Vertical ozone formation mechanisms resulting from increased oxidation on the mountainside of Mount Tai, China.

The vertical distribution of ozone (O) within the boundary layer (BL) and its ground-level effects have been extensively studied. However, observational limitations in obtaining high-resolution, real-time data on O and its precursors, especially volatile organic compounds (VOCs), have led to a scarcity of research on O formation sensitivity and mechanisms. Online measurements for O, nitrogen oxides (NO ), and VOCs were made on the mountainside of Mount Tai (∼550 m a.

Development of a broad-spectrum one-step immunoassay for detection of glucocorticoids in milk using magnetosome-based immunomagnetic beads.

Immunomagnetic beads provide novel tools for high-throughput immunoassay techniques. In this study, protein G (PG) was immobilized onto bacterial magentic particles (BMPs) using an additional cysteine residue at the C-terminus. A broad-spectrum monoclonal antibody against glucocorticoids (GCs) was attached to BMPs through PG-Fc interaction, generating BMP-PG-mIgG immunomagentic beads.

Enhanced reductive degradation of chloramphenicol by sulfidated microscale zero-valent iron: Sulfur-induced mechanism, competitive kinetics, and new transformation pathway.

Crystalline iron sulfide (FeS, i.e., FeS or FeS) minerals as sulfur sources were used to prepare the mechanochemically sulfidated microscale zero-valent iron ((FeS+ZVI)).

Identification and engineering on the nonconserved residues of metallo-β-lactamase-type thioesterase to improve the enzymatic activity.

The standalone metallo-β-lactamase-type thioesterase (MβL-TE), belongs to the group V nonreducing polyketide synthase agene cluster, catalyzes the rate-limiting step of product releasing. Our work first investigated on the orthologous MβL-TEs from different origins to determine which nonconserved amino acid residues are important to the hydrolysis efficiency. A series of chimeric MβL-TEs were constructed by fragment swapping and site-directed mutagenesis, in vivo enzymatic assay showed that two nonconserved residues A19 and E75 (numbering in HyTE) were critical to the catalytic performance.