Publications by authors named "Wasimul B Chowdhury"

Vibrio parahaemolyticus is responsible for seafood-related gastroenteritis worldwide. In Bangladesh, diarrhea is endemic and diarrheagenic V. parahaemolyticus serotypes occur naturally in the coastal and estuarine aquatic environment.

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Forty-two strains of Vibrio parahaemolyticus were isolated from Bay of Bengal estuaries and, with two clinical strains, analyzed for virulence, phenotypic, and molecular traits. Serological analysis indicated O8, O3, O1, and K21 to be the major O and K serogroups, respectively, and O8:K21, O1:KUT, and O3:KUT to be predominant. The K antigen(s) was untypeable, and pandemic serogroup O3:K6 was not detected.

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Article Synopsis
  • A species-specific RNA colony blot hybridization protocol was created to effectively identify and count Vibrio cholerae and Vibrio mimicus in environmental water samples, showcasing its ability to accurately target these bacteria.
  • The method demonstrated 100% sensitivity for the targeted species and 90% specificity for nontarget organisms in laboratory tests, proving reliable with no false results in treated lake water samples.
  • This protocol was successfully applied to real-world pond water samples in cholera-endemic areas of Bangladesh, revealing patterns in V. cholerae populations that preceded clinical cholera cases, thus underscoring its potential for environmental monitoring.
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