Use of spectroscopic process analytical technology for rapid quality evaluation during preparation of CHO cell culture media.

Media preparation parameters contribute significantly to media quality, cell culture performance, productivity, and product quality. Establishing proper media preparation procedures is critical for ensuring a robust CHO cell culture process. Process analytical technology (PAT) enables unique ways to quantify assessments and improve media quality.

Optimization of adaptation parameters from adhesion cell culture in serum-containing media to suspension in chemically defined media by superlative box design.

A new design of experiments-superlative box design (SBD), was adopted to optimize the adaptation of Chinese hamster ovary cells from adhesion culture to serum-free suspension culture. It is a general trend to use a serum-free medium instead of a serum-containing medium. The advantage of serum-free medium (chemically defended) is that it does not contain unknown components and avoids safety issues.

Effects of different sodium salts and nitrogen sources on the production of 3-hydroxybutyrate and polyhydroxybutyrate by Burkholderia cepacia.

The effects of NaCl, NaSO, NaHPO, and NaCHO on the production of 3-hydroxybutyrate, polyhydroxybutyrate, and by-products by Burkholderia cepacia. Proper addition of NaCHO can significantly promote the production of 3-hydroxybutyric acid and polyhydroxybutyrate. The concentration, productivity, and yield of 3-hydroxybutyrate were increased by 48.

Study on In-Situ Synthesis Process of Ti-Al Intermetallic Compound-Reinforced Al Matrix Composites.

In this study, ball-milled powder of Ti and Al was used to fabricate Ti-Al intermetallic compound-reinforced Al matrix composites by an in-situ reaction in cold-pressing sintering and hot-pressing sintering processes. The detailed microstructure of the Ti-Al intermetallic compound-reinforced Al composite was characterized by optical microscopy (OM), X-ray diffraction (XRD), energy dispersive spectrometry (EDS), and electron backscattered diffraction (EBSD). The results indicate that a typical core-shell-like structure forms in the reinforced particles.