A rhizobacterium-secreted protein induces lateral root development through the IAA34-PUCHI pathway.

Lateral roots (LRs) can continuously forage water and nutrients from soil. In Arabidopsis thaliana, LR development depends on a canonical auxin signaling pathway involving the core transcription factors INDOLE-3-ACETIC ACIDs (IAAs) and AUXIN RESPONSE FACTORs (ARFs). In this study, we identified a protein, bacillolysin, secreted by the beneficial rhizobacterium Bacillus velezensis SQR9, that is able to stimulate LR formation of Arabidopsis.

Harnessing machine learning and AI-driven analytics to identify novel drug targets and predict chemotherapy efficacy in NSCLC.

Introduction: Non-small cell lung cancer (NSCLC) constitutes the majority of lung cancer cases and exhibits marked heterogeneity in both clinical presentation and molecular profiles, leading to variable responses to chemotherapy. Emerging evidence suggests that mitochondria-derived RNAs (mtRNAs) may serve as novel biomarkers, although their role in predicting chemotherapy outcomes remains to be fully explored.

Methods: In this study, peripheral blood mononuclear cells were obtained from NSCLC patients for analysis of the mtRNA ratio (mt_tRNA-Tyr-GTA_5_end to mt_tRNA-Phe-GAA), while thoracic CT images were processed to derive an AI-driven BiomedGPT variable.

Alterations of diffusion kurtosis measures in gait-related white matter in the "ON-OFF state" of Parkinson's disease.

Background: Gait impairment is closely related to quality of life in patients with Parkinson's disease (PD). This study aimed to explore alterations in brain microstructure in PD patients and healthy controls (HCs) and to identify the correlation of gait impairment in the ON and OFF states of patients with PD, respectively.

Methods: We enrolled 24 PD patients and 29 healthy controls (HCs) from the Movement Disorders Program at Beijing Friendship Hospital Capital Medical University between 2019 and 2020.

SIRT1 Alleviates Mitochondrial Fission and Necroptosis in Cerebral Ischemia/Reperfusion Injury via SIRT1-RIP1 Signaling Pathway.

Programmed cell death, including necroptosis, plays a critical role in the pathogenesis of cerebral ischemia/reperfusion injury (CIRI). Silent information regulator 1 (SIRT1) has been identified as a potential therapeutic target for CIRI, yet its precise role in regulating necroptosis remains controversial. Furthermore, the potential interaction between SIRT1 and receptor-interacting protein kinase 1 (RIP1) in this context is not fully understood.

Engineered platelets-based nano-aircraft system for precise tumor chemo-immunotherapy with graded drug delivery and self-recognized tumor targeting.

Metastasis and heterogeneity pose major challenges in cancer treatment. Although chemoimmunotherapy shows promising efficacy, its therapeutic impact is limited by off-target effects and differences in the delivery sites of chemotherapeutic drugs and immunosuppressants. In this study, an engineered platelets (Pts)-based nano-aircraft, Pts@DOX/HANGs@Gal, was constructed with an internally loaded chemotherapeutic drug, doxorubicin, and externally grafted reduction-responsive hyaluronidase-cross-linked nanospheroids loaded with the immunosuppressant galunisertib for precise tumor chemo-immunotherapy.

Identification of benzo(a)pyrene-related toxicological targets and their role in chronic obstructive pulmonary disease pathogenesis: a comprehensive bioinformatics and machine learning approach.

Background: Chronic obstructive pulmonary disease (COPD) pathogenesis is influenced by environmental factors, including Benzo(a)pyrene (BaP) exposure. This study aims to identify BaP-related toxicological targets and elucidate their roles in COPD development.

Methods: A comprehensive bioinformatics approach was employed, including the retrieval of BaP-related targets from the Comparative Toxicogenomics Database (CTD) and Super-PRED database, identification of differentially expressed genes (DEGs) from the GSE76925 dataset, and protein-protein interaction (PPI) network analysis.

Mapping the knowledge of omics in myocardial infarction: A scientometric analysis in R Studio, VOSviewer, Citespace, and SciMAT.

