Pre-biodrying treatment enhances lignin-related pathways with phenolic hydroxyls as reactive cores to accelerate humification during composting.

The innovative biodrying-enhanced composting (BEC) process produces highly matured fertilizer within 10 d. To clarify the biodrying-accelerated humification mechanism, structural and molecular variations in humic acid (HA) during BEC were compared to those during 16-d bioaugmented mechanical composting without biodrying. Results showed that BEC produced HA with significantly higher aromaticity and molecular size (p < 0.

Prevalence and risk factors of early postoperative seizures in patients with glioma: A protocol for meta-analysis and systematic review.

Introduction: Early postoperative seizures has been the most common clinical expression in gliomas; however, the incidence and risk factors for early postoperative seizures in gliomas are more controversial. This protocol describes a systematic review and meta-analysis to clarify the prevalence and risk factors of early postoperative seizures in patients with glioma.

Methods And Analysis: Searches will be conducted on CNKI, WanFang, VIP, PubMed, Embase, Cochrane Library databases and Web of Science for the period from database inception to December 31st, 2023.

Upregulation of HLA-II related to LAG-3CD4 T cell infiltration is associated with patient outcome in human glioblastoma.

Glioblastoma (GBM) is the most common malignant diffuse glioma of the brain. Although immunotherapy with immune checkpoint inhibitors (ICIs), such as programmed cell death protein (PD)-1/PD ligand-1 inhibitors, has revolutionized the treatment of several cancers, the clinical benefit in GBM patients has been limited. Lymphocyte-activation gene 3 (LAG-3) binding to human leukocyte antigen-II (HLA-II) plays an essential role in triggering CD4 T cell exhaustion and could interfere with the efficiency of anti-PD-1 treatment; however, the value of LAG-3-HLA-II interactions in ICI immunotherapy for GBM patients has not yet been analyzed.

Prognostic marker CXCL5 in glioblastoma polyformis and its mechanism of immune invasion.

Glioblastoma multiforme (GBM) is the most aggressive brain cancer with a poor prognosis. Therefore, the correlative molecular markers and molecular mechanisms should be explored to assess the occurrence and treatment of glioma.WB and qPCR assays were used to detect the expression of CXCL5 in human GBM tissues.

[Retrospective clinical study of hip replacement in the treatment of traumatic arthritis secondary to acetabular fracture].

Objective: To investigate the clinical effect of total hip replacement (THA) in the treatment of traumatic arthritis secondary to acetabular fracture.

Methods: From October 2019 to June 2022, 15 patients with secondary traumatic arthritis of acetabulum fracture were treated with THA. There were 8 males and 7 females, aged from 40 to 76 years old with an average of (59.

Tracing COVID-19 drugs in the environment: Are we focusing on the right environmental compartment?

The Coronavirus Disease 2019 (COVID-19) pandemic led to over 770 million confirmed cases, straining public healthcare systems and necessitating extensive and prolonged use of synthetic chemical drugs around the globe for medical treatment and symptom relief. Concerns have arisen regarding the massive release of active pharmaceutical ingredients (APIs) and their metabolites into the environment, particularly through domestic sewage. While discussions surrounding this issue have primarily centered on their discharge into aquatic environments, particularly through treated effluent from municipal wastewater treatment plants (WWTPs), one often overlooked aspect is the terrestrial environment as a significant receptor of pharmaceutical-laden waste.

Modeling and analyzing the action process of monoamine hormones in depression: a Petri nets-based intelligent approach.

In contemporary society, the incidence of depression is increasing significantly around the world. At present, most of the treatment methods for depression are psychological counseling and drug therapy. However, this approach does not allow patients to visualize the logic of hormones at the pathological level.

Monocular 3D Human Pose Markerless Systems for Gait Assessment.

Gait analysis plays an important role in the fields of healthcare and sports sciences. Conventional gait analysis relies on costly equipment such as optical motion capture cameras and wearable sensors, some of which require trained assessors for data collection and processing. With the recent developments in computer vision and deep neural networks, using monocular RGB cameras for 3D human pose estimation has shown tremendous promise as a cost-effective and efficient solution for clinical gait analysis.

