[Molecular study of a case with variant of RHCE*ce allele in haplotype dce resulting in weakened e antigen].

Objective: To explore the Rh genotype for a female with RhD(-) blood type and its molecular basis.

Methods: A 26-year-old female who had attended the outpatient clinic of the First Affiliated Hospital of Zhengzhou University in August 2019 was selected as the study subject. Peripheral blood samples were collected from the proband and her parents for Rh phenotyping with gel card method.

Four new alkaloids from the roots of .

Four new alkaloids (-), together with five known ones (-), were isolated from the bulbs of The structures were determined by analysis of their spectroscopic data and quantum-chemical calculations. All the isolates were tested for their ability to modulate neuronal Ca mobilization in primary cultured neocortical neurons. Compound inhibited spontaneous Ca oscillations at low micromolar concentrations.

The effect of body mass index on quality of life in modified single stoma cutaneous ureterostomy or ileal conduit after radical cystectomy.

Objective: To explore the influence of postoperative body mass index (BMI) change on postoperative quality of life (QOL) in patients undergoing radical cystectomy (RC) plus modified single stoma cutaneous ureterostomy (MSSCU) or ileal conduit (IC).

Methods: Patients were divided into two groups according to different BMI change patterns: patients experiencing an elevated postoperative BMI level, along with a clinically significant increase in their BMI (an increase of more than 10%) were categorized as Group 1, while patients experiencing a decrease postoperative BMI level, along with a clinically significant reduction in their BMI (a decrease of more than 5%) were categorized as Group 2. Spearman correlation analysis was used to examine the correlations between quality-of-life scores and postoperative clinical parameters.

Passive silicon on-chip optic accelerometer for low frequency vibration detection.

In this article, a fiber optic accelerometer based on a Fabry-Perot interferometer is presented and prepared by micromachining on a silicon substrate. The inertial sensing structure of the accelerometer is built by a center block mass and four folded springs with a high thickness-to-width ratio, providing an in-plane resonance vibration. The miniature-sized sensor has large flexibility in structural design and its acceleration response can be predicted by theoretical estimation and finite element simulation.

Miniature tri-axis accelerometer based on fiber optic Fabry-Pérot interferometer.

A fiber optic accelerometer with a high sensitivity, low noise, and compact size is proposed for low-frequency acceleration sensing. The sensor is composed of a 20 mm diameter spherical outer frame and a three-dimensional spring-mass structure as the inertial sensing element. Three Fabry-Pérot interferometers (FPI) are formed between flat fiber facets and cubic mass surfaces to measure the FPI cavity length change caused by acceleration.

All-optical light-induced thermoacoustic spectroscopy for remote and non-contact gas sensing.

All-optical light-induced thermoacoustic spectroscopy (AO-LITS) is reported for the first time for highly sensitive and selective gas sensing, in which a commercial standard quartz tuning fork (QTF) is employed as a photothermal detector. The vibration of the QTF was measured by the highly sensitive fiber-optic Fabry-Pérot (FP) interferometry (FPI) technique, instead of the piezoelectric detection in the conventional LITS. To improve the stability of the sensor system, a compact QTF-based fiber-optic FPI module is fabricated by 3D printing technique and a dual-wavelength demodulation method with the ellipse-fitting differential-cross-multiplication algorithm (DW-EF-DCM) is exploited for the FPI measurement.

Four-wavelength quadrature phase demodulation technique for extrinsic Fabry-Perot interferometric sensors.

In this Letter, we report a four-wavelength quadrature phase demodulation technique for extrinsic Fabry-Perot interferometric (EFPI) sensors and dynamic signals. Four interferometric signals are obtained from four different laser wavelengths. A wavelength interval of four wavelengths is chosen according to the free spectrum range (FSR) of EFPI sensors to generate two groups of anti-phase signals and two groups of orthogonal signals.

Ultra-sensitive ppb-level methane detection based on NIR all-optical photoacoustic spectroscopy by using differential fiber-optic microphones with gold-chromium composite nanomembrane.

In this paper, we propose and experimentally demonstrate an ultra-sensitive all-optical PAS gas sensor, incorporating with a near-infrared (NIR) diode laser, fiber-optic microphones (FOMs) and a double channel differential T-type photoacoustic cell. The FOM is realized by Fabry-Perot interferometry and novel gold-chromium (Au-Cr) composite nanomembranes. To meet the demand of high sensitivity and flat frequency response for the FOMs, the Au-Cr composite diaphragm is deliberately designed and fabricated by E-beam evaporation deposition with 330 nm in thickness and 6.

Large-Dynamic-Range and High-Stability Phase Demodulation Technology for Fiber-Optic Michelson Interferometric Sensors.

