objective: To investigate the correlation of the single nucleotide polymorphisms (SNP) rs1126772, rs117291487, rs11730582, rs142608941 and rs6813526 of the osteopontin (OPN) gene with the risk of asthenozoospermia (AZS).

Methods: We included 135 AZS patients in the AZS group and another 239 fertile men as normal controls. Using the SNaPshot technique, we genotyped the rs1126772, rs117291487, rs11730582, rs142608941 and rs6813526 polymorphisms of the OPN gene in all the subjects and analyzed the correlation of the five SNPs with AZS.

LncRNA HOTAIR regulates the expression of E-cadherin to affect nasopharyngeal carcinoma progression by recruiting histone methylase EZH2 to mediate H3K27 trimethylation.

Background/aim: There has been increasing evidence for the function of long non-coding RNA (lncRNA) in nasopharyngeal carcinoma (NPC). We aim to delve into the position of lncRNA HOX antisense intergenic RNA (HOTAIR), together with enhancer of zeste homolog 2 (EZH2), E-cadherin and trimethylation of lysine 27 on histone H3 (H3K27me3) in NPC.

Methods: HOTAIR, EZH2, and E-cadherin expression in NPC tissues and cells were tested.

Podophyllotoxin Exposure Causes Spindle Defects and DNA Damage-Induced Apoptosis in Mouse Fertilized Oocytes and Early Embryos.

Podophyllotoxin (PPT) is a kind of lignans extracted from the roots and stems of the genus from the tiller family, and it has been widely used in the treatment of condyloma acuminatum, multiple superficial epithelioma in the clinics. However, PPT has been reported to be toxic and can cause liver defects and other organ poisoning. In addition, emerging evidences also indicate that PPT has reproductive toxicity and causes female reproduction disorders.

Effects of Polysaccharide on Endoplasmic Reticulum Stress and Oxidative Stress in Obese Mice.

Background: The incidence of obesity-associated decline in male fertility has increased over the years. polysaccharide (LBP), a natural plant polysaccharide extracted from the Chinese herb has shown promising therapeutic effects in overcoming the same.

Aim: This study aimed to investigate the protective effect of LBP on the testes of obese mice.

Exosomal lncRNA HNF1A-AS1 affects cisplatin resistance in cervical cancer cells through regulating microRNA-34b/TUFT1 axis.

Background: There is growing evidence of the role of long non-coding RNAs (lncRNAs) in cervical cancer (CC). The objective was to discuss whether exosomal lncRNA HNF1A-AS1 impacted drug resistance in CC via binding to microRNA-34b (miR-34b) and regulating TUFT1 expression.

Methods: The expression of HNF1A-AS1 in normal cervical epithelial cells, cisplatin (DDP)-sensitive cell line (HeLa/S) and DDP-resistant cell line (HeLa/DDP) cells were detected.

Association of interleukin-27 gene polymorphisms with susceptibility to HIV infection and disease progression.

Interleukin-27 (IL-27) gene polymorphisms are linked to infectious disease susceptibility and IL-27 plasma level is associated with HIV infection. Therefore, we aimed to investigate the association between IL-27 polymorphisms and susceptibility to HIV infection and disease progression. A total of 300 patients with HIV infection (48 long-term nonprogressors and 252 typical progressors) and 300 healthy controls were genotyped for three IL-27 polymorphisms, rs17855750, rs181206, rs40837 which were performed by using multiple single nucleotide primer extension technique.

Genetic association of promoter in GRP78 gene with nasopharyngeal carcinoma in a Chinese population.

Background: Emerging evidences were accumulated to support the view that GRP78 might be associated with multiple types of cancer. Given these, the aim of this study is to investigate the relationship between single nucleotide polymorphisms (SNPs) of GRP78 gene promoter and nasopharyngeal carcinoma (NPC).

Methods: Three SNPs (rs3216733, rs17840761 and rs17840762) in GRR78 promoter were estimated in 422 NPC patients and 452 controls.

[Asthenozoospermia is not correlated with 3' UTR polymorphisms of the GRP78 gene].

