Comprehensive analysis of the transcriptomics and metabolomics reveal the changes induced by nano-selenium and melatonin in Zizyphus jujuba Mill. cv. Huizao.

Background: Jujube (Zizyphus jujuba), belonging to the Rhamnaceae, contains various bioactive components including flavonoids and polysaccharides. In our previous study, jujube quality was improved by application of nano-selenium (Nano-Se) and melatonin; however, little research has been conducted on the induced molecular mechanisms. Zizyphus jujuba cv.

Comprehensive analysis of transcriptomics and metabolomics provides insights into the mechanism by plant growth regulators affect the quality of jujube (Ziziphus jujuba Mill.) fruit.

A comprehensively analysis of the transcriptomics and metabolomics was conducted to investigate the mechanism of plant growth regulators on the quality of jujube fruit. After the application of plant growth regulators, a total of 3097 differentially expressed genes (DEGs) were identified, which were mainly annotated in 123 pathways such as flavonoid biosynthesis, metabolism of alanine, aspartate, and glutamate. In addition, 1091 differential expressed metabolites (DEMs), including 519 up-regulated and 572 down-regulated metabolites, were significantly altered after application of plant growth regulators.

Differential Analysis of Serum Principal Components Treated with Compound Decoction and Related Compounds Based on High-Resolution Mass Spectrometry (HRMS).

Objective: To compare the differences in the serum principal components in ulcerative colitis- (UC-) induced rats, treated with compound decoction, matrine, oxymatrine monomer mixture, and indirubin monomer, and to provide a modern scientific basis for elucidating the clinical efficacy of compound decoction for the treatment of UC.

Methods: The serum samples of rats from each group were obtained after drug administration, and the serum principal components of each group were analyzed by high-resolution mass spectrometry. Agilent Eclipse XDB C18 chromatographic column (100 mm × 2.

Coformer selection based on degradation pathway of drugs: a case study of adefovir dipivoxil-saccharin and adefovir dipivoxil-nicotinamide cocrystals.

Adefovir dipivoxil (AD) is a bis(pivaloyloxymethyl) prodrug of adefovir with chemical stability problem. It undergoes two degradation pathways including hydrolysis and dimerization during storage. Pharmaceutical cocrystallization exhibits a promising approach to enhance aqueous solubility as well as physicochemical stability.

Optimization of preparation and property studies on glycosylated albumin as drug carrier for nanoparticles.

Glycosylated BSA was prepared via Maillard reaction and central composite design (CCD) technique was applied to study the effects of reaction time, temperature, the pH value of the buffer solution on the degree of graft (DG), content of the melanoid (A420) and emulsifying stability (ES) of the resultant product. Fluorescence spectra as well as several other techniques including sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), infrared spectrum (IR) and differential scanning calorimetry (DSC) were employed to study the possible differences between the glycosylated BSA and BSA. Glycosylated albumin possessed distinct properties from BSA and had the great potential to be used as the drug carrier for nanoparticles.