A lipid droplet-targeted probe for imaging of lipid metabolism disorders during mitochondrial myopathy.

Lipid metabolism is closely related to various biological processes in cells. The accumulation of Lipid droplets (LDs) is a typical manifestation of certain metabolic diseases, such as mitochondrial myopathy, which shows a significant increase in LDs. The accumulation of LDs can exacerbate the progression of disease, and lysosomes selectively degrade LDs to cope with this phenomenon.

Begomovirus Transmission by the Insect Vector, the Whitefly Bemisia tabaci.

Begomoviruses are exclusively transmitted by the whitefly Bemisia tabaci in a persistent circulative manner and pose serious threat to the production of important cash crops worldwide. Here, we describe the protocols to study how the whiteflies transmit begomoviruses, including maintenance of whitefly and species identification, preparation of tomato plants infected with virus, acquisition and transmission of virus by whiteflies, dissection of whitefly organs, analysis of virus quantity in whiteflies, immunofluorescence and fluorescence in situ hybridization (FISH) detection of virus in whitefly tissues, membrane feeding of double-stranded RNA (dsRNA) or antibody and microinjection of dsRNA in whiteflies.

Prognostic and predictive values of a multimodal nomogram incorporating tumor and peritumor morphology with immune status in resectable lung adenocarcinoma.

Background: Current prognostic and predictive biomarkers for lung adenocarcinoma (LUAD) predominantly rely on unimodal approaches, limiting their characterization ability. There is an urgent need for a comprehensive and accurate biomarker to guide individualized adjuvant therapy decisions.

Methods: In this retrospective study, data from patients with resectable LUAD (stage I-III) were collected from two hospitals and a publicly available dataset, forming a training dataset (n=223), a validation dataset (n=95), a testing dataset (n=449), and the non-small cell lung cancer (NSCLC) Radiogenomics dataset (n=59).

Self-Referenced Probe Enables Quantified Monitoring Mitochondrial Membrane Potential by Colocalization Coefficients.

Mitochondria are dynamic organelles that have a variety of physiological states. Mitochondrial membrane potential (MMP) maintains mitochondrial physiological activities and reflects cellular health and viability. Thus, it is essential to dynamically monitor changes in MMP for understanding mitochondria-related biological processes.

Comparison of the Immune Enhancing Activity and Chemical Constituents Between Imitation Wild and Cultivated Astragali Radix.

Astragali Radix (AR), a traditional food and medicinal herb used for thousands of years, is widely recognized for its role in enhancing immunity, particularly when combined with adjuvant chemotherapy. The two primary types of AR available in the market are imitation wild AR (grown for seven years) and cultivated AR (grown for two years). However, whether differences exist in their immune-enhancing effects and chemical constituents remains unclear.

Regulating Precursor Viscosity with Inert Solvent Additives for Efficient Blade-Coated Perovskite Solar Cells.

Metal halide perovskite solar cells (PSCs) are emerging as promising candidates for next-generation photovoltaics aimed at green energy production. However, during solution-processed film deposition, the distinct rheological behaviors of blade coating, compared to spin coating, result in less controlled crystallization, leading to inferior film quality and limiting the power conversion efficiency (PCE) of blade-coated photovoltaics. In this work, ethylene glycol (EG) is introduced as an inert co-solvent in perovskite precursor solutions to achieve high-quality perovskite films via blade coating.

Discovery of a p-Type Thermoelectric Material in the [Bi][BiQ] Family through the Regulation of Carrier Concentration.

In recent years, many potential n-type near-room-temperature thermoelectric materials belonging to the [Bi][BiQ] family have been discovered, whereas new p-type thermoelectric materials are relatively rare. In this study, Sb is doped based on n-type BiSbTe by adjusting the carrier concentration and then transforms the materials into p-type thermoelectric materials. A novel p-type near-room-temperature thermoelectric material, BiSbTe, is discovered in this family.

A novel strategy for using ficin enzyme from fig leaves to extract collagen from tannery-trimming wastes.

