Expression dosage effects of a small number of genes after the artificial doubling of weeping forsythia.

Whole genome doubling (WGD) plays a critical role in plant evolution, yet the mechanisms underlying the maintenance of overall equilibrium following an artificial doubling event, as well as its impact on phenotype and adaptability, remain unclear. By comparing the gene expression of naturally occurring weeping forsythia diploids and colchicine-induced autotetraploids under normal growth conditions and cold stress, we identified gene expression dosage responses resulting from ploidy change. Only a small proportion of effectively expressed genes showed dosage effect, and most genes did not exhibit significant expression differences.

Comparative transcriptomics provides insights into the pathogenic immune response of brown leaf spots in weeping forsythia.

Weeping forsythia is an important ornamental, ecological and medicinal plant. Brown leaf spots limit the large-scale production of weeping forsythia as a medicinal crop. Alternaria alternata is a pathogen causing brown leaf spots in weeping forsythia; however, its pathogenesis and the immune response mechanisms of weeping forsythia remain unclear.

First Report of Stem Rot Caused by Botryosphaeria dothidea and Phomopsis velata on Forsythia suspensa in China.

Forsythia suspensa (Thunb.) Vahl (Oleaceae) is a well-known traditional Chinese medicine. It exhibits antioxidant activity and exerts antibacterial, antiviral, and antiemetic effects (Li and Chen 2005).

Comparative analysis of cold-responsive genes under short-term cold stimulation and cold-adaptive genes under long-term heterogeneous environments reveals a cold adaptation mechanism in weeping forsythia.

Identifying cold-related genes can provide insights into the cold adaptation mechanism of weeping forsythia. In this study, we compared the changes in gene expressions and physiological and biochemical indices under short-term cold stimulation with the changes in gene sequences under a long-term heterogeneous environment to investigate the cold adaptation mechanism in weeping forsythia. The data of adaptive gene sequence changes, e.

Characterization of the complete chloroplast genome of L. (Caprifoliaceae).

L. is an excellent landscaping shrub with high ornamental value. Here, we report the complete chloroplast genome sequence of .

The complete chloroplast genome sequence of L. (Cornaceae).

has been used as antiphlogistic, hemostatic and diuretic treatments in Korea, and which is one of the most important ornamental shrubs in China. Here, we reported the complete chloroplast genome sequence of . The size of the chloroplast genome is 158,451 bp in length, including a large single copy region (LSC) of 87,778 bp, a small single copy region (SSC) of 18,927 bp, and a pair of inverted repeated regions of 25,873 bp.

The complete chloroplast genome of Blume.

is a popular Chinese medicine, that has its place in the treatment of spasm, osteodynia of the lumbar region and knees. The complete chloroplast (cp) genome of was determined. The complete cp genome is 151,543 bp in length and includes a large single-copy (LSC) region of 83,922 bp, a small single-copy (SSC) region of 17,251 bp, and a pair of inverted repeat regions (IRs) of 25,185 bp.

The complete chloroplast genome of (L.) Santapau.

is a perennial plant in the Scrophulariaceae family that is traditionally used as sedative, astringent, febrifuge, and for skin eruptions. Here, we determined the complete chloroplast(cp) genome sequence for using genome skimming sequencing. The cp genome was 153,618 bp and consisted of a large single copy (LSC) region (84,834 bp), a small single copy (SSC) region (17,884 bp), and two inverted repeats (IRs) (25,450 bp).

Complete chloroplast genome sequence and phylogenetic analysis of .

is commonly known as treacle-mustard or wormseed wallflower with value for reducing high temperature and inducing diuresis. Here, we characterized the complete chloroplast (cp) genome of using genome skimming sequencing. The circular complete cp genome is 154,611 bp in length, containing a large single-copy (LSC) region of 83,809 bp, two copies of IR (26,475 bp each) regions, and a small single-copy (SSC) region of 17,852 bp.

Butenolide Derivatives with α-Glucosidase Inhibitions from the Deep-Sea-Derived Fungus YPGA10.

Three new butenolide derivatives, namely aspernolides N-P (-), together with six known analogues (-), were isolated from the ethyl acetate (EtOAc) extract of the deep sea-derived fungus YPGA10. The structures of compounds - were determined on the basis of comprehensive analyses of the nuclear magnetic resonance (NMR) and mass spectroscopy (MS) data, and the absolute configurations of and were determined by comparisons of experimental electronic circular dichroism (ECD) with calculated ECD spectra. Compound represents the rare example of -derived butenolide derivatives featured by a monosubstituted benzene ring.

Two new meroterpenoids and two new monoterpenoids from the deep sea-derived fungus Penicillium sp. YPGA11.

Twelve terpenoids, including two new 3,5-dimethylorsellinic acid-based meroterpenoids (1 and 2) and two new monoterpenoids (11 and 12), were obtained from the deep-sea fungus Penicillium sp. YPGA11. Their structures were determined by extensive analyses of spectroscopic data, and the absolute configurations of 1 and 2 were determined by comparisons of experimental and calculated ECD spectra.

Peniginsengins B⁻E, New Farnesylcyclohexenones from the Deep Sea-Derived Fungus sp. YPGA11.

Chemical examination of the EtOAc extract of the deep sea-derived fungus sp YPGA11 resulted in the isolation of four new farnesylcyclohexenones, peniginsengins B⁻E (⁻), and a known analog peniginsengin A (). The structures of compounds ⁻ were determined on the basis of comprehensive analyses of the nuclear magnetic resonance (NMR) and mass spectroscopy (MS) data, and the absolute configurations of , , and were determined by comparisons of experimental electronic circular dichroism (ECD) with calculated ECD spectra. Compounds ⁻, characterized by a highly oxygenated 1-methylcyclohexene unit and a (4,8)-4,8-dimethyldeca-4,8-dienoic acid side chain, are rarely found in nature.

Penicindopene A, a new indole diterpene from the deep-sea fungus sp. YPCMAC1.

A new indole diterpene, named penicindopene A (), together with seven known compounds (-), was isolated from the deep-sea fungus sp. YPCMAC1. The structure of penicindopene A was elucidated by extensive spectroscopic analyses (1 D and 2 D NMR, and HRESIMS data), in addition to the ECD calculations for the assignments of its absolute configuration.

The First Genetic Map in Sweet Osmanthus ( Lour.) Using Specific Locus Amplified Fragment Sequencing.

is an ornamental plant of substantial commercial value, and no genetic linkage maps of this species have previously been reported. Specific-locus amplified fragment sequencing (SLAF-seq) is a recently developed technology that allows massive single nucleotide polymorphisms (SNPs) to be identified and high-resolution genotyping. In our current research, we generated the first genetic map of using SLAF-seq, which is composed with 206.

Characterization of OfWRKY3, a transcription factor that positively regulates the carotenoid cleavage dioxygenase gene OfCCD4 in Osmanthus fragrans.

The sweet osmanthus carotenoid cleavage dioxygenase 4 (OfCCD4) cleaves carotenoids such as β-carotene and zeaxanthin to yield β-ionone. OfCCD4 is a member of the CCD gene family, and its promoter contains a W-box palindrome with two reversely oriented TGAC repeats, which are the proposed binding sites of WRKY transcription factors. We isolated three WRKY cDNAs from the petal of Osmanthus fragrans.

[Identify the original plant of Forsythia suspensa by nucleotide sequence].

Objective: To investigate the means of distinguishing the original plant of Forsythia suspensa from confusion.

Methods: To amplify the chloroplast matK gene by PCR using a consensus primer set and determine their nucleotide sequence by PCR direct sequencing. The ITS sequences were gained from NCBI.