[Regulation of tissue factor expression in brain microvascular endothelial cells by PLA nanoparticles coating NF-kappaB decoy oligonucleotides].

Objective: To investigate a new strategy of polylactic acid (PLA) nanoparticles delivery system coating nuclear factor-kappaB (NF-kappaB) decoy oligonucleotides (ODNs) for inhibiting TF expression in cultured brain microvascular endothelial cells(BMECs).

Methods: PLA nanoparticles coating FITC-labeled NF-kappaB decoy ODNs were formulated by nano-deposition method and the characteristics of nanoparticles were detected. BMECs were isolated and cultured in vitro.

[Polylactic acid nanoparticles across the brain-blood barrier observed with analytical electron microscopy].

The blood-brain barrier (BBB) is a huge obstacle in therapy of brain diseases, for it hinders the delivery of water-soluble molecules and those with molecular weight above 500 from the circulation system to the brain. Polysorbate 80 (Tween 80, T-80)-coated polylactid acid(PLA) nanoparticles represent a tool to transport such drugs across the BBB. Transcytosis is put forward as one mechanism of drug-loaded nanoparticles across the blood-brain barrier (BBB).