The Mortimer-Filkins test of problem drinking: a review of psychometric properties.

This paper contains a literature review of the psychometric properties of the Mortimer-Filkins test of problem drinking. The test was devised initially to identify problem drinkers from among drink-driving offenders. It was initially validated against a general population, and has since been used with worksite populations.

Localization of the gene for human proliferating nuclear antigen/cyclin by in situ hybridization.

Proliferating cell nuclear antigen (PCNA)/cyclin has been localized by in situ hybridization to the short arm of human chromosome 20 with a peak of grains over band 20p13. In addition, there were two strong secondary peaks of grains over 11p15.1 and Xp11.

Mapping of class alpha glutathione S-transferase 2 (GST-2) genes to the vicinity of the d locus on mouse chromosome 9.

Recombinant inbred strains of mice were used to localize the genes coding for the class alpha glutathione S-transferase 2 (Gst-2). The genes showed three distinct strain distribution patterns, indicating that they occur in at least three clusters separable by recombination. All three clusters are located in the vicinity of the d locus on mouse chromosome 9, but two of them are closer to d than the third.

Genetic variability at the human tumor necrosis factor loci.

Variability in the structure of the human tumor necrosis factor (TNF-alpha) or lymphotoxin (TNF-beta) genes may contribute to the functional polymorphism of the HLA gene complex. We have characterized an allelic restriction fragment length polymorphism (RFLP) of the TNF-beta gene by using the restriction endonuclease NcoI. Digestion of genomic DNA with NcoI and Southern blotting by using TNF-alpha gene probes show 5.

Drug research: a comparison of ongoing research and perceived research priorities.

Since drug abuse results in high costs to the community, it is vital that research funding be allocated in a way which ensures a maximum improvement in health for the money spent. This study explores the extent to which current research into drug and alcohol abuse in Australia reflects perceived research priorities. Two hundred and seventy-four ongoing research projects into drug and alcohol misuse were identified.

Comparison of a quantity-frequency method and a diary method of measuring alcohol consumption.

This study compared the results obtained with two measures of alcohol consumption, a quantity-frequency (QF) method and a retrospective diary method, using a worksite sample. In order to control for the possibility of order effects, a subsample was chosen for readministration of the alcohol measures, when the order of presentation of the two measures was reversed for half of the subsample. Varying the order of presentation of the alcohol consumption items did not significantly affect the results obtained, except for an interaction effect between drinking group and order of presentation.

The prevalence and sociodemographic correlates of high-risk and problem drinking at an industrial worksite.

The study of 833 employees aimed to determine the prevalence and sociodemographic correlates of high-risk and problem drinking in an industrial population. Variables measured included stressful life events, neuroticism, job satisfaction, years of service, job classification and type of shift. As measured by a 7-day retrospective diary, 12.

Emergency department deaths.

This study reviews 186 deaths resulting from trauma in a 2-year period in the Charity Hospital of Louisiana at New Orleans Accident Room in order to evaluate problems in prehospital and hospital resuscitative care. All subjects underwent autopsy, and only six were found to have injuries compatible with survival. Three of these were late arrivals (by transfer or self-imposed delay) and died of protracted hemorrhage.

Localization of the human UbB polyubiquitin gene to chromosome band 17p11.1-17p12.

The chromosomal location of the human ubiquitin genes has been evaluated by in situ hybridization. Because of the conservation of the ubiquitin sequence, coding-region probes cannot distinguish between specific ubiquitin genes and reveal ubiquitin sequences in a number of different chromosomal regions. The major sites of hybridization with a coding-region probe include 17p11.

Dispersion population models discrete in time and continuous in space.

We analyze a discrete-time model of populations that grow and disperse in separate phases. The growth phase is a nonlinear process that allows for the effects of local crowding. The dispersion phase is a linear process that distributes the population throughout its spatial habitat.

Mapping the gene for murine T-cell growth factor, Il-2, to bands B-C on chromosome 3 and for the alpha chain of the IL2-receptor, Il-2ra, to bands A2-A3 on chromosome 2.

Il-2, the gene coding for the cytokine IL2 in the mouse, has been localized by in situ hybridization to bands 3B or 3C on Chromosome 3, where it joins five other genes that also map to 4q in humans. The Il-2 probe also produces a small, secondary grain peak on Chromosome 11, in the vicinity of band 11A5, the significance of which has not yet been determined. The gene for the alpha chain of the IL2 receptor in the mouse, Il-2ra, localizes to bands 2A2 or 2A3 on Chromosome 2.

A nonlinear structured population model of tumor growth with quiescence.

A nonlinear structured cell population model of tumor growth is considered. The model distinguishes between two types of cells within the tumor: proliferating and quiescent. Within each class the behavior of individual cells depends on cell size, whereas the probabilities of becoming quiescent and returning to the proliferative cycle are in addition controlled by total tumor size.

