Proteomic characterization of esophageal squamous cell carcinoma response to immunotherapy reveals potential therapeutic strategy and predictive biomarkers.

Immunotherapy is the first-line therapy for esophageal squamous cell carcinoma (ESCC), yet many patients do not respond due to drug resistance and the lack of reliable predictive markers. We collected 73 ESCC patients (including discovery cohort and validation cohort) without immune thrombocytopenia and undergoing anti-PD1 immunotherapy. Proteomic and phosphoproteomic analysis of 73 ESCC treatment-naive samples by mass spectrometry-based label-free quantification were applied to explore the potential resistant and sensitive mechanisms, and identify predictive markers of ESCC immunotherapy.

Follicle-stimulating hormone accelerates osteoclast migration by enhancing methyltransferase-like 3-mediated m6A methylation of cathepsin K.

Follicle-stimulating hormone (FSH) accelerates osteoporosis in postmenopausal women, while the underlying mechanism remains uncharacterized. N6-methyladenosine (m6A) is one of the most important regulations in the development of osteoporosis. In this study, we aimed to investigate the role of FSH in m6A modification and osteoclast function.

Electrochemical Synthesis and Electrocatalytic Oxygen-Evolution Performance of Two-Dimensional NiCo-BPDC Materials.

Metal-organic frameworks (MOFs) have been widely studied as electrocatalysts, and the research strategy to improve their electrocatalytic oxygen evolution reaction (OER) performance is to modify their structure. In this paper, two-dimensional bimetallic MOFs were constructed to improve electrocatalytic OER performance. Using a mild electrochemical method with Ni and Co as metal sources and 4, 4 '-biphenyl dicarboxylic acid (HBPDC) as ligand, two-dimensional NiCo-BPDC was synthesized and then deposited on a carbon cloth electrode.

Progenitor-like exhausted SPRY1CD8 T cells potentiate responsiveness to neoadjuvant PD-1 blockade in esophageal squamous cell carcinoma.

Neoadjuvant immune checkpoint blockade (ICB) demonstrates promise in operable esophageal squamous cell carcinoma (ESCC), but lacks available efficacy biomarkers. Here, we perform single-cell RNA-sequencing of tumors from patients with ESCC undergoing neoadjuvant ICB, revealing a subset of exhausted CD8 T cells expressing SPRY1 (CD8 Tex-SPRY1) that displays a progenitor exhausted T cell (Tpex) phenotype and correlates with complete response to ICB. We validate CD8 Tex-SPRY1 cells as an ICB-specific predictor of improved response and survival using independent ICB-/non-ICB cohorts and demonstrate that expression of SPRY1 in CD8 T cells enforces Tpex phenotype and enhances ICB efficacy.

Mediastinal high-grade vasculogenic mesenchymal tumour with seminoma: a case report and literature review.

Germ cell tumours with somatic-type solid malignancy (GCT-STM) are a rare disease of the mediastinum. Recently, a cohort of vasculogenic mesenchymal tumour (VMT)-nonseminoma cases with different prognoses were recognized and reported. Here, we report a case of mediastinal high-grade VMT with a seminoma.

Analysis of the Clinicopathological Characteristics, Prognosis, and Lymphocyte Infiltration of Esophageal Neuroendocrine Neoplasms: A Surgery-Based Cohort and Propensity-Score Matching Study.

Background: Esophageal neuroendocrine neoplasms (E-NENs) are a rare and poorly reported subtype of esophageal carcinoma. We analyzed the differences in clinicopathological features, prognosis, and tumor-infiltrating lymphocytes (TILs) between E-NENs and esophageal squamous cell carcinoma (ESCC).

Methods: A total of 3620 patients who underwent esophagectomy were enrolled retrospectively.

Distinct mutational features across preinvasive and invasive subtypes identified through comprehensive profiling of surgically resected lung adenocarcinoma.

Lung adenocarcinoma (LUAD) is a heterogeneous disease. Our study aimed to understand the unique molecular features of preinvasive to invasive LUAD subtypes. We retrospectively analyzed the clinical, histopathological, and molecular data of 3,254 Chinese patients with preinvasive lesions (n = 252), minimally invasive adenocarcinomas (n = 479), and invasive LUAD (n = 2,523).

Continuous and Rapid Synthesis of UiO-67 by Electrochemical Methods for the Electrochemical Detection of Hydroquinone.

