Publications by authors named "Wei Rong Zhai"
The aim of the present study was to explore the relationship between methylation status of the p16(INK4A) promoter and some HBV-related factors, and the role of these factors in p16(INK4A) hypermethylation and hepatocellular carcinoma (HCC) progression. Twenty-three cases of surgically resected HBV-associated HCC and 25 fine-needle aspiration biopsy cases of chronic hepatitis B (CHB) were studied. The methylation status of the p16(INK4A) promoter was determined by methylation-specific polymerase chain reaction (PCR).
View Article and Find Full Text PDFChanges of integrin expression in rat hepatocarcinogenesis induced by 3'-Me-DAB.
World J Gastroenterol
April 2000
Article Synopsis
- * Researchers used a method called immunohistochemistry to observe specific integrins (alpha(1), alpha(2), alpha(3), and alpha(5)) and epidermal keratin in liver tissues under different conditions, from normal to cancerous.
- * Results showed that while integrin expression decreases in cancerous tissues, integrins alpha(1) and alpha(5) are more prevalent in metastatic (spread) tumors, suggesting these integrins might be significant for liver cancer
AIM:To find out the relationship between the gene transcription of different types of procollagen and the deposition of the relevant collagens in the liver tissue and to confirm the types of collagen producing cells in liver fibrogenesis.METHODS:Dynamic changes of the expression of alpha1(I), alpha1(III) and alpha1(IV) procollagen mRNA and relevant collagens and the distribution of collagen producing cells during liver fibrogenesis of rat induced by CCl(4) (20 weeks) were investigated with Northern blot analysis, in situ hybridization and immunohistochemical techniques.RESULTS: The increased expression of alpha 1(III) procollagen mRNA by Northern blot analysis was the most predominant one among the three mRNAs during fibrogenesis.
View Article and Find Full Text PDFStudy of heteroserum-induced rat liver fibrosis model and its mechanism.
World J Gastroenterol
June 1998
Article Synopsis
- The study aimed to explore how liver fibrosis develops in rats after injecting porcine serum and to understand the underlying mechanisms involved in this process.
- Researchers established a rat model of liver fibrosis and observed morphological changes, while quantitatively measuring the infiltration of immune cells and detecting specific proteins using immunofluorescence.
- Results showed successful fibrosis induction by the 8th week, highlighting the role of hepatic stellate cells and primary mesenchyma cells, with indications that the early immune response and a late phase allergic reaction contribute to the pathogenesis of the condition.
Replicative efficiency and pathogenicity of hepatitis B virus e-minus precore variant.
J Gastroenterol Hepatol
November 1998
To study the replicative efficiency and pathogenicity of hepatitis B virus precore variant (A1896), anti-hepatitis B virus e antigen (HBe) titre was studied in naturally occurring wild-type virus infection, A1896 variant infection and dual infection. Higher titre of anti-HBe was found in patients with no virus replication and in patients coinfected with the wild-type virus and A1896 variant, which suggest that anti-HBe may either act as an inhibitor of virus replication or as selective pressure for the A1896 variant. Three site-directed mutants were constructed in the duck hepatitis B virus (DHBV) precore region.
View Article and Find Full Text PDF