Objective: To investigate the correlation of the single nucleotide polymorphisms (SNPs) rs12009, rs1140763 and rs16927997 in the 3'-untranslated region (3'UTR) of the glucose-regulated protein 78 (GRP78) gene with the risk of male asthenozoospermia (AZS).

Methods: We included 400 AZS patients in the AZS group and another 400 fertile men as normal controls. Using the SNaPshot technique, we genotyped the rs12009, rs1140763 and rs16927997 polymorphisms in the 3'UTR of the GRP78 gene in all the male subjects and analyzed the association of the three SNPs with AZS.

[Revision process and thinking of emission standard of air pollutants for cement industry].

The new National Emission Standard of Air Pollutants for Cement Industry (GB 4915-2013) was released recently, which is the third revision since the first release in 1985. This paper reviewed the revision process for the emission standard of air pollutants in cement industry, analyzed the impact of environmental protection situation and management policies changes on the content and form of the standard. The standard formulating principles and several key issues together constitute the base of emission standard, which are not only important to complete the theories and methods of emission standard development, but also important to improve the environmental management and pollution control level.

[A comparative study on domestic and foreign emission standards of air pollutants for cement industry].

The new National Emission Standard of Air Pollutants for Cement Industry (GB 4915-2013) becomes effective on Mar. 1st, 2014. It will play an important role in pollution prevention, total emission reduction, structure adjustment, and layout optimization for cement industry.

[Study on feasible emission control level of air pollutions for cement industry ].

The revised National Emission Standard of Air Pollutions for Cement Industry has been issued, which will be effective for the new enterprises and the existing enterprises on Mar. 1st, 2014 and July 1st, 2015, respectively. In the process of revision, the key technical issues on determination of standard limits was how to determine the feasible emission control level of air pollutions.

[Establishment and improvement of emission control standard system of volatile organic compounds in industry].

Volatile organic compounds (VOCs) has become one of the priority control pollutants, due to the regional compound pollution problem represented by atmospheric haze. Through the analysis of the present situation for current national and local emission standards of VOCs, the pollution characteristics and the emission inventory of VOCs, a basic standard system of VOCs has been proposed and improved.

[Study on key technical problems in the development of volatile organic pollutants emission standards].

This paper analyzes four key technical problems in the development of volatile organic pollutants emission standards, which are the selection of pollution factors, determination of control indicators, determination of emission limits and the technical management measures. Pollution factors should be taken into account, according to toxicity and photochemical reactivity. Both organized and unorganized control indicators should be considered to determine control indicators.

[Study on emission standard system of air pollutants].

Scientific and reasonable emission standard system of air pollutants helps to systematically control air pollution, enhance the protection of the atmospheric environment effect and improve the overall atmospheric environment quality. Based on the study of development, situation and characteristics of national air pollutants emission standard system, the deficiencies of system were pointed out, which were not supportive, harmonious and perfect, and the improvement measures of emission standard system were suggested.

Molecular cloning, characteristics and low temperature response of raffinose synthase gene in Cucumis sativus L.

Raffinose synthase (RS, EC2.4.1.

[Emission control way of volatile organic compounds in industry].

Due to the volatile nature, the way of controlling way of VOCs was different from other atmospheric pollutants. By analyzing the emission characteristics of VOCs, four kinds of control way were proposed, which were the source control, organized emission control, fugitive emission control and the total amount control, and the control modes of each control way were also analyzed and compared.

[Study on the fluorescence characteristics of the interaction between DNA and two halogenated fluoresceins].

The fluorescence characteristics of the interaction between DNA and two halogen fluoresceins, tetrabromofluorescein (TBF) and tetrachlorotetrabromofluorescein (TTF), were studied. The result showed that for TBF, lamda ex/ lamda em were 518 nm/540 nm, and for TTF, they were 540 nm/560 nm. The intensity of fluorescence of TBF and TTF changed in the presence of DNA.

[Study on the interaction of tetrabromofluorescein and DNA].

The interaction of tetrabramofluorescein (TBF) and DNA was studied by the fluorescence and SS-RTP. The effect of the ion strength and ethanol on the fluorescence spectra of TBF in the presence of DNA and absence of DNA was investigated. In addition, the effect of pH on the SS-RTP spectra of TBF was also examined.