Genetic Dissection of Growth and Eco-Physiological Traits Associated with Altitudinal Adaptation in Sakhalin Fir () Based on QTL Mapping.

(1) Background: The genetic basis of local adaptation in conifers remains poorly understood because of limited research evidence and the lack of suitable genetic materials. Sakhalin fir () is an ideal organism for elucidating the genetic basis of local adaptation because its altitudinal adaptation has been demonstrated, and suitable materials for its linkage mapping are available. (2) Method: We constructed P336 and P236 linkage maps based on 486 and 516 single nucleotide polymorphisms, respectively, that were derived from double digest restriction site-associated DNA sequences.

Temperature-related cline in the root mass fraction in East Asian wild radish along the Japanese archipelago.

Wild plants with a wide distribution, including those exposed to a wide variety of environmental conditions, may have variations in key functional traits relevant for agricultural applications. The East Asian wild radish ( var. ) is an appropriate model plant because it is widely distributed and has outstanding sink capacity as well as two cultivars within the species.

TodoFirGene: Developing Transcriptome Resources for Genetic Analysis of Abies sachalinensis.

Todo-matsu (Abies sachalinensis) is one of the most important forestry species in Hokkaido, Japan and is distributed from near sea level to the alpine zone. Due to its wide spatial distribution, the species adapts to its environment, displaying phenotypes of ecological relevance. In order to identify candidate genes under natural selection, we collected the transcriptome from the female and male flower, leaf and inner bark.

Influence of low- and high-elevation plant genomes on the regulation of autumn cold acclimation in Abies sachalinensis.

Boreal coniferous species with wide geographic distributions show substantial variation in autumn cold acclimation among populations. To determine how this variation is inherited across generations, we conducted a progeny test and examined the development of cold hardening in open-pollinated second-generation (F2) progeny of Abies sachalinensis. The F1 parents had different genetic backgrounds resulting from reciprocal interpopulational crosses between low-elevation (L) and high-elevation (H) populations: L × L, L × H, H × L, and H × H.

Development of 32 EST-SSR markers for Abies firma (Pinaceae) and their transferability to related species.

Premise Of The Study: We developed simple sequence repeat (SSR) markers from expressed sequence tags (ESTs) for Abies firma, a conifer endemic in Japan, to facilitate evaluation of the population genetic structure in this species. •

Methods And Results: We designed primers for 153 EST-SSRs identified from 486322 ESTs from A. sachalinensis ESTs, and tested 96 of them for PCR amplification.

Modeling intraspecific adaptation of Abies sachalinensis to local altitude and responses to global warming, based on a 36-year reciprocal transplant experiment.

Intraspecific adaptation in Abies sachalinensis was examined using models based on long-term monitoring data gathered during a reciprocal transplant experiment with eight seed source populations and six transplantation sites along an altitudinal gradient. The consequence of local adaptation was evaluated by testing the home-site advantage for upslope and downslope transplants at five ages. The populations' fitness-linked trait was set as their productivity (tree height × survival rate) at each age.