Minimizing therapeutically induced anemia.

A model for erythroid production based on a continuous maturation-proliferation scheme is developed. The model includes a simple control mechanism operating at the proliferating cell level, and analytic solutions for the time dependent response of the model are derived. Using this model, the response of the erythron to a massive depletion of the proliferating cell compartment (due for example to cytostatic drugs or radiation) is calculated.

Analysis of different variants of erythrocyte survival using digital computers.

In the paper a method is presented, enabling parameter estimation of erythrocyte survival patterns, what is an important element in the diagnosis of haemolytic anaemias. The method is based on a computer program, fitting the experimental data to eight variants of theoretical patterns with use of a weighted least squares criterion. A simple statistical test of approximation quality is described and several interpretational examples are given.

Congenital non-spherocytic haemolytic anaemia variants with primary and secondary pyruvate kinase deficiency. II. Enzymatic studies.

Some metabolic effects associated with defective pyruvate kinase (PK) in two variants of congenital non-spherocytic haemolytic anaemia with primary PK and primary adenosine triphosphatase (ATP-ase) (Mg++) deficiency respectively we compared. In one patient with a low erythrocyte ATP level, decreased PK activity appeared together with the irreversible loss of its sensitivity to fructose-I,6-diphosphate (FDP), independently of the experimental conditions. In the second patient, the decrease in PK activity associated with an elevated erythrocyte ATP level was a secondary effect, due to primary ATP-ase (Mg++) deficiency.

Congenital non-spherocytic haemolytic anaemia variants with primary and secondary pyruvate kinase deficiency. I. Erythrokinetic patterns.

The patterns of survival of isotope-labelled erythrocytes were examined in patients suffering from two variants of congenital non-spherocytic haemolytic anaemia with decreased erythrocyte pyruvate kinase (PK) activity. In one variant, with primary PK defect (PPKD) random destruction of erythrocytes was predominant in the process of haemolysis. In the second variant, with primary magnesium activated adenosine triphosphatase (ATP-ase) (Mg++) deficiency and a secondary decrease in PK activity, erythrocytes were destroyed by senescence.

Immunostimulatory effect of zinc in patients with acute lymphoblastic leukemia.

The studies have been performed in 27 ALL patients, aged 4 to 21 years, during their first remission induced by the Memphis Center therapeutic schedule (New Eng. J. Med.

[Effect of calf thymus extracts on the surface structure and the lysosomal apparatus of lymphocytes in patients with chronic lymphatic leukemia].

In 6 patients with chronic lymphatic leukaemia receiving subcutaneously purified calf thymus extract a greater frequency of the following processes was observed: 1) increase in the number of smooth-surface lymphocytes (T cells) in scanning electron microscopy, 2) increased number of lymphocytes forming rosettes with uncoated sheep erythrocytes, 3) increased number of lymphocytes showing high activity of acid phosphatase in cytochemical investigations.

Enzymatic pattern of glucose metabolic pathways in pyruvate kinase-deficient erythrocytes.

It has been shown that in some cases of congenital non-spherocytic haemolytic anaemia (CNSHA) with pyruvate kinase deficiency, the primary defect may be related to diminished magnesium-stimulated ATPase activity, followed by elevation of the erythrocyte ATP level. ATP as the end product of glycolysis inhibits by negative feedback control the activities of key glycolytic enzymes involved in energy production, i.e.