Assessment of the clonal growth potential of meibomian gland stem/progenitor cells via clonal analysis.

Purpose: Clonal analysis is a feasible method to evaluate the status of stem/progenitor cells in epidermal or limbus investigations. This study aimed to evaluate the clonal growth potential of meibomian gland (MG) epithelial cells using clonal analysis.

Methods: Mouse and human MG tissues were isolated and cocultured with 3T3 feeder cells.