Microbiota-derived succinate promotes enterohaemorrhagic Escherichia coli virulence via lysine succinylation.

Succinate upregulates enterohaemorrhagic Escherichia coli (EHEC) virulence. Lysine succinylation, a post-translational modification, regulates cellular function in eukaryotes but is less characterized in bacteria. We hypothesized that lysine succinylation regulates EHEC virulence.

CobB-mediated deacetylation of the chaperone CesA regulates O157:H7 virulence.

Enterohemorrhagic (EHEC) O157:H7 is a common food-borne pathogen that can cause acute diseases. Lysine acetylation is a post-translational modification (PTM) that occurs in various prokaryotes and is regulated by CobB, the only deacetylase found in bacteria. Here, we demonstrated that CobB plays an important role in the virulence of EHEC O157:H7 and that deletion of significantly decreased the intestinal colonization ability of bacteria.

Antifungal efficacy of scorpion derived peptide K1K8 against Candida albicans in vitro and in vivo.

As an alternative class of antimicrobial agents, antimicrobial peptides (AMPs) have gained significant attention. In this study, K1K8, a scorpion AMP derivative, showed effective activity against Candida albicans including clinically resistant strains. K1K8 killed C.

Strategies adopted by Salmonella to survive in host: a review.

Salmonella, a Gram-negative bacterium that infects humans and animals, causes diseases ranging from gastroenteritis to severe systemic infections. Here, we discuss various strategies used by Salmonella against host cell defenses. Epithelial cell invasion largely depends on a Salmonella pathogenicity island (SPI)-1-encoded type 3 secretion system, a molecular syringe for injecting effector proteins directly into host cells.

Serovar Typhi Induces Host Metabolic Reprogramming to Increase Glucose Availability for Intracellular Replication.

serovar Typhi ( Typhi) is a human-limited intracellular pathogen and the cause of typhoid fever, a severe systemic disease. Pathogen-host interaction at the metabolic level affects the pathogenicity of intracellular pathogens, but it remains unclear how Typhi infection influences host metabolism for its own benefit. Herein, using metabolomics and transcriptomics analyses, combined with in vitro and in vivo infection assays, we investigated metabolic responses in human macrophages during .

R-CenterNet+: Anchor-Free Detector for Ship Detection in SAR Images.

In recent years, the rapid development of Deep Learning (DL) has provided a new method for ship detection in Synthetic Aperture Radar (SAR) images. However, there are still four challenges in this task. (1) The ship targets in SAR images are very sparse.

Anaerobic and Microaerobic Pretreatment for Improving Methane Production From Paper Waste in Anaerobic Digestion.

Having been generated with a tremendous amount annually, paper waste (PW) represents a large proportion in municipal solid waste (MSW) and also a potential source of renewable energy production through the application of anaerobic digestion (AD). However, the recalcitrant lignocellulosic structure poses obstacles to efficient utilization in this way. Recently, anaerobic and microaerobic pretreatment have attracted attention as approaches to overcome the obstacles of biogas production.

YhjC is a novel transcriptional regulator required for Shigella flexneri virulence.

is an intracellular pathogen that primarily infects the human colon and causes shigellosis. virulence relies largely on the type III secretion system (T3SS) and secreted effectors. VirF, the master virulence regulator, is essential for the expression of T3SS-related genes.

Salmonella Typhimurium reprograms macrophage metabolism via T3SS effector SopE2 to promote intracellular replication and virulence.

Salmonella Typhimurium establishes systemic infection by replicating in host macrophages. Here we show that macrophages infected with S. Typhimurium exhibit upregulated glycolysis and decreased serine synthesis, leading to accumulation of glycolytic intermediates.

Downregulation of a novel flagellar synthesis regulator AsiR promotes intracellular replication and systemic pathogenicity of typhimurium.

The intracellular pathogen serovar Typhimurium (. Typhimurium) exploits host macrophage as a crucial survival and replicative niche. To minimize host immune response stimulated by flagellin, the expression of flagellar genes is downregulated during .

Steam explosion pretreatment of rice straw to improve structural carbohydrates anaerobic digestibility for biomethanation.

Effectiveness of steam explosion (SE) pretreatment for deconstructing the complex structural carbohydrates (SC) and lignin recalcitrance properties of rice straw (RS) for conjunctive improvement of biofuel yield and waste valorization was evaluated. This work exhibited successful pretreatment of RS at a different pressure (1.2, 1.

Anaerobic digestion of tobacco stalk: biomethane production performance and kinetic analysis.

Tobacco stalk, a common agricultural waste derived from the harvest of tobacco, caused serious environmental pollution in China. In this study, the performance of biomethane production and characteristics of four varieties of tobacco stalk were investigated for the first time. The results showed that the highest cumulative methane yield of 130.

Anaerobic digestion of lipid-rich swine slaughterhouse waste: Methane production performance, long-chain fatty acids profile and predominant microorganisms.

This study investigated methane production, long-chain fatty acids (LCFAs) profile, and predominant microorganisms in anaerobic digestion (AD) of lipid-rich swine slaughterhouse waste (SSW). The maximum methane yield was 999.2 mL/g VS.

Employing response surface methodology (RSM) to improve methane production from cotton stalk.

China is the largest cotton producer with the cotton output accounting for 25% of the total world's cotton production. A large quantity of cotton stalk (CS) waste is generated which is burned and causes environmental and ecological problems. This study investigated the anaerobic digestibility of CS by focusing on improving the methane yield by applying central composite design of response surface methodology (RSM).

In-situ injection of potassium hydroxide into briquetted wheat straw and meadow grass - Effect on biomethane production.

Alkaline pretreatment of lignocellulosic biomass has been intensively investigated but heavy water usage and environmental pollution from wastewater limits its industrial application. This study presents a pretreatment technique by in-situ injection of potassium hydroxide concentrations ranging from 0.8% to 10% (w/w) into the briquetting process of wheat straw and meadow grass.