Immunoreactivity of proteolytic degradation products of human prostatic acid phosphatase.

Prostatic acid phosphatase (EC 3.1.3.

Stabilization of human prostate acid phosphatase by cross-linking with diimidoesters.

1. Modification of dimeric human prostate acid phosphatase (EC 3.1.

Detection of prostatic tissue and seminal plasma peptides reacting with human seminal fluid acid phosphatase antibodies.

IgG1 monoclonal antibody to purified seminal fluid phosphatase was raised by fusion of spleen cells from immunized mice with cell line Sp2/O-Ag 14 using simple method of screening for antiphosphatase antibody secreting clones. All molecular forms of catalytically active seminal fluid phosphatase and prostatic tissue phosphatase, resolved by chromatofocusing in pH gradient, react with this monoclonal antibody and with rabbit antiserum to purified seminal fluid phosphatase. Peptides of Mr 25,000 to 76,000 and of Mr 13,000 to 76,000 were adsorbed from the prostatic tissue extract and from seminal plasma on the monoclonal antibody-Sepharose column.

Phosphotyrosine as a substrate of acid and alkaline phosphatases.

A new spectrophotometric method for following dephosphorylation of phosphotyrosine has been described. The absorption spectra of phosphotyrosine and tyrosine were plotted over the pH range from 3 to 9. The change in absorbance accompanying the conversion of phosphotyrosine to tyrosine was the greatest at 286 nm.

Phosphoprotein phosphatase activity of human prostate acid phosphatase.

Human prostate acid phosphatase (EC 3.1.3.