S-Locus F-Box Proteins Are Solely Responsible for S-RNase-Based Self-Incompatibility of Pollen.

Self-incompatibility (SI) in is regulated by a polymorphic S-locus. For each S-haplotype, the S-locus contains a pistil-specific gene and multiple pollen-specific () genes. Both gain-of-function and loss-of-function experiments have shown that alone regulates pistil specificity in SI.

Use of Domain-Swapping to Identify Candidate Amino Acids Involved in Differential Interactions between Two Allelic Variants of Type-1 S-Locus F-Box Protein and S3-RNase in Petunia inflata.

Petunia inflata possesses a self-incompatibility (SI) mechanism, which involves S-RNase and multiple S-locus F-box (SLF) genes at the polymorphic S-locus. For a given S-haplotype, each SLF is thought to interact with some of its non-self S-RNases, but not with its self S-RNase. In this work, we studied an allelic pair of SLF1, S2-SLF1 and S3-SLF1, which differ in 44 amino acids and show differential interactions with S3-RNase.