Delegated Proof of Stake Consensus Mechanism Based on Community Discovery and Credit Incentive.

Consensus algorithms are the core technology of a blockchain and directly affect the implementation and application of blockchain systems. Delegated proof of stake (DPoS) significantly reduces the time required for transaction verification by selecting representative nodes to generate blocks, and it has become a mainstream consensus algorithm. However, existing DPoS algorithms have issues such as "one ballot, one vote", a low degree of decentralization, and nodes performing malicious actions.

Role of circadian clock in the chronoefficacy and chronotoxicity of clopidogrel.

Background And Purpose: The role of circadian locomotor output cycles kaput (CLOCK) in regulating drug chronoefficacy and chronotoxicity remains elusive. Here, we aimed to uncover the impact of CLOCK and dosing time on clopidogrel efficacy and toxicity.

Experimental Approach: The antiplatelet effect, toxicity and pharmacokinetics experiments were conducted with Clock mice and wild-type mice, after gavage administration of clopidogrel at different circadian time points.

Epigenetic Application of ATAC-Seq Based on Tn5 Transposase Purification Technology.

Background: Assays of transposase accessible chromatin sequencing (ATAC-seq) is an efficient assay to investigate chromatin accessibility, which depends on the activity of a robust Tn5 transposase to fragment the genome while cutting in the sequencing adapters.

Methods: We propose reliable approaches for purifying hyperactive Tn5 transposase by chitin magnetic bead sorting. Double-stranded DNA of J76 cells and 293T cells were digested and subjected to tagmentation as test samples with Tn5 transposase, and libraries were established and sequenced.