Many researchers nowadays choose multi-omics techniques for myocardial infarction studies. However, there's yet to be a review article integrating myocardial infarction multi-omics. Hence, this study adopts the popular bibliometrics.

α-asarone activates mitophagy to relieve diabetic encephalopathy via inhibiting apoptosis and oxidative stress.

Diabetic encephalopathy (DE) is a common complication of diabetes that may result in cognitive impairment. Currently, there is limited effective therapy for DE. Herein, we explored the beneficial effect of α-Asarone on DE and its potential mechanisms.

Regulation of Hippo-YAP1/TAZ pathway in metabolic dysfunction-associated steatotic liver disease.

Metabolic dysfunction-associated steatotic liver disease (MASLD) has become the most prevalent chronic liver disease worldwide, but effective treatments are still lacking. Metabolic disorders such as iron overload, glycolysis, insulin resistance, lipid dysregulation, and glutaminolysis are found to induce liver senescence and ferroptosis, which are hot topics in the research of MASLD. Recent studies have shown that Hippo-YAP1/TAZ pathway is involved in the regulations of metabolism disorders, senescence, ferroptosis, inflammation, and fibrosis in MASLD, but their complex connections and contrast roles are also reported.

Multifunctional injectable hydrogel with self-supplied HS release and bacterial inhibition for the wound healing with enhanced macrophages polarization via interfering with PI3K/Akt pathway.

Hydrogen sulfide (HS) gas therapy is beneficial for accelerating wound healing and alleviating the inflammatory process, but is seriously hindered by insufficient delivery and unsustainable release in vivo. This study presents a multifunctional injectable hydrogel, OC@ε-PL-SATO, composed of oxidized hyaluronic acid and N-acetylcysteine (NAC) as an initiator, carboxymethyl chitosan and S-aroylthiooxime modified ε-Poly-(l-lysine) (ε-PL-SATO). ε-PL-SATO is a NAC-responsive HS donor.

Using resting-state functional magnetic resonance imaging and contrastive learning to explore changes in the Parkinson's disease brain network and correlations with gait impairment.

Background: There are currently no deep learning models applying resting-state functional magnetic resonance imaging (rs-fMRI) data to distinguish patients with Parkinson's disease (PD) and healthy controls (HCs). Moreover, no study has correlated objective gait parameters with brain network alterations in patients with PD. We propose BrainNetCNN + CL, applying a convolutional neural network (CNN) and joint contrastive learning (CL) method to brain network analysis to classify patients with PD and HCs, and compare their performance with classical classification methods.

Prediction and influence factors analysis of IP backbone network traffic based on Prophet model and variance reduction.

Accurate and efficient traffic prediction directly determines the construction scale and investment budget of communication networks, which is crucial for network planning. Despite the rise of popular machine learning models, traditional statistical models maintain significant advantages in interpretability, controllability and simplicity, retaining an essential role in contemporary communication network traffic prediction. This paper analyzes and predicts the inter-provincial egress traffic of 31 provinces in a large-scale operational IP backbone network using traditional regression analysis, the time series Prophet model, and a novel combination of these two prediction models.

White Matter Fiber Bundle Alterations Correlate with Gait and Cognitive Impairments in Parkinson's Disease based on HARDI Data.

Background: The neuroanatomical basis of white matter fiber tracts in gait impairments in individuals suffering from Parkinson's Disease (PD) is unclear.

Methods: Twenty-four individuals living with PD and 29 Healthy Controls (HCs) were included. For each participant, two-shell High Angular Resolution Diffusion Imaging (HARDI) and high-resolution 3D structural images were acquired using the 3T MRI.

A CNN-based self-supervised learning framework for small-sample near-infrared spectroscopy classification.

Near-infrared (NIR) spectroscopy, with its advantages of non-destructive analysis, simple operation, and fast detection speed, has been widely applied in various fields. However, the effectiveness of current spectral analysis techniques still relies on complex preprocessing and feature selection of spectral data. While data-driven deep learning can automatically extract features from raw spectral data, it typically requires large amounts of labeled data for training, limiting its application in spectral analysis.

