Analysis of doxorubicin uptake in single human leukemia K562 cells using capillary electrophoresis coupled with laser-induced fluorescence detection.

The doxorubicin (DOX) uptake in single human leukemia K562 cells with changes in both drug dosage and exposure period was studied using capillary electrophoresis (CE) coupled with laser-induced fluorescence (LIF) detection. The cells were treated with DOX at different concentrations (1, 3, 10, 20, 30, and 50 μM) and for different exposure times (1, 3, 5, 24, and 48 h). At least 20 cells were analyzed for each DOX-treated cell population.

Magnifying endoscopy for monitoring intestinal adaptation: a case report.

A 33-year-old Chinese man with short bowel syndrome received intestinal rehabilitation therapy. We observed microscopically morphologic changes of remnant small intestine by magnifying endoscopy and histopathologic examination before and after 3 courses of treatment. The results revealed that the 2 methods have a fairly good correlativity, and magnifying endoscopy examination of intestinal adaptation in patients with short bowel syndrome possesses great applicative value.

Reversible transition between thermodynamically stable phases with low density of oxygen vacancies on the SrTiO3(110) surface.

The surface reconstruction of SrTiO3(110) is studied with scanning tunneling microscopy and density functional theory (DFT) calculations. The reversible phase transition between (4×1) and (5×1) is controlled by adjusting the surface metal concentration [Sr] or [Ti]. Resolving the atomic structures of the surface, DFT calculations verify that the phase stability changes upon the chemical potential of Sr or Ti.

[Adjuvant chemotherapy for gastric cancer: more drugs do not mean better efficacy].

Objective: To compare oncologic outcomes between doublet and triplet adjuvant chemotherapy for gastric cancer patients undergoing radical resection.

Methods: Patients with gastric cancer receiving adjuvant chemotherapy after radical resection from January 2004 to December 2008 were included. Doublet was defined as 5-FU 750 mg/m² (days 1-5) or capecitabine 1000 mg/m² (days 1-14) plus cisplatin 60 mg/m² (day 1) or oxaliplatin 130 mg/m² (day 1), while triplets had epirubicin 50 mg/m² (day 1) added.

Origins of 1/f noise in nanostructure inclusion polymorphous silicon films.

In this article, we report that the origins of 1/f noise in pm-Si:H film resistors are inhomogeneity and defective structure. The results obtained are consistent with Hooge's formula, where the noise parameter, αH, is independent of doping ratio. The 1/f noise power spectral density and noise parameter αH are proportional to the squared value of temperature coefficient of resistance (TCR).

Full emission color tuning in luminogens constructed from tetraphenylethene, benzo-2,1,3-thiadiazole and thiophene building blocks.

Full color luminogens are constructed from tetraphenylethene, benzo-2,1,3-thiadiazole and thiophene building blocks. OLED fabricated using one of the luminogens exhibits orange-red electroluminescence with high luminance and efficiencies of 8330 cd m(-2), 6.1 cd A(-1) and 3.

MAGI1 inhibits cancer cell migration and invasion of hepatocellular carcinoma via regulating PTEN.

Objective: To explore the biological function and molecular mechanism of membrane associated guanylate kinase, WW and PDZ domain containing 1 (MAGI1) in hepatocellular carcinoma.

Methods: HepG2(MAGI1) stable cell line was constructed by transfecting HepG2 cells with pcDNA3.1-MAGI1 plasmid.

Nanoscale footprints of self-running gallium droplets on GaAs surface.

In this work, the nanoscale footprints of self-driven liquid gallium droplet movement on a GaAs (001) surface will be presented and analyzed. The nanoscale footprints of a primary droplet trail and ordered secondary droplets along primary droplet trails are observed on the GaAs surface. A well ordered nanoterrace from the trail is left behind by a running droplet.

Characteristics of particulate matter pollution in the Pearl River Delta region, China: an observational-based analysis of two monitoring sites.

Two-year monitoring data (2006 and 2009), collected at the sub-urban site (WQS) and the background site (TH), were used to study the characteristics of Particulate Matter (PM) pollution in the Pearl River Delta region, China. Similar levels of PM(2.5) concentration measured at both sites seem to confirm that the fine particles have emerged as a major regional pollution issue.

On the secondary droplets of self-running gallium droplets on GaAs surface.

Self-running droplets by thermal evaporation GaAs (001) surface are studied and analyzed using a scanning electron microscope. The sample is prepared under high-temperature annealing in an ultrahigh vacuum molecular beam epitaxy system. Particularly, secondary droplets which formed along primary droplet running trails are investigated.

Whole-genome sequences of four Mycobacterium bovis BCG vaccine strains.

Mycobacterium bovis Bacille Calmette-Guérin (BCG) is the only vaccine available against tuberculosis (TB). A number of BCG strains are in use, and they exhibit biochemical and genetic differences. We report the genome sequences of four BCG strains representing different lineages, which will help to design more effective TB vaccines.

Near infrared broadband emission of In0.35Ga0.65As quantum dots on high index GaAs surfaces.

The morphology and optical properties of In(0.35)Ga(0.65)As/GaAs quantum dots (QDs) grown on (210), (311)A, (711)A, (731) and (100) substrates are investigated.

Diversity through phosphine catalysis identifies octahydro-1,6-naphthyridin-4-ones as activators of endothelium-driven immunity.

The endothelium plays a critical role in promoting inflammation in cardiovascular disease and other chronic inflammatory conditions, and many small-molecule screens have sought to identify agents that prevent endothelial cell activation. Conversely, an augmented immune response can be protective against microbial pathogens and in cancer immunotherapy. Yet, small-molecule screens to identify agents that induce endothelial cell activation have not been reported.

