Synaptic-like coupling of macrophages to myofibers regulates muscle repair.

Peripheral injury responses essential for muscle repair and nociception require complex interactions of target tissues, immune cells and primary sensory neurons. Nociceptors and myofibers both react robustly to signals generated from circulating immune cells, which promote repair, growth, and regeneration of muscle while simultaneously modulating peripheral sensitization. Here, we found that macrophages form a synaptic-like contact with myofibers to hasten repair after acute incision injury and to facilitate regeneration after major muscle damage.

Obesity uncovers presence of inflammatory lung macrophage subsets with adipose tissue transcriptomic signature in influenza virus infection.

Obesity is a risk factor for increased lung damage and disease severity during influenza virus infection. White adipose tissue (WAT) inflammation is a key driver of disease pathogenesis in obesity. Whether and how obesity modifies lung and WAT immune cell character and function in obesity to amplify influenza disease severity remains unknown.

Protocol for cytokine and uterine immune cell characterization in a mouse model of LPS-induced preterm birth.

Inflammation-driven preterm birth (PTB) is modeled in mice using lipopolysaccharide (LPS) challenge. Here, we present a protocol for cytokine and uterine immune cell characterization in a mouse model of LPS-induced PTB. We describe steps for LPS challenge, in vivo cytokine capture assay, and isolation of uterine immune cells for flow cytometry.

BAFF and APRIL counterregulate susceptibility to inflammation-induced preterm birth.

Clinical evidence points to a function for B cell-activating factor (BAFF) in pregnancy. However, direct roles for BAFF-axis members in pregnancy have not been examined. Here, via utility of genetically modified mice, we report that BAFF promotes inflammatory responsiveness and increases susceptibility to inflammation-induced preterm birth (PTB).

Cardiomyocyte Deletion of Exacerbates QT- and RR-Interval Prolongation in Mice.

Circadian rhythms are generated by cell autonomous circadian clocks that perform a ubiquitous cellular time-keeping function and cell type-specific functions important for normal physiology. Studies show inducing the deletion of the core circadian clock transcription factor in adult mouse cardiomyocytes disrupts cardiac circadian clock function, cardiac ion channel expression, slows heart rate, and prolongs the QT-interval at slow heart rates. This study determined how inducing the deletion of in adult cardiomyocytes impacted the electrophysiological phenotype of a knock-in mouse model for the arrhythmogenic long QT syndrome ( ).

Circadian clocks regulate cardiac arrhythmia susceptibility, repolarization, and ion channels.

Daily changes in the incidence of sudden cardiac death (SCD) reveal an interaction between environmental rhythms and internal circadian rhythms. Circadian rhythms are physiological rhythms that alter physiology to anticipate daily changes in the environment. They reflect coordinated activity of cellular circadian clocks that exist throughout the body.

Pulse pressure findings following upper cervical care: a practice-based observational study.

Introduction: Pulse pressure is an indicator of cardiovascular health and is the difference between systolic and diastolic blood pressures. An important etiologic consideration is autonomic nervous system balance. The purpose of this study is to observe pulse pressure changes following a six-week course of care utilizing an upper cervical technique.

Predicting the relative efficacy of shock waveforms for transthoracic defibrillation in dogs.

Study Objective: Previous work has shown that a passive membrane model using a parallel resistor-capacitor circuit is capable of predicting optimal waveforms for transvenous defibrillation. This study tested the ability of that model to predict optimal waveforms for transthoracic defibrillation.

Methods: This study was divided into 3 parts, each of which determined transthoracic defibrillation thresholds (DFTs) in 6 dogs for several different waveform shapes and durations.

Transmembrane potential changes caused by monophasic and biphasic shocks.

Transmembrane potential change (DeltaVm) during shocks was recorded by a double-barrel microelectrode in 12 isolated guinea pig papillary muscles. After 10 S1 stimuli, square-wave S2 shocks of both polarities were given consisting of 10-ms monophasic and 10/10-ms and 5/5-ms biphasic waveforms that created potential gradients from 1.1 +/- 0.

African American teen mothers' perceptions of parenting.

The purpose of this study was to describe the childbearing African American teens' perceptions of parenting based on their own experiences. Focus group discussions were held with 17 teens in their school setting for 50 minutes each week. Group discussions were audiotaped, tapes were transcribed, and then analyzed for common themes.

Maternal-fetal attachment and perceived relationships with important others in adolescents.

We examined associations between maternal-fetal attachment and the pregnant adolescent's perceived relationships with her mother and the baby's father. Questionnaires were administered in health department prenatal clinics to a sample of 61 Mexican-American, African-American, and Caucasian adolescents at 13 to 40 weeks' gestation. Each woman complete Cranley's maternal-fetal attachment scale (MFAS), an investigator-developed relationship with mother scale and a survey instrument that assessed living situation, perceptions of the relationship with the baby's father, and standard demographic variables.

Conversion of the beckman liquid phase sequencer to a gas-liquid phase sequencer.

As an effective aid to extend the microsequencing capabilities the Beckman protein/peptide sequenator Series 890C has been successfully converted to a gas-liquid system, in which coupling buffer 25% trimethylamine was employed as a gas, and heptafluorobutyric acid as a liquid. The system has been found to be efficient for microsequencing (less than 100 pmol). The details of mechanical, plumbing, and other minor changes are described in this paper along with the results of sequencing proteins and peptides, directly and from blots.

Complement-induced granulocyte aggregation in vivo.

Previous studies from our laboratories have demonstrated that granulocytes (PMNs), when exposed to activated complement (C) (specifically C5a), will aggregate and be provoked to damage cultured endothelial cells in vitro; it was postulated that these phenomena might also occur in vivo, constituting a previously unsuspected mechanism of immune tissue damage. The studies here presented confirm by intravital microscopy that PMN aggregation and leukoembolization in fact occur in live animals when C is activated or C5a is infused, and that these are accompanied by extravasation of plasma proteins in a pattern suggesting endothelial damage. It is concluded that altered microvascular behavior of PMNs is a possible pathogenetic mechanism in disease states associated with C activation.

Factors Affecting the Control of Rotylenchulus reniformis with Electromagnetic Energy.

The reniform nematode Rotylenchulus reniformis was reduced in the upper 10 cm of soil with application of UHF electromagnetic energy. Bioassay of treated soil indicated no delayed effect on the population from the treatment. The population was significantly reduced by hot water treatments at 40 C for 10 min, and at 45 C for 5 and 10 min, 50 C and above killed all nematodes.