Sinomonas gamaensis NEAU-HV1 remodels the IAA14-ARF7/19 interaction to promote plant growth.

Sinomonas species typically reside in soils or the rhizosphere and can promote plant growth. Sinomonas enrichment in rhizospheric soils is positively correlated with increases in plant biomass. However, the growth promotion mechanisms regulated by Sinomonas remain unclear.

Electrospray whey protein-polysaccharide microcapsules co-encapsulating Lactiplantibacillus plantarum and EGCG: Enhanced probiotic viability and antioxidant activity.

Probiotics are often subjected to adverse factors during processing, storage and digestion. To enhance the viability and function of probiotics, whey protein concentrate (WPC)-based microcapsules were systematically fabricated to co-encapsulate probiotic Lactiplantibacillus plantarum KLDS 1.0328 (LP KLDS 1.

Wearable Sensor Based on Covalent Organic Framework Humidity Films for Long-Term Monitoring of Tomato Physiology Under Abiotic Stress.

Global agricultural productivity is affected by plant stresses every year; as a consequence, monitoring and preventing plant stresses is a significant measure to protect the agro-ecological environment. Similar to the adoption of wearable devices to appraise human physiological information and disease diagnosis, however, in situ nondestructive monitoring of complex and weak physiological information in plants is an enormous challenge for the development of wearable sensors. Herein, to accurately analyze the changes of tomato internal information under multiple abiotic stresses in real-time, we introduce the covalent organic framework (COF) film synthesized by self-assembly layer by layer through the oil/water interface as a sensitive material to develop a multifilm-integrated wearable sensor capable of monitoring leaf surface humidity and leaf temperature.

[Establishment of BCL-2 Inhibitors-Resistant B-cell Acute Lymphoblastic Leukemia Cell Lines and Study on Their Resistance Mechanisms].

Objective: RS4;11 cell line was used to establish BCL-2 inhibitor-resistant cell lines of B-cell acute lymphoblastic leukemia (B-ALL) and explore the possible mechanisms of drug resistance.

Methods: RS4;11 cell line was continuously induced and cultured by low and ascending concentrations of BCL-2 inhibitors navitoclax and venetoclax to construct navitoclax-resistant cell line RS4;11/Nav and venetoclax-resistant cell line RS4;11/Ven. The cell viability was detected by MTT assay, and the cell apoptosis was detected by flow cytometry.

[Comparative study of total hip arthroplasty with and without femoral osteotomy in Crowe Ⅳ developmental dysplasia of the hip].

Objective: To compare the clinical effects of total hip arthroplasty(THA) with and without femoral osteotomy in Crowe Ⅳ developmental hip dislocation(DDH).

Methods: The data on 46 patients who underwent THA for unilateral Crowe Ⅳ DDH between 2012 and 2017 were analyzed retrospectively. They were divided into two groups according to the different surgical methods.

A review of botany, ethnomedicine, phytochemistry, pharmacology and toxicology of Sarcandra species.

Background: Sarcandra is one of the five genera of Chloranthaceae, which has a long history of medicinal use and high medicinal value, with excellent therapeutic effects on liver cancer, pneumonia, colitis, bone fractures, and dysentery. Among its species, Sarcandra glabra (Thunb.) Nakai has been extensively utilized in diverse compound formulations, toothpaste, tea, daily commodities, as well as health supplements.

A nitrogen-responsive cytokinin oxidase/dehydrogenase regulates root response to high ammonium in rice.

Plant root system is significantly influenced by high soil levels of ammonium nitrogen, leading to reduced root elongation and enhanced lateral root branching. In Arabidopsis, these processes have been reported to be mediated by phytohormones and their downstream signaling pathways, while the controlling mechanisms remain elusive in crops. Through a transcriptome analysis of roots subjected to high/low ammonium treatments, we identified a cytokinin oxidase/dehydrogenase encoding gene, CKX3, whose expression is induced by high ammonium.