Continuous and rapid synthesis of UiO-67 under mild conditions has been achieved by electrochemical methods for the first time. In the reaction system, a zirconium sheet was utilized as electrodes and a metal source for the assembly of UiO-67. High-crystalline UiO-67 with a regular tetrahedral morphology of around 1 μm was obtained within 1.

Rapid and Low-Cost Electrochemical Synthesis of UiO-66-NH with Enhanced Fluorescence Detection Performance.

Rapid and low-cost synthesis of metal-organic frameworks (MOFs) are very meaningful for their future practical application. In the present study, a Zr-based ultrastable MOF, UiO-66-NH, was successfully synthesized by electrochemical method using metal Zr as the metal source at room temperature and atmospheric pressure. The effects of the reaction conditions, including the ratio of solvent (electrolyte), the applied voltage and different reaction time, on the crystallinity, morphology, and synthesis rate of the product were fully investigated.

Rapid and Large-Scale Synthesis of IRMOF-3 by Electrochemistry Method with Enhanced Fluorescence Detection Performance for TNP.

Rapid and large-scale synthesis of metal-organic frameworks (MOFs) materials is of great significance for their practical applications. For the first time, we have electrochemically synthesized IRMOF-3 at room temperature by applying a voltage to a zinc electrode immersed in electrolyte containing 2-aminoterephthalic acid (NH-HBDC). The reaction conditions, including the ratio of solvent (electrolyte), the applied voltage, and different reaction times, were investigated and optimized.

Follicular Stimulating Hormone Accelerates Atherogenesis by Increasing Endothelial VCAM-1 Expression.

Postmenopausal atherosclerosis (AS) has for decades been attributed to estrogen deficiency. Although the follicular stimulating hormone (FSH) levels rise sharply in parallel, the direct effect of FSH on AS has never been investigated. In this study, we explored the possible role of FSH in the development of AS.

Postsynthetic Modification of ZIF-90 for Potential Targeted Codelivery of Two Anticancer Drugs.

Combination therapy has been regarded as a promising strategy for cancer treatment due to the enhanced anticancer efficacy achieved by blocking multiple drug resistance pathways. In this work, a drug carrier based on nanoscale ZIF-90 for the codelivery of two anticancer drugs has been synthesized by covalently attaching doxorubicin (DOX) to the surface of ZIF-90 via Schiff base reaction of amino group in DOX and aldehyde group of imidazole-2-carboxaldehyde (ICA) ligand and encapsulating 5-fluorouracil (5-FU) into the pores of the framework. The results of drug loading measurements show that the loading amount of drugs was estimated as high as 36.

Testosterone promotes tube formation of endothelial cells isolated from veins via activation of Smad1 protein.

Testosterone (T) deficiency is positively correlated with the increased incidence of cardiovascular disease. However, the effects of T on vascular endothelial cells remain obscure. Tube formation capacity is critical for vascular regeneration/repair and Smad1 plays an important role in these events.

17β-Estradiol Enhances Vascular Endothelial Ets-1/miR-126-3p Expression: The Possible Mechanism for Attenuation of Atherosclerosis.

Context: Endothelial microRNA 126 (miR-126) attenuates the development of atherosclerosis (AS). However, there is no evidence showing the role of miR-126 in estrogen's antiatherogenic effects.

Objective: We hypothesized that 17β-estradiol (E2) modulates miR-126 expression and thus may improve endothelial function and retard AS development.

Combined technology for clomazone herbicide wastewater treatment: three-dimensional packed-bed electrochemical oxidation and biological contact degradation.

The clomazone herbicide wastewater was treated using a combined technology composed of electrochemical catalytic oxidation and biological contact degradation. A new type of electrochemical reactor was fabricated and a Ti/SnO2 electrode was chosen as the anode in electrochemical-oxidation reactor and stainless steel as the cathode. Ceramic rings loaded with SnO2 were used as three-dimensional electrodes forming a packed bed.

Effectiveness and pathways of electrochemical degradation of pretilachlor herbicides.

Pretilachlor used as one kind of acetanilide herbicides is potentially dangerous and biorefractory. In this work, electrochemical degradation of lab-synthetic pretilachlor wastewater was carried out with Sb doped Ti/SnO(2) electrode as anode and stainless steel as cathode. The effect of current density on pretilachlor degradation was investigated, and the degradation pathway of pretilachlor was inferred by analyzing its main degradation intermediates.