Protocol for predicting host-microbe interactions and their downstream effect on host cells using MicrobioLink.

Analyzing host-microbe interactions is essential for understanding how microbiota changes disrupt host homeostasis. Here, we present a protocol for predicting host-microbe protein-protein interactions and their downstream effects using MicrobioLink. We describe steps for setting up the environment, installing software, and preparing human transcriptomic and bacterial proteomic data.

Early prediction of preeclampsia using the first trimester vaginal microbiome.

Preeclampsia is a severe obstetrical syndrome which contributes to 10-15% of all maternal deaths. Although the mechanisms underlying systemic damage in preeclampsia-such as impaired placentation, endothelial dysfunction, and immune dysregulation-are well studied, the initial triggers of the condition remain largely unknown. Furthermore, although the pathogenesis of preeclampsia begins early in pregnancy, there are no early diagnostics for this life-threatening syndrome, which is typically diagnosed much later, after systemic damage has already manifested.

KDM5A/B contribute to HIV-1 latent infection and survival of HIV-1 infected cells.

Combinational antiretroviral therapy (cART) suppresses human immunodeficiency virus type 1 (HIV-1) viral replication and pathogenesis in acquired immunodeficiency syndrome (AIDS) patients. However, HIV-1 remains in the latent stage of infection by suppressing viral transcription, which hinders an HIV-1 cure. One approach for an HIV-1 cure is the "shock and kill" strategy.

A comparison of outcomes between class-II and class-III obese patients undergoing robotic ventral hernia repair: a multicenter study.

Background: Morbid obesity has been considered a contraindication to ventral hernia repair (VHR) in the past. However, the relationship between a greater body mass index (BMI) and adverse outcomes has yet to be established in the minimally invasive sphere, particularly with robotics, which may offer an effective surgical option in these high-risk patients. We sought to investigate this relationship by comparing the outcomes of class-II (BMI: 35-39.

Examining the Associations Between Childhood Trauma, Resilience, and Depression: A Multivariate Meta-Analysis.

Topic Of Review: Childhood trauma has been associated with increased depression; however, resilience has been found to reduce this association.

Method Of Review: Present analyses were based on multivariate meta-analytical techniques, an extension of univariate meta-analysis. All computations were performed using the metafor package and the metaRmat package from R.

Health Outcomes Following Childhood Maltreatment: An Examination of the Biopsychosocial Model.

Child maltreatment constitutes a notoriously large problem within the healthcare field and is associated with significant biological, psychological, and social consequences across the lifespan. The primary aim of this study was to examine the biopsychosocial outcomes of an adult population who reported a history of child maltreatment. Data from the Midlife Development in the United States 2 Biomarker Project were used.

'I had to tell my GP I had lung cancer': patient perspectives of hospital- and community-based lung cancer care.

Lung cancer care spans both hospital- and community-based healthcare settings, and suboptimal communication between healthcare providers impacts on continuity and quality of care. Patients' experiences regarding: (1) communication between healthcare providers; and (2) the role of their GP during cancer treatment was explored in interviews with 47 Western Australian lung cancer patients. Thematic analysis using a phenomenological approach was undertaken to derive key themes regarding participant experiences.

Feasibility, tolerability and safety of pediatric hyperpolarized Xe magnetic resonance imaging in healthy volunteers and children with cystic fibrosis.

Background: Hyperpolarized Xe is a promising contrast agent for MRI of pediatric lung function, but its safety and tolerability in children have not been rigorously assessed.

Objective: To assess the feasibility, safety and tolerability of hyperpolarized Xe gas as an inhaled contrast agent for pediatric pulmonary MRI in healthy control subjects and in children with cystic fibrosis.

Materials And Methods: Seventeen healthy control subjects (ages 6-15 years, 11 boys) and 11 children with cystic fibrosis (ages 8-16 years, 4 boys) underwent Xe MRI, receiving up to three doses of Xe gas prepared by either a commercially available or a homebuilt Xe polarizer.

Literacy for health: an interdisciplinary model.

Traditionally, many literacy and health education programs have had difficulty in significantly affecting vulnerable priority populations. The materials used were largely generalized for one language, one level of literacy, and one culture. A multidiscipline review of literature discusses the relationship between literacy, health, and culture and provides rationale for the interdisciplinary literacy for health model.

Diagnosis of adult polycystic kidney disease by genetic markers and ultrasonographic imaging in a voluntary family register.

Diagnosis of autosomal dominant adult polycystic kidney disease (APKD) is possible by ultrasonographic scanning (USS) or by using DNA markers linked to the PKD1 locus. Ultrasonography is complicated by the age dependent penetrance of the gene and linkage studies are subject to recombination errors owing to meiotic crossing over and locus heterogeneity. This study draws on data collected from a voluntary family register of APKD over 10 years.

Adult polycystic kidney disease: knowledge, experience, and attitudes to prenatal diagnosis.

One hundred and ninety subjects from 100 adult polycystic kidney disease (APKD) families on the North Western Regional Genetic Register were interviewed to determine the likely demand for prenatal diagnosis. A detailed questionnaire was used to assess understanding and experience of clinical, therapeutic, and genetic aspects of APKD. Major features of the disease (presence of renal cysts which can lead to renal failure) and forms of therapy (dialysis and transplantation) were known; knowledge of less common features was related to experience.

Absence of linkage between adult polycystic kidney disease and the major histocompatibility system.

Four multiplex families affected with adult polycystic kidney disease were investigated for segregation of the disease with haplotypes bearing HLA-A and B antigens and Bf allotypes. In no case did the disease travel with specific haplotypes within families showing an absence of linkage between the disease and the major histocompatability system. In addition, the disease was not associated with any HLA-A or B antigen or Bf allotype either within or among the families studied.

Acute orbital cellulitis.

A review of 104 patients with acute orbital cellulitis during the past decade showed that the frequency of hospital admissions for this disease has increased recently. Roentgenograms showed paranasal sinus in 77 of 91 patients. Haemophilus influenzae and Diplococcus pneumoniae were recovered from the blood of 20 and 6 patients, respectively.