A critical review of Australian cancer organizations' public education material.

Because of the potential benefits of primary prevention and early detection of cancer, a considerable proportion of the efforts of State cancer organizations has been directed towards public educational programs. The study aimed to determine the level of agreement in the messages contained in the written educational material of the State and Territory cancer organizations in Australia. Pamphlets and brochures dealing with primary and secondary prevention of breast, cervical, skin and bowel cancers were obtained.

Molecular genetics of the human glutathione S-transferase.

Multiple human cytosolic glutathione transferases have been described. These enzymes are the products of multiple genes that can be classified into at least four evolutionary classes. The genes encoding each class appear to be clustered on distinct chromosomes.

Genetic heterogeneity of the human glutathione transferases: a complex of gene families.

The glutathione transferases (GSTs) are involved in the metabolism of a wide range of compounds of both exogenous and endogenous origin. There is evidence that deficiency of GST may increase sensitivity to certain environmentally derived carcinogens. In contrast, elevated expression has been implicated in resistance to therapeutic drugs.

A molecular modelling study of the interaction of noradrenaline with the beta 2-adrenergic receptor.

A model of the beta 2-adrenergic receptor binding site is built from the primary structure of the receptor, experimental evidence for key binding residues and analogy with a homologous protein of partially determined structure. It is suggested that residues Trp-109, Thr-110 and Asp-113 are involved in ligand binding. Noradrenaline is successfully docked into this model, and the results of an INDO molecular orbital calculation on the complex indicate that a charge transfer interaction between Trp-109 and noradrenaline is possible.

An approach to the teaching of host/parasite population modelling.

Computer-aided learning is considered to be useful in the teaching of host/parasite population modelling. The STELLA programme for the Apple Macintosh microcomputer allows dynamic models to be developed by drawing a diagram of the interactions and then defining the relationships between variables in terms of simple mathematics or as graphs. Our experience in using this programme for project-based teaching is described.

Isolation of a cDNA clone and localization of the human glutathione S-transferase 3 genes to chromosome bands 11q13 and 12q13-14.

A partial cDNA clone of the glutathione S-transferase 3 gene (GST3) was obtained by screening a lambda gt11 human lung cDNA library with antiserum to human lung GST3. The sequence of this cDNA showed two base differences from the coding sequence of a GST3 cDNA isolated from a human placental cDNA library. Hybridization of the cloned GST3 cDNA to human chromosomes resulted in a primary peak of grains over band 11q13, a localization predicted by prior experiments.

Fragile X testing in a diagnostic cytogenetics laboratory.

Chromosome results obtained from 1012 patients referred with developmental delay without known cause within the three years 1985 to 1987 are reported. G banding analysis and assessment of 70 cells for fragile X gave abnormal results in 84 cases: fragile X in 31 patients and other abnormalities in 53 patients. A further 16 sibs expressing the fragile X were detected in family studies originating from the 31 index cases.

Diagnostic aids for the detection of urine in the equine ejaculate.

An experiment was conducted to evaluate three commercially available test kits, the Azostix, Multistix and Uric-acid test, for the detection of urine in the equine ejaculate. Azostix, which tests for urea nitrogen, consistently detected urine in the equine ejaculate. Urine contamination was evident when a color change occurred in the reagent pad, going from yellow to green after 10 sec of exposure.

Structure of the 5' flanking region of the gene encoding human parathyroid-hormone-related protein (PTHrP).

We have characterized a human genomic clone that contains the 5' coding and 5' flanking sequences of the human parathyroid hormone-related protein gene (PTHrP). The 5' end of the gene contains three exons separated by two small introns of 60 and 165 bp, respectively. The coding region of the PTHrP gene exhibits significant structural homology to the human parathyroid hormone gene (PTH), including the position of at least two introns.

Non C-banding variants in some normal families might be homogeneously staining regions.

Three families are reported showing transmission of a previously described variant, which is not associated with any clinical abnormality. The variant involves additional material at the band 9p12, which shows homogeneous staining of intermediate density with GTL- and RBG-banding, and negative staining with CBG-banding. The region stains positively with Feulgen stain.

Filtering effects in reporting work injuries.

Research efforts in the field of occupational injuries have been hampered by difficulties in ascertaining true prevalence rates. Lack of accuracy in reporting and documentation of work injuries leads to loss of information and lack of comparability of work injury statistics. The paper explores some of the factors related to loss of information about work injuries and proposes a descriptive model of the information processing system.

Molecular organization of the cytokine gene cluster, involving the human IL-3, IL-4, IL-5, and GM-CSF genes, on human chromosome 5.

The human genes for the hematopoietic growth factors interleukin-3 (IL-3), IL-5, and granulocyte-macrophage colony-stimulating factor (GM-CSF) have been mapped to 5q23-31. We present in situ hybridization evidence that the human IL-4 gene is located at 5q23.3-31.