Comparative Physiology and Morphology of BLA-Projecting NBM/SI Cholinergic Neurons in Mouse and Macaque.

Cholinergic projection neurons of the nucleus basalis and substantia innominata (NBM/SI) densely innervate the basolateral amygdala (BLA) and have been shown to contribute to the encoding of fundamental and life-threatening experiences. Given the vital importance of these circuits in the acquisition and retention of memories that are essential for survival in a changing environment, it is not surprising that the basic anatomical organization of the NBM/SI is well conserved across animal classes as diverse as teleost and mammal. What is not known is the extent to which the physiology and morphology of NBM/SI neurons have also been conserved.

Comparative physiology and morphology of BLA-projecting NBM/SI cholinergic neurons in mouse and macaque.

Cholinergic projection neurons of the nucleus basalis and substantia innominata (NBM/SI) densely innervate the basolateral amygdala (BLA) and have been shown to contribute to the encoding of fundamental and life-threatening experiences. Given the vital importance of these circuits in the acquisition and retention of memories that are essential for survival in a changing environment, it is not surprising that the basic anatomical organization of the NBM/SI is well conserved across animal classes as diverse as teleost and mammal. What is not known is the extent to which the physiology and morphology of NBM/SI neurons have also been conserved.

A cobalt(II) coordination polymer-derived catalyst engineered temperature-induced semi-reversible single-crystal-to-single-crystal (SCSC) dehydration for efficient liquid-phase epoxidation of olefins.

Single-crystal-to-single-crystal (SCSC) transformations provide more avenues for phase transitions, which have piqued great interest in crystal engineering. In this work, a 3D Co(II)-based coordination polymer (CP), (1), (where (btec) = 1,2,4,5-benzenetetracarboxylate) undergoes SCSC transition upon heating at 180 °C to afford an anhydrous phase (1'). Room-temperature water-vapour induced semi-reversible SCSC transformation of 1' involves condensation of two water molecules coordinating to the metal cluster, yielding a new framework (2).

Analysis of laboratory data transmission between two healthcare institutions using a widely used point-to-point health information exchange platform: a case report.

Objective: The objective was to identify information loss that could affect clinical care in laboratory data transmission between 2 health care institutions via a Health Information Exchange platform.

Materials And Methods: Data transmission results of 9 laboratory tests, including LOINC codes, were compared in the following: between sending and receiving electronic health record (EHR) systems, the individual Health Level Seven International (HL7) Version 2 messages across the instrument, laboratory information system, and sending EHR.

Results: Loss of information for similar tests indicated the following potential patient safety issues: (1) consistently missing specimen source; (2) lack of reporting of analytical technique or instrument platform; (3) inconsistent units and reference ranges; (4) discordant LOINC code use; and (5) increased complexity with multiple HL7 versions.

Enhanced Catalytic Activity of a Copper(II) Metal-Organic Framework Constructed Semireversible Single-Crystal-to-Single-Crystal Dehydration.

Herein, we present a copper(II) metal-organic framework, () [(btec) = 1,2,4,5-benzenetetracarboxylate], that undergoes single-crystal-to-single-crystal transformations into two anhydrous phases and with the chemical formula , triggered by two-step dehydration at 403 and 433 K, respectively. After immersion in water for 3 days at room temperature, transformed into (), while both and took 1 week to revert to . Dynamic vapor sorption studies validated water-induced reversible structural transformations at 70% relative humidity (RH).

30 years of [C]methyl triflate.

Some scientific discoveries are well known only to a core group of researchers working on technical subjects. Nevertheless, they open new research directions, allow existing knowledge to be viewed in entirely new and useful ways, or provide a way to make something that was hard or impossible to make before. Carbon-11 methyl triflate ([C]MeOTf) is one such advance, facilitating the synthesis of many carbon-11 radio tracers and broadening the range of applications of carbon-11 radiochemistry.

Supporting Equity and Inclusion of Deaf and Hard-of-Hearing Individuals in Professional Organizations.

Disability is an important and often overlooked component of diversity. Individuals with disabilities bring a rare perspective to science, technology, engineering, mathematics, and medicine (STEMM) because of their unique experiences approaching complex issues related to health and disability, navigating the healthcare system, creatively solving problems unfamiliar to many individuals without disabilities, managing time and resources that are limited by physical or mental constraints, and advocating for themselves and others in the disabled community. Yet, individuals with disabilities are underrepresented in STEMM.

First prospective clinical trial of reduced incision bariatric procedures using magnetic liver retraction.