A large-dynamic-range and high-stability phase demodulation technology for fiber-optic Michelson interferometric sensors is proposed. This technology utilizes two output signals from a 2 × 2 fiber-optic coupler, the interferometric phase difference of which is π. A linear-fitting trigonometric-identity-transformation differential cross-multiplication (LF-TIT-DCM) algorithm is used to interrogate the phase signal from the two output signals from the coupler.

Sensitivity enhanced fiber optic hydrophone based on an extrinsic Fabry-Perot interferometer for low-frequency underwater acoustic sensing.

A miniaturized fiber optic hydrophone (FOH) based on a composite metal diaphragm with an air back cavity and a high finesse extrinsic Fabry-Perot interferometric (EFPI) scheme for low-frequency underwater acoustic sensing is proposed and experimentally demonstrated in this paper. A composite metal diaphragm is used to improve the stability of the hydrophone. A balance channel is used to equilibrate the hydrostatic pressure and maintain an air cavity, which improves the mechanical sensitivity.

Passive Homodyne Phase Demodulation Technique Based on LF-TIT-DCM Algorithm for Interferometric Sensors.

A passive homodyne phase demodulation technique based on a linear-fitting trigonometric-identity-transformation differential cross-multiplication (LF-TIT-DCM) algorithm is proposed. This technique relies on two interferometric signals whose interferometric phase difference is odd times of π. It is able to demodulate phase signals with a large dynamic range and wide frequency band.

High sensitivity fiber-optic Michelson interferometric low-frequency acoustic sensor based on a gold diaphragm.

A Michelson interferometric fiber-optic acoustic sensor based on a large-area gold diaphragm is proposed in this paper. The Michelson interferometer (MI) based on 3×3 coupler is comprised of two beams that reflected from the gold diaphragm and a cleaved fiber end face. Thickness and diameter of the gold diaphragm are 300 nm and 2.

Phthalideisoquinoline Hemiacetal Alkaloids from That Inhibit Spontaneous Calcium Oscillations, Including Alkyl Derivatives of (+)-Egenine That Are Strikingly Levorotatory.

The new phthalideisoquinoline hemiacetal alkaloids (-) and the known analogues ( and ) were isolated from the bulbs of . The new compounds were characterized by analysis of their NMR spectroscopic data, chemical degradation syntheses, X-ray crystallography, and comparison of experimental and calculated ECD data. All the isolates were screened in vitro for inhibitory activity of spontaneous calcium oscillations in primary cultured neocortical neurons.

Micromachined extrinsic Fabry-Pérot cavity for low-frequency acoustic wave sensing.

In this work we demonstrate a low-frequency acoustic sensor structure based on extrinsic Fabry-Pérot interferometer (EFPI) cavity. The cavity is fabricated through micromachining techniques in a square silicon substrate with 4 mm side length and 400 μm thickness, which gives the sensor relative compact size. In the assembling process of the lead-in fiber, a D-shaped ceramic ferrule is designed to achieve the open cavity structure, which can balance the environmental pressure inside and outside the cavity and thus giving the sensor potentials of resisting strong pressure variations in some harsh application environments.

Alkaloids from Corydalis decumbens suppress neuronal excitability in primary cultures of mouse neocortical neurons.

Eight previously undescribed alkaloids, named corydemine, dihydrocorydemine, corydedine, 8,13-dioxo-14-hydroxytetrahydropalmatine, egenine-α-N-oxide, egenine-β-N-oxide, 7'-O-ethylegenine-α-N-oxide, and 7'-O-ethylegenine-β-N-oxide, together with three known ones, muramine, l-tetrahydropalmatine, and (+)-egenine, were isolated from the bulbs of Corydalis decumbens. Their structures were elucidated by comprehensive spectroscopic analysis and chemical correlation. The isolated compounds were tested for their ability to modulate neuronal excitability in primary cultured neocortical neurons.

The application of novel spindle-like polypyrrole hollow nanocapsules containing Pt nanoparticles in electrocatalysis oxidation of nicotinamide adenine dinucleotide (NADH).

Novel spindle-like polypyrrole hollow nanocapsules containing Pt nanoparticles (Pt NPs/PPy composite hollow nanospindles) were successfully prepared by using beta-akaganeite (β-Fe(3+)O(OH,Cl)) nanospindles as templates and methanoic acid as a reducing agent. The β-Fe(3+)O(OH,Cl) templates can be easily obtained in ethanol/water mixing solution in the presence of thiophene and FeCl(3)·6H(2)O, and after coating by PPy shell, they can be gradually and completely etched during the reduction of H(2)PtCl(6) into Pt nanoparticles (Pt NPs) with the average size of 3.6 nm on spindle-like polypyrrole hollow nanocapsules, which could still keep their integrality of morphologies with the thickness of PPy shell of 18-20 nm.

