Classification of condom lubricants in cyanoacrylate treated fingerprints by desorption electrospray ionization mass spectrometry.

Traces of condom lubricants in fingerprints can be valuable information in cases of sexual assault. Ideally, not only confirmation of the presence of the condom but also determination of the type of condom brand used can be retrieved. Previous studies have shown to be able to retrieve information about the condom brand and type from fingerprints containing lubricants using various analytical techniques.

Amino Acid Profiling from Fingerprints Using Amide Stationary-Phase UPLC-MS.

UPLC-MS is a commonly used technique to first separate complex samples and subsequently quantify molecules of interest. Herein we describe the use of UPLC-MS using an amide stationary phase to quantify non-derivatized amino acids extracted from fingerprints. As detector either a triple-quadrupole MS/MS or a TOF-MS detector was used.

Non-marking Collection of Amino Acids from Fingerprints Using Hydrogels.

The amino acid profile obtained from a fingerprint may provide valuable information on its donor. Unfortunately, the collection of chemicals from the fingerprint is often destructive to the fingerprint ridge detail. Herein we detail the use of cross-linkable solutions of dextran-methacrylate to form hydrogels capable of collecting amino acids from surfaces followed by extraction and quantification with UPLC-MS.

ZraP, the most prominent zinc protein under zinc stress conditions has no direct role in in-vivo zinc tolerance in Escherichia coli.

Escherichia coli ZraP (zinc resistance associated protein) is the major Zn containing soluble protein under Zn stress conditions. ZraP is the accessory protein of a bacterial two-component, Zn sensitive signal transduction system ZraSR. ZraP has also been reported to act as a Zn dependent molecular chaperone.

Collection of amino acids and DNA from fingerprints using hydrogels.

The amino acid profile obtained from a fingerprint may provide valuable information on its donor. For forensic scientists, recovering evidence relating to the amino acid profile of a suspect can potentially be valuable for identification and exclusion purposes. Herein we detail the use of cross-linkable solutions of dextran-methacrylate to form hydrogels capable of collecting amino acids from surfaces followed by extraction and quantification with UPLC-MS.

Membrane potential independent transport of NH in the absence of ammonium permeases in Saccharomyces cerevisiae.

Background: Microbial production of nitrogen containing compounds requires a high uptake flux and assimilation of the N-source (commonly ammonium), which is generally coupled with ATP consumption and negatively influences the product yield. In the industrial workhorse Saccharomyces cerevisiae, ammonium (NH) uptake is facilitated by ammonium permeases (Mep1, Mep2 and Mep3), which transport the NH ion, resulting in ATP expenditure to maintain the intracellular charge balance and pH by proton export using the plasma membrane-bound H-ATPase.

Results: To decrease the ATP costs for nitrogen assimilation, the Mep genes were removed, resulting in a strain unable to uptake the NH ion.