There is a growing interest in the utilization of animal by-products and wastes. Plant-derived enzymes could play a vital role in collagen extraction from tannery-trimming wastes. Here, the ficin enzyme from fig leaves was used for collagen extraction and compared with conventional acetic acid and pepsin extraction methods, aiming to assess the efficiency and purity of collagen extracted using this plant-based enzyme approach relative to the organic solvents and animal-derived enzymes.

Multimodal radiopathological integration for prognosis and prediction of adjuvant chemotherapy benefit in resectable lung adenocarcinoma: A multicentre study.

Lung adenocarcinoma (LUAD) has a heterogeneous prognosis and controversial postoperative treatment protocols. We aim to develop and validate a multimodal analysis framework that integrates CT images with H&E-stained whole-slide images (WSIs) to enhance risk stratification and predict adjuvant chemotherapy benefit in LUAD patients. We retrospectively collected data from 1039 resectable LUAD patients (stage I-III) across four centres, forming a training dataset (n = 303), two testing datasets (n = 197 and n = 228) for survival analysis, and a feature testing dataset (n = 311) for interpretability analysis.

Study on the Chemical Constituents of Different Parts of Xanthoceras sorbifolia Bunge Based on Plant Metabolomics.

Xanthoceras sorbifolia is valued in food, landscape, and medicine, with its husk containing triterpenoid saponin known for anti-Alzheimer's effects. This study, using ultra-high-performance liquid chromatography coupled with high-resolution mass spectrometry and plant metabolomics, compared the chemical profiles of different parts of X. sorbifolia to assess their potential for replacing the husk in medicinal use.

Folic acid alleviates the negative effects of dexamethasone induced stress on production performance in Hyline Brown laying hens.

Multiple stressors are believed to deteriorate production performance and cause substantial economic losses in commercial poultry farming. Folic acid (FA) is an antioxidant compound that can improve oocyte function and regulate gut microbiota composition. The current study was conducted to investigate the role of FA in alleviating stress and improving production performance.

SUMOylation regulates GSDMD stability and pyroptosis.

Various post-translational modifications (PTMs), such as palmitoylation, acetylation, and ubiquitination, have been shown to regulate pyroptosis. However, the role of small ubiquitin-like modifier (SUMO) modification, known as SUMOylation, in regulating GSDMD activity and pyroptosis remains unclear. Here, we demonstrate that inhibition of SUMOylation reduces inflammatory pyroptosis by downregulating GSDMD expression.

Identification of deep intronic variants in junctional epidermolysis bullosa using genome sequencing and splicing assays.

Junctional epidermolysis bullosa (JEB) is characterized by mucocutaneous fragility. We enrolled 69 cases of recessive JEB, with 13.0% of these cases remained genetically undiagnosed following an initial exome sequencing.

The variant c.1670G>A in the SREBF1 gene is associated with unusual clinical manifestations of IFAP syndrome.

Ichthyosis follicularis, atrichia and photophobia (IFAP) syndrome is a rare genetic genodermatosis. According to previous reports, in addition to MBTPS2 variants, variants in SREBF1 (encoding SREBP1) can also cause IFAP syndrome. SREBF1 variants can also result in hereditary mucoepithelial dysplasia (HMD).

Succinate supplementation alleviates liver cancer by inhibiting the FN1/SQLE axis-mediated cholesterol biosynthesis.

Succinate is a crucial metabolite in the TCA cycle and contributes to cancer development. However, the role of exogenous succinate in hepatocellular carcinoma (HCC) is unclear. Here, we report that the concentration of succinate in HCC tissues is lower compared to adjacent normal tissues, as determined by spatial metabolomics and quantitative metabolomics analysis.

Tailored Colloidal Shapes in Precursor Solutions for Efficient Blade-Coated Perovskite Solar Modules.