Background: Exposure of the surgical field is an essential component of minimally invasive surgery. Liver retraction is an important element of bariatric procedures because visualization of the stomach and gastroesophageal junction is key. The magnetic surgical system provides a well-tolerated and effective option for adjustable liver retraction without the use of a dedicated port.

Variation in CT use for paediatric head injuries across different types of emergency departments in Australia and New Zealand.

Objectives: CT of the brain (CTB) for paediatric head injury is used less frequently at tertiary paediatric emergency departments (EDs) in Australia and New Zealand than in North America. In preparation for release of a national head injury guideline and given the high variation in CTB use found in North America, we aimed to assess variation in CTB use for paediatric head injury across hospitals types.

Methods: Multicentre retrospective review of presentations to tertiary, urban/suburban and regional/rural EDs in Australia and New Zealand in 2016.

Exploring intermolecular contacts in multi-substituted benzaldehyde derivatives: X-ray, Hirshfeld surface and lattice energy analyses.

Crystal structures of six benzaldehyde derivatives (1-6) have been determined and their supramolecular networks were established by an X-ray crystallographic study. The study has shown that the compounds are linked by various intermolecular interactions such as weak C-H⋯O hydrogen bonding, and C-H⋯π, π-π and halogen bonding interactions which consolidate and strengthen the formation of these molecular assemblies. The carbonyl group generates diverse synthons in 1-6 intermolecular C-H⋯O hydrogen bonds.

Research capacity of Australian and New Zealand emergency medicine departments.

Background: Large, multicentre studies are required in emergency medicine to advance clinical care and improve patient outcomes. The Australasian College for Emergency Medicine clinical trials network is available to researchers to assist with facilitating large, multicentre research. However, there is no current information about the research capacity of emergency departments (EDs) in Australia and New Zealand.

Prediction of Individual Cochlear Implant Recipient Speech Perception With the Output Signal to Noise Ratio Metric.

Objectives: A cochlear implant (CI) implements a variety of sound processing algorithms that seek to improve speech intelligibility. Typically, only a small number of parameter combinations are evaluated with recipients but the optimal configuration may differ for individuals. The present study evaluates a novel methodology which uses the output signal to noise ratio (OSNR) to predict complete psychometric functions that relate speech recognition to signal to noise ratio for individual CI recipients.

Intrinsic Excitability Increase in Cerebellar Purkinje Cells after Delay Eye-Blink Conditioning in Mice.

Cerebellar-based learning is thought to rely on synaptic plasticity, particularly at synaptic inputs to Purkinje cells. Recently, however, other complementary mechanisms have been identified. Intrinsic plasticity is one such mechanism, and depends in part on the downregulation of calcium-dependent SK-type K channels, which contribute to a medium-slow afterhyperpolarization (AHP) after spike bursts, regulating membrane excitability.

An Investigation of Audibility Effects on Cochlear Implant Speech Perception Prediction.

Output Signal to Noise Ratio (OSNR) is the Signal to Noise Ratio (SNR) at the output of a cochlear implant (CI) sound processor. Whereas other prediction metrics typically predict mean speech-in-noise test scores for a group of subjects, an OSNR-based model has been shown to accurately predict scores for individual CI recipients. The OSNR model was unable to predict scores for aggressive Ideal Binary Mask (IdBM) sound processing.

An Investigation of the Effect of AGC Gain on the Output Signal to Noise Ratio in Cochlear Implant Sound Processing.

Measurement of speech intelligibility of cochlear implant (CI) recipients is typically carried out with a speech-innoise test procedure. Metrics which predict speech intelligibility can pre-screen new sound processing strategies prior to comprehensive testing with human subjects.The Output Signal to Noise Ratio (OSNR) metric calculates the Signal to Noise Ratio (SNR) which is present at the CI sound processor output.

Optimizing the radiosynthesis of [Ga]DOTA-MLN6907 peptide containing three disulfide cyclization bonds - a GCC specific chelate for clinical radiopharmaceuticals.

In the present study, the effect of radiolabeling conditions on radiolabeling efficiency and achievable specific activity of a DOTA-conjugated highly-lipophilic peptide containing three disulfide cyclization bonds was examined. The peptide is designed to bind specifically (with high affinity) to cell-surface receptor guanylyl cyclase C (GCC), which is universally expressed by colorectal cancer cells. The effect of systematic variation of chemical parameters pH, mass of peptide, acetate buffer concentration (ionic strength), and inclusion of ethanol in the radiolabeling reaction vessel on achievable specific activity and labeling efficiency was examined.