Constructing carbon-coated Fe₃O₄ microspheres as antiacid and magnetic support for palladium nanoparticles for catalytic applications.

Fe₃O₄ microsphere is a good candidate as support for catalyst because of its unique magnetic property and large surface area. Coating Fe₃O₄ microspheres with other materials can protect them from being dissolved in acid solution or add functional groups on their surface to adsorb catalyst. In this paper, a carbon layer was coated onto Fe₃O₄ microspheres by hydrothermal treatment using polyethylene glycol as the connecting agents between glucose and Fe₃O₄ spheres.

Hydroxycoumarin derivatives: novel and potent α-glucosidase inhibitors.

A novel class of hydroxycoumarin derivatives were found to be potent α-glucosidase inhibitors. Their syntheses were reported and the structure-activity relationship was established. Kinetic enzymatic assays indicated that compound 10 was a slow-binding and noncompetitive inhibitor with a Ki value of 589 nM, while compound 11 was a competitive inhibitor with a Ki value of 4.

Fabrication of Pt/polypyrrole hybrid hollow microspheres and their application in electrochemical biosensing towards hydrogen peroxide.

Pt/polypyrrole (PPy) hybrid hollow microspheres were successfully prepared by wet chemical method via Fe(3)O(4) template and evaluated as electrocatalysts for the reduction of hydrogen peroxide. The as-synthesized products were characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray photoelectron spectra (XPS), X-ray diffraction (XRD), inductive coupled plasma emission spectrum (ICP) and Fourier-transform infrared spectra (FTIR) measurements. The results exhibited that ultra-high-density Pt nanoparticles (NPs) were well deposited on the PPy shell with the mean diameters of around 4.

Accurately tuning the dispersity and size of palladium particles on carbon spheres and using carbon spheres/palladium composite as support for polyaniline in H2O2 electrochemical sensing.

With an accurate control of the dispersity and size of the palladium nanoparticles (Pd NPs), carbon spheres/Pd NPs composite was prepared without any extra reducing agents. In order to fully understand the formation mechanism and find out the best condition for the fabrication of carbon/Pd composite spheres, the effects of temperature, reaction time, pH value, and the weight ratio of PdCl(2) to carbon spheres on the morphology of the final products were investigated. A superior product with small (d = 7.

Synthesis and biological activity of 3-(2, 8, 9-trioxa-aza-1-germatricyclo [3. 3. 3. 0]undecane-1-yl)-hydroxycinnamic acids.

The new germanium compounds of cinnamic acid, RC(6)H(4)CHGe(OCH(2)CH(2))(3)NCH(2)COOH R=H (I), 2-OH (II), 3-OH (III), 4-OH (IV), have been obtained to study anti-tumor activities. Compounds (I-IV) were prepared by the electrophilic addition reaction and nucleophilic substitution reaction. Biological investigation has demonstrated that all compounds (I-IV) are lower toxicity with strongly anti-tumor activity than positive control.

9-Substituted berberine derivatives as G-quadruplex stabilizing ligands in telomeric DNA.

The interaction of berberine and its 9-substituted derivatives with human telomeric DNA d[G(3)(T(2)AG(3))(3)](telo21) has been investigated via CD spectroscopy, fluorescence spectroscopy, PCR-stop assay, competitive dialysis, and telomerase repeat amplification protocol (TRAP) assay. The results indicated that these semisynthesized compounds could induce and stabilize the formation of anti-parallel G-quadruplex of telomeric DNA in the presence or absence of metal cations. Compared with berberine, the 9-substituted derivatives exhibit stronger binding affinity with G-quadruplex and higher inhibitory activity for telomerase.

Large-scale synthesis of tungsten oxide nanofibers by electrospinning.

We show in this communication that large-scale synthesis of orthorhombic WO3 nanofibers can be obtained via a simple electrospinning method. The morphology and the crystal structure are investigated by scanning electron microscopy (SEM), transmission electron microscopy (TEM), FTIR, X-ray diffraction patterns (XRD) and X-ray photoelectron spectra (XPS) spectra. SEM and TEM images showed that the diameter of the obtained WO3 nanofibers is between 100 and 500 nm.

Preparation and characterization of Ag(2)S nanoparticles embedded in polymer fibre matrices by electrospinning.

Polyvinylpyrrolidone (PVP)/Ag(2)S composite fibres were successfully prepared by a facile method called the electrospinning technique. Scanning electron microscopy analysis revealed the fibre morphology of the composite. Transmission electron microscopy showed spherical nanoparticles of the Ag(2)S component with an average particle size of about 15 nm and a good dispersion.