Publications by authors named "Waqas Rafique Ali"
Domestic buffaloes (), known as water buffaloes, play a key role as versatile multipurpose agricultural animals in the Asiatic region. Pakistan, with the second-largest buffalo population in the world, holds a rich domestication history of buffaloes. The overall trends in buffalo production demand the genomic characterization of Pakistani buffalo breeds.
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- Cotton leaf curl disease (CLCuD) severely impacts cotton production in countries like Pakistan, leading to annual losses of around 2 billion USD due to its association with multiple begomoviruses and a specific betasatellite.
- The study developed a loop-mediated isothermal amplification (LAMP) assay to quickly and effectively detect CLCuD, using specifically designed primers for better sensitivity.
- The optimized LAMP assay can detect as low as 22 copies of DNA, and its adaptation for colorimetric and real-time applications enables rapid field detection, potentially reducing economic losses and supporting the economies of affected regions.
Antibiotics are generally applied for treatment or as subtherapeutic agents to overcome diseases caused by pathogenic bacteria including Escherichia coli, Salmonella and Enterococcus species in poultry. However, due to their possible adverse effects on animal health and to maintain food safety, probiotics, prebiotics, and synbiotics have been proposed as alternatives to antibiotic growth promoters (AGPs) in poultry production. In this study, the effects of prebiotics on the augmentation of broiler's indigenous gut microbiology were studied.
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