Revelation of enzyme activity of mutant pyrazinamidases from Mycobacterium tuberculosis upon binding with various metals using quantum mechanical approach.

Pyrazinamide (PZA) is one of the most potent bacteriostatic drug against tuberculosis, a deadliest disease with high mortality and morbidity rate. PZA metabolizes into its active form pyrazinoic acid (POA) with the help of a metalloenzyme, pyrazinamidase (PZase). Mutagenicity and metal substitution in PZase weakens the binding of PZA with PZase and increases the drug resistance in Mycobacterium tuberculosis.

Assessment of viable myocardium by nitrate augmented 99mTc MIBI myocardial perfusion imaging.

Objective: To determine the efficacy of 99mTcMIBI with nitrate administration for the detection of viable myocardium in patients with MI.

Methods: Thirty-five patients (31 men, 4 women; mean age 51.91 +/- 8.