Aplexone targets the HMG-CoA reductase pathway and differentially regulates arteriovenous angiogenesis.

Arterial and venous endothelial cells exhibit distinct molecular characteristics at early developmental stages. These lineage-specific molecular programs are instructive to the development of distinct vascular architectures and physiological conditions of arteries and veins, but their roles in angiogenesis remain unexplored. Here, we show that the caudal vein plexus in zebrafish forms by endothelial cell sprouting, migration and anastomosis, providing a venous-specific angiogenesis model.

Lentiviral-mediated siRNA targeted against osteopontin suppresses the growth and metastasis of gastric cancer cells.

The expression of osteopontin (OPN) has been correlated with tumor growth and metastasis. However, the mechanisms by which OPN promotes tumor metastasis remain unclear. In this study, we aimed to investigate the anti-tumor effects of OPN by silencing OPN expression in the gastric cancer cell line SGC7901, using lentiviral-OPN small interfering RNA (siRNA) technology.

Diversity through a branched reaction pathway: generation of multicyclic scaffolds and identification of antimigratory agents.

A library of 91 heterocyclic compounds composed of 16 distinct scaffolds has been synthesized through a sequence of phosphine-catalyzed ring-forming reactions, Tebbe reactions, Diels-Alder reactions, and, in some cases, hydrolysis. This effort in diversity-oriented synthesis produced a collection of compounds that exhibited high levels of structural variation both in terms of stereochemistry and the range of scaffolds represented. A simple but powerful sequence of reactions thus led to a high-diversity library of relatively modest size with which to explore biologically relevant regions of chemical space.

Lung cancer mortality from exposure to chrysotile asbestos and smoking: a case-control study within a cohort in China.

Objective: To confirm the association between exposure to chrysotile asbestos and lung cancer risk and to demonstrate the combined effect of smoking and asbestos exposure.

Methods: A case-control study of 1139 asbestos workers identified 41 male lung cancer cases in 2001; each case was matched by age (±5 years) with five controls. Workers in seven workshops were categorised into high-, medium- and low-exposure subgroups, and conditional logistic regression was applied to estimate the odds ratios for lung cancer risk associated with the different exposure levels.

[RNA interference of HDAC7 expression in hepatocellular carcinoma].

Objective: To investigate the role of histone deacetylase 7 (HDAC7) in the occurrence and development of hepatocellular carcinoma.

Methods: HepG2 cells and human microvascular endothelial cells (HMEC-1) were divided into 3 groups after transfection pSUPER-HDAC7 retroviral interference plasmid: a pSUPER(HDAC7RNAi+) group, a pSUPER(HDAC7RNAi-) group, and a pSUPER group as the control group. The expression of HDAC7, p21, cyclin E, matrix metalloproteinases 10 (MMP10), and hypoxiainducible factor-1alpha (HIF-1alpha) were detected by Western blot.

Superlattice Growth via MBE and Green's Function Techniques.

A model has been developed to simulate the growth of arrays consisting of a substrate on which alternating layers of quantum dots (QDs) and spacer layers are epitaxially grown. The substrate and spacer layers are modeled as an anisotropic elastic half-space, and the QDs are modeled as point inclusions buried within the half-space. In this model, the strain at the free surface of this half-space due to the buried point QDs is calculated, and a scalar measure of the strain at the surface is subsequently determined.

Multilayers of InGaAs Nanostructures Grown on GaAs(210) Substrates.

Multilayers of InGaAs nanostructures are grown on GaAs(210) by molecular beam epitaxy. With reducing the thickness of GaAs interlayer spacer, a transition from InGaAs quantum dashes to arrow-like nanostructures is observed by atomic force microscopy. Photoluminescence measurements reveal all the samples of different spacers with good optical properties.

InGaAs Quantum Well Grown on High-Index Surfaces for Superluminescent Diode Applications.

The morphological and optical properties of In0.2Ga0.8As/GaAs quantum wells grown on various substrates are investigated for possible application to superluminescent diodes.

Prognostic significance of neuroepithelial transforming protein 1 in hepatocellular carcinoma.

Objective: Recent works have demonstrated that neuroepithelial transforming protein 1 (NET-1) gene expression is associated with proliferation, metastasis, and clinical stages of human hepatocellular carcinoma (HCC). To investigate its prognostic significance in HCC, which currently is unknown, the authors examined the correlation between NET-1 expression and prognosis in patients with HCC.

Methods: Immunohistochemical staining was used to determine NET-1 expression level in 368 paired HCC and normal liver tissue (NLT) specimens.

[Effect of occupational stressors on coping resources of nurses in Nanchang].

Objective: To study the effects of occupational stressors on coping resources of nurses.

Methods: Coping resources (recreation, self-care, social support, rational coping) and occupational stressors (role overload, role insufficiency, role ambiguity, role boundary, responsibility, physical environment) were measured on 387 nurses by personal resources questionnaire (PRQ) and Occupational Role Questionnaire (ORQ) respectively.

Results: The higher the level of occupational stressors was, the lower the coping resources of nurses were (P < 0.

Non-recurrent laryngeal nerve related to thyroid surgery: a report of 5 cases and literature review.

Background: Non-recurrent laryngeal nerve, a rare anomaly, passes transversely into larynx with or without its recurrent branches directly arising from the vagus nerve. The paper investigates clinical significance of non-recurrent laryngeal nerve during thyroid surgery. Clinical data from 5 cases of non-recurrent laryngeal nerve and related literature reviews were made to acknowledge its incidence, variant types and matters concerned during thyroid operations.