Mental Health Study from the Perspective of Behavior Preference under the Environment of Internet Media: Based on China's Data Analysis.

To prevent the mental health problems of college preference students effectively, this paper analyzes the influence of college students' internet media use behaviors on their mental health in the information age. We make an empirical study on the above problems by using metrology models under the condition of controlling individual factors. The result shows that the mental health of college students is significantly affected by different internet media use behaviors and shows obvious heterogeneity.

Dynamical Behaviors of a Translating Liquid Crystal Elastomer Fiber in a Linear Temperature Field.

Liquid crystal elastomer (LCE) fiber with a fixed end in an inhomogeneous temperature field is capable of self-oscillating because of coupling between heat transfer and deformation, and the dynamics of a translating LCE fiber in an inhomogeneous temperature field are worth investigating to widen its applications. In this paper, we propose a theoretic constitutive model and the asymptotic relationship of a LCE fiber translating in a linear temperature field and investigate the dynamical behaviors of a corresponding fiber-mass system. In the three cases of the frame at rest, uniform, and accelerating translation, the fiber-mass system can still self-oscillate, which is determined by the combination of the heat-transfer characteristic time, the temperature gradient, and the thermal expansion coefficient.

Sequencing and phylogenetic analysis of the complete chloroplast genome of Blume.

Blume is a perennial medicinal herb widely distributed in China, Korea and Japan. In this study, the complete chloroplast genome sequence of was assembled and characterized based on high-throughput sequencing data. The whole chloroplast genome is 170,610 bp in length and contains 95,485 bp large single-copy (LSC) and 22,605 bp small single-copy (SSC) regions separated by a pair of 26,260 bp inverted repeat (IR) regions.

The complete chloroplast genome of Engler and its phylogenetic analysis in the family Araceae.

Engler is a perennial herbaceous medicinal plant, which is widely distributed in many provinces in China such as Anhui, Jiangsu, and Zhejiang. In this study, the complete chloroplast genome sequence of was assembled and characterized based on high-throughput sequencing data. The total length of chloroplast genome was 175,537 bp, including large single-copy (LSC) and small single-copy (SSC) regions of 98,870 bp and 23,345 bp, respectively, which were separated by a pair of 27,161 bp inverted repeat (IR) regions.

The complete chloroplast genome of wild Rehd. et Wils.

Rehd. et Wils. is an important traditional Chinese herbal medicine and widely distributed in the south of Yangtze River in China.

De novo assembly and Transcriptome Analysis of the Momordica charantia Seedlings Responding to methyl jasmonate using 454 pyrosequencing.

Momordica charantia, a medicinal and edible species of the Cucurbitaceae family, has been widely used as a vegetable around the world. Hundreds of pharmacological compounds isolated from the M. charantia have been reported.

Study on the efficient removal of azo dyes by heterogeneous photo-Fenton process with 3D flower-like layered double hydroxide.

As organic dyes are the main pollutants in water pollution, seeking effective removal solutions is urgent for humans and the environment. A novel environmentally friendly three-dimensional CoFe-LDHs (3D CoFe-LDHs) catalyst was synthesized by one-step hydrothermal method. Scanning electron microscopy, energy dispersive spectroscopy, Fourier transform infrared spectra, X-ray diffraction, X-ray photoelectron spectroscopy, Brunauer-Emmett-Teller technique as well as UV-Vis diffuse reflectance spectra were used to characterize the prepared samples.

Non-Genomic Action of Androgens is Mediated by Rapid Phosphorylation and Regulation of Androgen Receptor Trafficking.

Background: Testosterone is critical for maintaining spermatogenesis and male fertility. The accomplishment of these processes requires the synergistic actions of the classical and non-classical signaling pathways of androgens.

Methods: A murine testicular Sertoli cell line, TM4 cell was used to examine androgen actions in Sertoli cells.

Naturally occurring genetic mutations in the 5'-upstream regulatory region of bovine FSHB generate a novel cis-regulatory element that affects its expression.

