Publications by authors named "Warerat Kaewduangduen"
is an important pathogen causing invasive infection associated with a high mortality rate. One mechanism that causes the failure of eradication is an increase in regulatory T cells (Treg), which play a major role in immune suppression and promoting pathogenicity. To date, how induces a Treg response remains unclear.
View Article and Find Full Text PDFPolymeric Particle BAM15 Targeting Macrophages Attenuates the Severity of LPS-Induced Sepsis: A Proof of Concept for Specific Immune Cell-Targeted Therapy.
Pharmaceutics
November 2023
Article Synopsis
- The study investigates how altering energy sources in macrophages can influence their functions, specifically in the context of severe inflammatory diseases like sepsis.
- Researchers found that the mitochondrial uncoupling agent BAM15 significantly affected M1 macrophage polarization without impacting M2 polarization, suggesting a targeted therapeutic potential.
- Using BAM15-loaded particles showed better results in reducing inflammation and liver injury in sepsis models, highlighting the effectiveness of a macrophage-specific delivery system for treating severe inflammatory conditions.
Dendritic cells (DCs) are the most potent antigen-presenting cells that have multifaceted functions in the control of immune activation and tolerance. Hyperresponsiveness and altered tolerogenicity of DCs contribute to the development and pathogenesis of system lupus erythematosus (SLE); therefore, DC-targeted therapies aimed at inducing specific immune tolerance have become of great importance for the treatment of SLE. This study developed a new nanoparticle (NP) containing a biodegradable PDMAEMA-PLGA copolymer for target-oriented delivery to DCs in situ.
View Article and Find Full Text PDFDespite an uncommon condition, the clinical management of phlegmon appendicitis (retention of the intra-abdominal appendiceal abscess) is still controversial, and probiotics might be partly helpful. Then, the retained ligated cecal appendage (without gut obstruction) with or without oral dfa1 (started at 4 days prior to the surgery) was used as a representative model. At 5 days post-surgery, the cecal-ligated mice demonstrated weight loss, soft stool, gut barrier defect (leaky gut using FITC-dextran assay), fecal dysbiosis (increased with reduced bacterial diversity), bacteremia, elevated serum cytokines, and spleen apoptosis without kidney and liver damage.
View Article and Find Full Text PDFAlthough bacteria-free DNA in blood during systemic infection is mainly derived from bacterial death, translocation of the DNA from the gut into the blood circulation (gut translocation) is also possible. Hence, several mouse models with experiments on macrophages were conducted to explore the sources, influences, and impacts of bacteria-free DNA in sepsis. First, bacteria-free DNA and bacteriome in blood were demonstrated in cecal ligation and puncture (CLP) sepsis mice.
View Article and Find Full Text PDFArticle Synopsis
- The study investigates the role of the cGAS DNA receptor in sepsis, comparing cGAS deficient mice to wildtype (WT) mice using two sepsis models: cecal ligation and puncture (CLP) and lipopolysaccharide (LPS) injection.
- Results showed that cGAS mice had less severe sepsis outcomes, with lower mortality and inflammation biomarkers compared to WT mice, indicating that cGAS might contribute to the severity of sepsis.
- In WT macrophages, LPS exposure led to significant mitochondrial damage and proinflammatory responses, which were reduced in cGAS-deficient macrophages, suggesting that cGAS activation by cell-free DNA enhances inflammatory responses during se