Spontaneous tumors of the prostate gland in inbred strains of mice.

Spontaneous tumors of the prostate gland in the house mouse (Mus musculus) have rarely been described. In a histologic survey of prostate glands from old (greater than 700 days of age) mice, we have observed 2 primary adenocarcinomas of the prostate gland, 1 in a 1,047-day-old C57BL/6J mouse (DEH 1009) and 1 with metastases to the kidney and lungs in a 778-day-old C57BL/KsJ mouse (DEH 705C). We have also found a rhabdomyosarcoma of the prostate gland in a C57BL/6J mouse (DEH 1121C) 732 days old.

Intradermal transplantation in mice of small numbers of sarcoma cells followed by tumor growth and regression.

Relatively small numbers of Sarcoma 1 cells were transplanted intradermally in A/WySn mice to study the course of growth when the size of the transplant is comparable to an early stage of spontaneous cancer. Transplants of 31 to 32,000 cells took one of three courses: no apparent growth; development of tumor followed by regression; or progressive growth. Transplants of 8,000 cells were the most evenly divided among these three courses and were selected for further study.

Culture of cells in beem capsules: a new technique for electron microscopic study of monolayer cultures.

A method is described for the monolayer cultivation of primary cell suspensions and established cell lines directly in carbon-coated BEEM capsules, BEEM capsules are routinely employed by electron microscopists in tissue embedding procedures; growing monolayer cultures directly on the lids of inverted BEEM capsules presents the obvious advantage of maintaining cell to cell and cell to substratum conthaets with a minimum of stress and damage in the preparative steps for electron microscopy.