Y-DOTATOC Dosimetry-Based Personalized Peptide Receptor Radionuclide Therapy.

Pretherapy PET with Y-DOTATOC is considered the ideal dosimetry protocol for Y-DOTATOC therapy; however, its cost, limited availability, and need for infusion of amino acids to mimic the therapy administration limit its use in the clinical setting. The goal of this study was to develop a dosimetric method for Y-DOTATOC using Y-DOTATOC PET/CT and bremsstrahlung SPECT/CT and to determine whether dosimetry-based administered activities differ significantly from standard administered activities. This was a prospective phase 2 trial of Y-DOTATOC therapy in patients with somatostatin receptor-positive tumors.

An Evaluation of Output Signal to Noise Ratio as a Predictor of Cochlear Implant Speech Intelligibility.

Objectives: Cochlear implant (CI) sound processing strategies are usually evaluated in clinical studies involving experienced implant recipients. Metrics which estimate the capacity to perceive speech for a given set of audio and processing conditions provide an alternative means to assess the effectiveness of processing strategies. The aim of this research was to assess the ability of the output signal to noise ratio (OSNR) to accurately predict speech perception.

LAGOS-NE: a multi-scaled geospatial and temporal database of lake ecological context and water quality for thousands of US lakes.

Understanding the factors that affect water quality and the ecological services provided by freshwater ecosystems is an urgent global environmental issue. Predicting how water quality will respond to global changes not only requires water quality data, but also information about the ecological context of individual water bodies across broad spatial extents. Because lake water quality is usually sampled in limited geographic regions, often for limited time periods, assessing the environmental controls of water quality requires compilation of many data sets across broad regions and across time into an integrated database.

RNA-Seq Analysis of Differentiated Keratinocytes Reveals a Massive Response to Late Events during Human Papillomavirus 16 Infection, Including Loss of Epithelial Barrier Function.

The human papillomavirus (HPV) replication cycle is tightly linked to epithelial cell differentiation. To examine HPV-associated changes in the keratinocyte transcriptome, RNAs isolated from undifferentiated and differentiated cell populations of normal, spontaneously immortalized keratinocytes (NIKS) and NIKS stably transfected with HPV16 episomal genomes (NIKS16) were compared using next-generation sequencing (RNA-Seq). HPV16 infection altered expression of 2,862 cellular genes.

Correction: RESPECT-ED: Rates of Pulmonary Emboli (PE) and Sub-Segmental PE with Modern Computed Tomographic Pulmonary Angiograms in Emergency Departments: A Multi-Center Observational Study Finds Significant Yield Variation, Uncorrelated with Use or Small PE Rates.

[This corrects the article DOI: 10.1371/journal.pone.

A simulation analysis of the variability of the roving level hearing test.

In the study of auditory prostheses, the Speech Recognition Threshold (SRT) is the Signal to Noise Ratio (SNR) at which 50% of words are correctly identified. SRT is typically measured using an adaptive procedure wherein speech is presented at a fixed sound pressure level (SPL) and the noise level is varied according to the subject's responses. A roving level SRT test has been used by researchers with the goal of including the effectiveness of Automatic Gain Control (AGC) systems in SRT measurements.

Localization of Unknown Primary Site with Ga-DOTATOC PET/CT in Patients with Metastatic Neuroendocrine Tumor.

Localization of the site of the unknown primary tumor is critical for surgical treatment of patients presenting with neuroendocrine tumor (NET) with metastases. Forty patients with metastatic NET and unknown primary site underwent Ga-DOTATOC PET/CT in a single-site prospective study. The Ga-DOTATOC PET/CT was considered true-positive if the positive primary site was confirmed by histology or follow-up imaging.

RESPECT-ED: Rates of Pulmonary Emboli (PE) and Sub-Segmental PE with Modern Computed Tomographic Pulmonary Angiograms in Emergency Departments: A Multi-Center Observational Study Finds Significant Yield Variation, Uncorrelated with Use or Small PE Rates.

Introduction: Overuse of CT Pulmonary Angiograms (CTPA) for diagnosing pulmonary embolism (PE), particularly in Emergency Departments (ED), is considered problematic. Marked variations in positive CTPA rates are reported, with American 4-10% yields driving most concerns. Higher resolution CTPA may increase sub-segmental PE (SSPE) diagnoses, which may be up to 40% false positive.