[Correlation between Gene SNP and MTX Resistance in Children with ALL].

Objective: To investigate the correlation between single-nucleotide polymorphism (SNP) of gene and resistance to methotrexate (MTX) in children with acute lymphoblastic leukemia (ALL).

Methods: A total of 144 children with ALL who were treated in General Hospital of Ningxia Medical University from January 2015 to November 2021 were enrolled and divided into MTX resistant group and non-MTX resistant group, with 72 cases in each group. Matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS) technology was used to measure the SNP of gene in all children and analyze its correlation with MTX resistant.

Community Fecal Carriage and Molecular Epidemiology of Extended-Spectrum β-Lactamase- and Carbapenemase-Producing from Healthy Children in the Central South China.

Background: Fecal carriage of extended-spectrum β-lactamase-producing (ESBL-EC) and carbapenemase-producing (CP-EC) is well reported among hospitalized adults and children. However, there are few studies on the carriage prevalence and ESBL-EC and CP-EC genotypes among healthy children in China.

Patients And Methods: Stool samples were collected from 330 students in 2021 from three randomly selected primary schools in Changsha, China.

Fructose-coated Ångstrom silver prevents sepsis by killing bacteria and attenuating bacterial toxin-induced injuries.

Serious infection caused by multi-drug-resistant bacteria is a major threat to human health. Bacteria can invade the host tissue and produce various toxins to damage or kill host cells, which may induce life-threatening sepsis. Here, we aimed to explore whether fructose-coated Ångstrom-scale silver particles (F-AgÅPs), which were prepared by our self-developed evaporation-condensation system and optimized coating approach, could kill bacteria and sequester bacterial toxins to attenuate fatal bacterial infections.

Comparative Evaluation of Seven Tigecycline Susceptibility Testing Methods for Carbapenem-Resistant .

Purpose: Carbapenem-resistant Enterobacteriaceae (CRE) strains are extensively resistant to most antibiotics. Tigecycline is one of the few effective drugs that can be used to treat infections caused by CRE. The aim of this study was to evaluate the accuracy of different methods for detecting the susceptibility of CRE to tigecycline.

[Analysis of pathogen spectrum and antimicrobial resistance of pathogens associated with hospital-acquired infections collected from 11 teaching hospitals in 2018].

To investigate the spectrum and antimicrobial resistance of major pathogens causing nosocomial infections in China, 2018. Non-duplicated nosocomial cases as well as pathogens causing bloodstream infections (BSI), hospital-acquired pneumonia (HAP) and intra-abdominal infections (IAI) from 11 teaching hospitals across China were collected. The minimum inhibitory concentrations (MICs) of clinically significant strains were determined by agar dilution method or broth microdilution method.

Ångstrom-scale silver particle-embedded carbomer gel promotes wound healing by inhibiting bacterial colonization and inflammation.

Poor wound healing after diabetes or extensive burn remains a challenging problem. Recently, we presented a physical approach to fabricate ultrasmall silver particles from Ångstrom scale to nanoscale and determined the antitumor efficacy of Ångstrom-scale silver particles (AgÅPs) in the smallest size range. Here we used the medium-sized AgÅPs (65.

Quasiparticle Lifetime of the Repulsive Fermi Polaron.

We investigate the metastable repulsive branch of a mobile impurity coupled to a degenerate Fermi gas via short-range interactions. We show that the quasiparticle lifetime of this repulsive Fermi polaron can be experimentally probed by driving Rabi oscillations between weakly and strongly interacting impurity states. Using a time-dependent variational approach, we find that we can accurately model the impurity Rabi oscillations that were recently measured for repulsive Fermi polarons in both two and three dimensions.

Stabilization of the Closed-Ring Isomer of Spiropyran by Amide Naphthotube in Water and Its Application in Naked-Eye Detection of Toxic Paraoxon.

The thermodynamically unstable, colourless closed-ring isomer of spiropyran can be stabilized in water by the anti-configurational isomer of amide naphthotube. The influence of the binding on the thermodynamics and kinetics of spiropyran have been studied. The complex was further used to prepare a test paper that allows naked-eye detection of toxic paraoxon.

Radio-Frequency Response and Contact of Impurities in a Quantum Gas.

We investigate the radio-frequency spectroscopy of impurities interacting with a quantum gas at finite temperature. In the limit of a single impurity, we show using Fermi's golden rule that introducing (or injecting) an impurity into the medium is equivalent to ejecting an impurity that is initially interacting with the medium, since the "injection" and "ejection" spectral responses are simply related to each other by an exponential function of frequency. Thus, the full spectral information for the quantum impurity is contained in the injection spectral response, which can be determined using a range of theoretical methods, including variational approaches.

Risk Factors for Subsequential Carbapenem-Resistant Clinical Infection Among Rectal Carriers with Carbapenem-Resistant .

Purpose: Carbapenem-resistant (CRKP) infection has become a critical clinical concern for its high mortality. Rectal carriage of CRKP has been reported playing an important role in CRKP infection; however, the extent to which carrier develops clinical CRKP infection is unclear. This study aimed to identify risk factors for developing subsequential CRKP clinical infection in rectal carriers with CRKP.

A retrospective study on Escherichia coli bacteremia in immunocompromised patients: Microbiological features, clinical characteristics, and risk factors for shock and death.

Background: To evaluate clinical features, bacterial characteristics, and risk factors for shock and mortality of immunocompromised patients with Escherichia coli bacteremia.

Methods: A nearly 6-year retrospective study of E coli bacteremia in 188 immunocompromised patients at Xiangya Hospital was conducted. Demographic, clinical, and laboratory data were documented.

Clinical characteristics and risk factors for shock and death from E. coli bacteremia in pediatric hematological patients.

