Three new metabolites from the endophyte T2-10.

One new cyclopeptide, cyclo-(-Trp--Phe--Phe) (), one new 2-pyridone derivative, fusarone A (), and one new natural indole derivative, ethyl 3-indoleacetate (), along with six known compounds were isolated from the endophytic fungus T2-10. The planar structures of three new compounds were identified by spectral methods including 1D and 2D NMR techniques, and the absolute configuration of compound was elucidated by Marfey-MS method. In addition, all compounds were evaluated for their cytotoxic and antibacterial activities .

Two new chromone derivatives from the rhizosphere soil fungus .

Two new chromone derivatives ( and ), and two known compounds ( and ) were isolated from the rhizosphere soil fungus . Their planar structures and absolute configurations were determined by extensive spectroscopic analysis and electronic circular dichroism (ECD) calculations. Additionally, all the isolated compounds were evaluated for their antibacterial activity against , , and , but no obvious activity was observed at a concentration of 128 μg/mL.

Pseudocercones A-C, three new polyketide derivatives from the endophytic fungus sp. TSS-1.

Chemical investigation of an EtOAc extract of the endophytic fungus sp. TSS-1 led to the isolation of three new polyketide derivatives, including one benzophenon derivative (), two spirocyclic polyketides ( and ), along with four known compounds (, , and ). Their structures and the absolute configurations were characterized by means of NMR, HRESIMS, C NMR and theoretical electronic circular dichroism calculations.

β-catenin correlates with the progression of colon cancers and berberine inhibits the proliferation of colon cancer cells by regulating the β-catenin signaling pathway.

Introduction: Berberine was one of the active components in Chinese herb and exerted tumor suppressive role in cancer progression, but the exact antitumor mechanism is still not clearly clarified. In the present study, bioinformatics analysis was performed on COAD patients from TCGA, HPA database, UALCAN and GEPIA 2 platform. We also explored the role of berberine on progression of human colon cancers in vitro and in vivo and clarified weather the antitumor effects of berberine was mediated by Wnt/beta-catenin pathway.

[Effect of serum starvation and culture to confluence on cell cycle synchronization and mineralization of human dental pulp cells].

Purpose: To compare the effect of serum starvation and culture to confluence on cell cycle synchronization and mineralization of human dental pulp cells (hDPCs).

Methods: HDPCs were cultured to 80% and 100% confluence respectively, and then cultured for 24, 48 and 72 hours by culture medium containing 0.5% fetal bovine serum(FBS).

[Mechanisms and applications of transcutaneous electrical nerve stimulation in analgesia].

Transcutaneous electrical nerve stimulation (TENS), as a non-pharmacological and non-invasive analgesic therapy with low-cost, has been widely used to relieve pain in various clinical applications, by delivering current pulses to the skin area to activate the peripheral nerve fibers. Nevertheless, analgesia induced by TENS varied in the clinical practice, which could be caused by the fact that TENS with different stimulus parameters has different biological mechanisms in relieving pain. Therefore, to advance our understanding of TENS in various basic and clinical studies, we discussed (1) neurophysiological and biochemical mechanisms of TENS-induced analgesia; (2) relevant factors that may influence analgesic effects of TENS from the perspectives of stimulus parameters, including stimulated position, pulse parameters (current intensity, frequency, and pulse width), stimulus duration and used times in each day; and (3) applications of TENS in relieving clinical pain, including post-operative pain, chronic low back pain and labor pain.

Room-temperature soft mode and ferroelectric like polarization in SrTiO ultrathin films: Infrared and ab initio study.

Due to the remarkable possibilities of epitaxially growing strontium titanate (SrTiO or STO) on silicon, this oxide is widely used as a buffer layer for integrating other perovskite oxides which allows for the development of various functional electronic devices on silicon. Moreover, STO is known to be an incipient ferroelectric in bulk but may become ferroelectric when in the form of strained ultrathin films. Given the importance of the potential applications for electronics if this property is demonstrated, we performed a spectroscopic study of STO on Si(001) templates coupling experimental and ab initio investigations.

[The Research Advances of the Pathomechanism of Phantom Limb Pain (PLP)].

Phantom limb pain (PLP) is a hallucination that the patient feels the existence off the limb after its loss and experiences somewhat pain of the missing limb. Such a pain normally appears in the distal end of the missing limb. Currently, the pathomechanism of PLP is still unclear, and the clinical research of PLP mainly relies on the subjective report of the patients and the psychophysical measurements.

Effects of deferoxamine on the repair ability of dental pulp cells in vitro.

Introduction: In previous studies, we found that hypoxia promoted the mineralization of dental pulp cells (DPCs). However, the clinical application of hypoxia as a therapy is questionable or unfeasible. Deferoxamine (DFO), a medication for iron overload, has also been shown to induce hypoxia.

Isolation and identification of CXCR4-positive cells from human dental pulp cells.

Introduction: In previous studies, we found expression of stromal cell-derived factor-1α (SDF-1α)/CXC chemokine receptor 4 (CXCR4) in human dental pulp and the SDF-1α-CXCR4 axis might play a role in the recruitment of CXCR4-positive dental pulp cells (CXCR4(+) DPCs) toward the damaged sites. However, the specific function of CXCR4(+) DPCs in the injured dental pulp was still unknown. The purpose of this study was to isolate CXCR4(+) DPCs from dental pulp cells in vitro to pave the way for further study of their characteristics.

Proliferation and multilineage potential of CXCR4-positive human dental pulp cells in vitro.

Introduction: Although the results of our previous studies showed that the stromal cell-derived factor (SDF)-1α-CXC chemokine receptor 4 (CXCR4) axis may play a role in the recruitment of CXCR4-positive dental pulp cells (CXCR4(+) DPCs) toward the damaged sites, the specific function of CXCR4(+) DPCs in the injured dental pulp was still unknown. The purpose of this study was to verify whether CXCR4(+) DPCs possessed stem cells properties so that we can understand their role in area of injury.

Methods: CXCR4(+) DPCs were isolated from normal DPCs with magnetic-activated cell sorting.

Gastrostomy tube placement by radiological versus endoscopic methods in an acute care setting: a retrospective review of frequency, indications, complications and outcomes.

Objectives: To describe the current practice of placing gastrostomy tubes (endoscopic and radiological), patient characteristics, indications for enteral support, complications and outcomes over a 13-month period, and explore factors that influenced complications and outcomes. Second, to provide Canadian data regarding feeding tube placement because no current literature reflecting these practices for Canadian hospitals is available.

Methods: Retrospective chart reviews were conducted.