Metal halide perovskite solar cells (PSCs) have emerged as one of the most promising candidates for next-generation photovoltaic technologies. However, perovskite films deposited by blade-coating usually exhibit inferior film morphology compared to those fabricated by spin-coating, which hinders the power conversion efficiency (PCE) and stability of the scalable perovskite solar modules (PSMs). Herein, ellipsoidal colloids are tailored in the perovskite precursor solution by incorporating perovskite colloids and polymer additives.

Effects of basic type of intermittent exotropia on myopic shift in children: a 12-month observational study.

Background: To investigate the effect of basic intermittent exotropia (IXT) on myopic shift in children during 12-month follow-up.

Methods: 165 children aged 4-15 years were recruited prospectively in this study and divided into 3 groups: Group A, consisted of 64 patients with basic IXT without surgery; Group B, consisted of 51 patients 1-month after IXT-corrected surgery; and Group C, consisted of 50 patients without any form of strabismus. All patients underwent assessments of spherical equivalent (SE), axial length (AL), exodeviation, and binocular function relating to accommodation and convergence.

Fecal microbiota transplantation combined with inulin promotes the development and function of early immune organs in chicks.

Modern management of chicks hinders the vertical transmission of intestinal microbiota, which is closely related to immunity. Inulin is a substrate that can be utilized by the microbiota. This study aimed to determine whether fecal microbiota transplantation (FMT) combined with inulin played a "1 + 1 > 2" role in enhancing the development and function of immune organs.

Scutellarin inhibits pyroptosis via selective autophagy degradation of p30/GSDMD and suppression of ASC oligomerization.

Most of the pyroptosis inhibitors targeted Gasdermin D (GSDMD) are functioning by restraining GSDMD-N (p30) oligomerization. For the first time, this work discovered a pyroptosis inhibitor taking effect by degrading p30 and GSDMD. As the principal bioactive constituent in Erigeron breviscapus, scutellarin (SCU) assumes a pivotal role in the realm of anti-inflammatory processes.

Pathways to usage intention of mobile health apps among hypertensive patients: A fuzzy-set qualitative comparative analysis.

The efficacy of mHealth apps in managing hypertension has been proven; however, low usage intention remains a significant challenge, warranting an in-depth exploration of the influencing factors. This study aimed to examine the factors influencing hypertensive new users' intention to use mobile health applications through a cross-sectional survey. Fuzzy-set qualitative comparative analysis (fsQCA) was employed to investigate the combinations of various determinants, including technology acceptance, adoption factors, compliance behavior initiation factors, and time motivation factors for decision making.

Genome-Wide Characterization and Analysis of the Gene Family in .

(L.) Britt. is a traditional medicinal and culinary plant with a long history of cultivation and significant potential for broader utilization.

Liquid chromatography coupled with high resolution mass spectrometry reveals the inhibitory effects of Huangkuisiwu formula on biosynthesis of protein-binding uremic toxins in rats with chronic kidney disease.

Chronic kidney disease (CKD) is recognized as a common disorder worldwide. Protein-binding uremic toxins that cannot be efficiently removed by extracorporeal renal replacement therapies, such as indoxyl sulfate (IS) and p-cresyl sulfate (PCS), are associated with high risks of cardiovascular complications and high mortality in CKD population. This study aimed to explore the therapeutical effects of Huangkuisiwu formula (HKSWF) on CKD rats.

Delactylation diminished the growth inhibitory role of CA3 by restoring DUOX2 expression in hepatocellular carcinoma.

Lactylation is an emerging pathogenesis of hepatocellular carcinoma (HCC). However, the underlying mechanisms and biological significance remain poorly understood. The Carbonic anhydrase III (CA3) gene, previously defined as a binding protein of SQLE and involved in the NAFLD disease, has now been identified as a novel tumor suppressor in HCC.

Accuracy of virtual reality in automated measurement and diagnosis of strabismus.

Background: To explore the accuracy of VR technology for strabismus screening.

Methods: In this cross-sectional study, a total of 191 subjects were included. Examinations included VR technology based on infrared, the cover-uncover test, and the prism alternating cover test (PACT) at various gaze positions, specifically at distances of 33 cm and 6 m.