We previously reported that numerous naturally occurring genetic mutations in the 5'-upstream regulatory region (5'-URR) of the bovine follicle-stimulating hormone beta-subunit gene (FSHB) were associated with reduced serum follicle-stimulating hormone (FSH) levels, poor-quality semen and low fertility in bulls. In addition, two different FSHB mRNA transcripts resulting from the linked mutations of genomic DNA were discovered in mutation-bearing bull pituitaries. Here, using electrophoretic mobility shift assay, we identified c.

Expression of microRNA‑26b and identification of its target gene EphA2 in pituitary tissues in Yanbian cattle.

microRNAs (miRNAs/miRs) are a class of single‑stranded non‑coding RNA molecules of 19‑24 nucleotides (nt) in length. They are widely expressed in animals, plants, bacteria and viruses. Via specific mRNA complementary pairing of target genes, miRNAs are able to regulate the expression of mRNA levels or inhibit protein translation following transcription.

Enhanced gene transfection efficiency in CD13-positive vascular endothelial cells with targeted poly(lactic acid)-poly(ethylene glycol) nanoparticles through caveolae-mediated endocytosis.

The efficient delivery of therapeutic gene into cells of interest is a critical challenge to broad application of non-viral vectors. The approach of introducing ligands that lead gene vectors to target caveolae-mediated endocytosis on nanoparticle surface might serve as a promising strategy for the effective gene transfection. Recently, in an attempt to enhance the possibility of caveolae-mediated endocytosis, we fabricated a peptide-targeted gene vector for highly efficient receptor-mediated intracellular delivery.

Novel cationic 6-lauroxyhexyl lysinate modified poly(lactic acid)-poly(ethylene glycol) nanoparticles enhance gene transfection.

The leading principle of non-viral delivery systems for gene therapy is to mediate high levels of gene expression with low cytotoxicity. Nowadays, biodegradable nanoparticles formulated with poly(lactic acid)-poly(ethylene glycol) (PLA-PEG) were wildly developed. However, the relative lower gene transfection efficiency and higher cytotoxicity still remained critical problems.

Mannan-modified solid lipid nanoparticles for targeted gene delivery to alveolar macrophages.

Purpose: Cationic solid lipid nanoparticles (SLN) have established themselves during the past decades. They can efficiently bind DNA directly via ionic interaction and mediate gene transfection. One major problem with SLN is the lack of cell-targeting ability.

Saccharide modified pharmaceutical nanocarriers for targeted drug and gene delivery.

Nanocarriers are effective non-viral vectors for drug and gene delivery with low immunogenicity in comparison with viral vectors. However, the lack of organ or cell specificity sometimes hinders their application and brings about unexpected side effects. Active targeting is an outstanding strategy recently developed for drug delivery systems, for example, surface modification of nanocarriers with specific ligands could target the pathological area to provide the maximum therapeutic efficacy.

Novel cationic SLN containing a synthesized single-tailed lipid as a modifier for gene delivery.

Cationic solid lipid nanoparticles (SLN) can bind DNA directly via ionic interaction and mediate in vitro gene transfection. However, toxicity is still an obstacle, which is strongly dependent on the cationic lipid used. In the present study, a novel single-tailed cationic lipid, 6-lauroxyhexyl lysinate (LHLN), was synthesized and used as a modifier to prepare stable SLN-DNA complexes by a nanoprecipitation method.

[Preparation of verapamil hydrochloride core-in-cup tablets with double-pulsatile and multi-phasic release].

To prepare verapamil hydrochloride (VH) core-in-cup tablets with tri-layered tablet and four-layered tablet as core tablets, separately, which can provide biphasic release with double-pulsatile and multi-phasic release, core tablets were prepared by direct compression method, and core-in-cup tablets by dry-compression coated technology. The parameter, time-lag (T(lag)), was used to evaluate the influence of factors, such as the weight of the top cover layer, the amount of hydroxypropylmethylcellulose (HPMC), and the compression load on VH release. With the increase of the weight and HPMC amount of the top cover layer, the first lag time T(lag1) was prolonged.