Introduction: The aim of our study was to evaluate the epidemiology, clinical features and risk factors for shock and mortality from Escherichia coli bacteremia among children and adolescents with hematological disorders.

Methodology: A retrospective observational study of E. coli bacteremia in the hematology department at Xiangya Hospital from January 2013 to June 2018 was conducted.

Fecal Carriage and Epidemiology of Carbapenem-Resistant Among Hospitalized Patients in a University Hospital.

Purpose: To determine the prevalence and epidemiology of fecal carriage of carbapenem-resistant (CRE), antimicrobial susceptibility, carbapenemase phenotype/genotype, and the colistin-resistance gene in a university hospital in China.

Methods: A comprehensive study of the fecal carriage of CRE in 704 patients was performed. Multilocus sequence typing (MLST) and pulsed-field gel electrophoresis (PFGE) were applied to elucidate the molecular epidemiology of the isolates.

O25b-ST131 and O16-ST131 causing urinary tract infection in women in Changsha, China: molecular epidemiology and clinical characteristics.

Objective: This study aimed to investigate the prevalence of ST131 and molecularly characterize the O25b-ST131 and O16-ST131 subgroups among urinary tract infection (UTI) isolates from women in central China. We also assessed the clinical characteristics and outcomes of infections caused by ST131.

Methods: Between January 2014 and December 2015, a total of 216 consecutive, non-repetitive isolates were recovered from UTI urine samples from women in Changsha, China.

Epidemiology and molecular characterization of in recovered from patients with bloodstream infections in Changsha, central China.

Objectives: The main aim of this study was to investigate the prevalence and molecular characteristics of the gene in isolates obtained from all patients with bloodstream infections over a year in a Chinese teaching hospital. We also assessed the susceptibility profiles of the -positive strains and prognostic impact of this gene on the patients.

Methods: A total of 144 consecutive, non-repetitive isolates causing bloodstream infections were collected at a teaching hospital in Changsha, China from January to December 2016.

Molecular recognition of organophosphorus compounds in water and inhibition of their toxicity to acetylcholinesterase.

Molecular tubes with hydrogen bonding donors in their deep hydrophobic cavities are able to selectively bind organophosphorus compounds in water through hydrogen bonding and the hydrophobic effect. They can also be used as a fluorescent sensor for nerve agent simulants and as an inhibitor to reduce the toxicity of paraoxon to acetylcholinesterase.

Variational Approach for Impurity Dynamics at Finite Temperature.

We present a general variational principle for the dynamics of impurity particles immersed in a quantum-mechanical medium. By working within the Heisenberg picture and constructing approximate time-dependent impurity operators, we can take the medium to be in any mixed state, such as a thermal state. Our variational method is consistent with all conservation laws and, in certain cases, it is equivalent to a finite-temperature Green's function approach.

Enhanced His@AuNCs oxidase-like activity by reduced graphene oxide and its application for colorimetric and electrochemical detection of nitrite.

Enzyme-mimicking (nanozyme)-based biosensors are attractive owing to their unique catalytic efficiency, multifunctionality, and tunable activity, but examples of oxidase-like nanozymes are quite rare. Herein, we demonstrated that histidine-capped gold nanoclusters (His@AuNCs) possessed intrinsic oxidase-like activity, which could directly oxidize 3,3',5,5'-tetramethylbenzidine (TMB) to blue colored ox-TMB without HO. The assembly of reduced graphene oxide (RGO) with His@AuNCs could further improve its oxidase-like activity and the His@AuNCs/RGO nanocomposites had a lower Michaelis constant (K) and higher catalytic constant (K) for TMB oxidation.

Fluorescent monitoring of the reaction kinetics of nonfluorescent molecules enabled by a fluorescent receptor.

A facile fluorescent method was developed to quantitatively monitor the hydrolysis kinetics of nonfluorescent esters by using a fluorecent endo-functionalized molecular tube and its recognition ability towards small polar molecules in water. It is possible to determine the apparent rate constants and study the structure-activity relationship.

Characterization of clinical extensively drug resistant Pseudomonas aeruginosa from a Chinese teaching hospital.

Introduction: Pseudomonas aeruginosa, an important opportunistic pathogen, carries multiple virulence factors which contribute to its adaptation and pathogenicity. The goal of this study was to characterize the virulence factors among extensively drug-resistant P. aeruginosa.

Weak Localization and Antilocalization in Topological Materials with Impurity Spin-Orbit Interactions.

Topological materials have attracted considerable experimental and theoretical attention. They exhibit strong spin-orbit coupling both in the band structure (intrinsic) and in the impurity potentials (extrinsic), although the latter is often neglected. In this work, we discuss weak localization and antilocalization of massless Dirac fermions in topological insulators and massive Dirac fermions in Weyl semimetal thin films, taking into account both intrinsic and extrinsic spin-orbit interactions.

[Emergence of methicillin-resistant Staphylococcus aureus SCCmec type IV/V epidemic clones in a large teaching hospital in China].

Objective: To investigate the staphylococcal cassette chromosome (SCC) genotype and molecular epidemiological characteristics of healthcare-associated methicillin-resistant (HA-MRSA) in a large teaching hospital in China.

Methods: From January 2012 to December 2012, a total of 71 nonduplicate HA-MRSA were collected in a teaching hospital in Changsha, China. SCC types were determined by multiplex PCR, and Panton-Valentine leukocidin (PVL) gene was detected by PCR.

Molecular Recognition and Chirality Sensing of Epoxides in Water Using Endo-Functionalized Molecular Tubes.

Chiral epoxides are important intermediates in chemistry and biology. The high-throughput screening of asymmetric epoxidation conditions requires fast determination of the absolute configurations and ee values of chiral epoxides. Herein, we report molecular recognition and chiroptical sensing of epoxides in water using endo-functionalized molecular tubes.