Cultivation and survival studies of Neisseria gonorrhoeae in a human diploid cell strain.

Neisseria gonorrhoeae was cultivated in a human diploid cell strain (WI-38). Eighty percent of the cultures contained viable gonococci for at least 4 m at 36°C, as evidenced by subculture to brain heart infusion broth. Monthly subcultures of bacteria could be made to fresh WI-38 cultures for at least 11 monthly passages with a 69% survival rate.

Pig liver pyruvate carboxylase. The reaction pathway for the decarboxylation of oxaloacetate.

1. The reaction pathway for the decarboxylation of oxaloacetate, catalysed by pig liver pyruvate carboxylase, was studied in the presence of saturating concentrations of K(+) and acetyl-CoA. 2.

Pig liver pyruvate carboxylase. The reaction pathway for the carboxylation of pyruvate.

1. The reaction pathway for the carboxylation of pyruvate, catalysed by pig liver pyruvate carboxylase, was studied in the presence of saturating concentrations of K(+) and acetyl-CoA. 2.

Pig liver pyruvate carboxylase. Purification, properties and cation specificity.

1. Pyruvate carboxylase was purified to apparent homogeneity from pig liver mitochondria and shown to be free of all kinetically contaminating enzymes. 2.

Reconstitution of a calcium pump using defined membrane components.

A (Mg(2+) + Ca(2+))ATPase (ATP phosphohydrolase, EC 3.6.1.