is one of the most important traditional Chinese Orchids due to its unique and highly ornamental floral organs. Although the ABCDE model for flower development is well-established in model plant species, the precise roles of these genes in are not yet fully understood. In this study, four -like genes were isolated and identified from .
View Article and Find Full Text PDFAims And Objectives: To examine the effectiveness of a nurse-led transitional care programme on readmission, self-efficacy to implement health-promoting behaviours, functional status and life quality among Chinese patients with coronary artery disease.
Background: Coronary artery disease is a major cause of mortality in China. Transitional care could help to ensure improved patient outcomes.
Studies on the occurrence of homebound and the factors influencing it are available. However, the study of community homebound in China is still in its preliminary stage. No previous studies about this issue are available.
View Article and Find Full Text PDFBackground: Coronary artery disease is a major cause of morbidity and mortality among adults worldwide, including China. After a hospital stay, transitional care could help to ensure improved patient care and outcomes, and reduce Medicare costs. Nevertheless, the results of the existing transitional care are not always satisfactory and our knowledge of how to perform effective transitional care for patients with coronary artery disease is limited in mainland China.
View Article and Find Full Text PDFThe ability of lymphocytes and macrophage-derived cytokines and chemokines to modulate the activation of stromal cells during immune responses is well-documented, but few studies have investigated whether liver myofibroblasts shape the phenotype and function of monocytes in liver disease. In the present study, Kupffer cells were demonstrated to be activated in the inflamed livers of patients with cirrhosis and be in close contact with liver myofibroblasts. The Kupffer cells from cirrhotic livers expressed significantly elevated levels of PD-L1 (also termed B7-H1), TLR4, CD80, CD32 and CD64 relative to those from normal livers.
View Article and Find Full Text PDFZhongguo Dang Dai Er Ke Za Zhi
June 2009
Objective: Human telomerase reverse transcriptase (hTERT) is a rate-limiting enzyme which dictates the activity of human telomerase and thus decides the life span of cells. The aim of this study was to explore the expression of hTERT in bone marrow from children with beta-thalassemia major and the relationship between the expression of hTERT and hemoglobin levels.
Methods: Multiple allele specific polymerase chain reaction (MASPCR) was used for targeted DNA amplification and gene mutation analysis of beta-thalassemia.
Sichuan Da Xue Xue Bao Yi Xue Ban
May 2004
Objective: This genetic analysis on 27 children with beta-thalassemia major and their parents was performed in an attempt to elucidate the characteristics of gene mutations, and to improve the early diagnosis and prevention of this disease in Sichuan area.
Methods: The hematologic studies of beta-thalassemia included the osmotic fragility of erythrocyte determined by brine osmosis method, the HbF qualitatively determined by one minute anti-alkaline method, the HbA2 assayed by acetyl-cellulose membrane electrophoresis and eluate photometric method, and the total hemoglobin measured by the ferric-cynade method. DNA was extracted from peripheral white blood cell with standard method (phenol-chloroform extract).
Zhonghua Yi Xue Yi Chuan Xue Za Zhi
February 2003
Objective: To explore the relationship between gene expression of human telomerase reverse transcriptase (hTERT) and its clinical characteristics in leukemia.
Methods: The protocol of RT-PCR was used to detect the hTERTmRNA expressing levels in peripheral blood samples from leukemic patients under primary treatment(n=42), in complete remission(n=21), with recurrent leukemia (n=4); and from normal subjects (n=5), respectively.
Results: The positive percentage of hTERTmRNA expression was 73.
The genetic diversity and differentiation of Lilium formolongi Hort. lines in self-inbred early generations were studied by allozyme electrophoresis. The results showed that mean allele number/locus, percentage of polymorphic loci, average observed heterozygosity and average expected heterozygosity were decreased with the increment of self-inbred generations.
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