[Research of the EEMD method to pulse analysis of traditional Chinese medicine based on different amplitudes of the added white noise].

The ensemble empirical mode decomposition (EEMD) can be used to overcome the mode mixing problem of empirical mode decomposition (EMD) effectively. The EEMD method and Hilbert-Huang Transform (HHT) can be used to analyze pulse signals of Traditional Chinese Medicine (TCM). The amplitudes of the added white noise were about 0.

[Automatic classification of lip color based on SVM in traditional Chinese medicine inspection].

The lip color of a person is closely related to his or her health in the visual diagnosis of traditional Chinese medicine (TCM). The traditional method to judge the color of lips is through observing by a TCM doctor. The diagnosis result is affected not only by the doctor's knowledge and diagnosis experience, but also by the light, temperature and other environmental impacts.

Semi-dominant mutations in the CC-NB-LRR-type R gene, NLS1, lead to constitutive activation of defense responses in rice.

In this study, we characterized the semi-dominant mutant nls1-1D (necrotic leaf sheath 1) of rice, which displays spontaneous lesions, specifically on leaf sheaths, with a developmental pattern. nls1-1D plants also exhibited constitutively activated defense responses, including extensive cell death, excess hydrogen peroxide and salicylic acid (SA) accumulation, up-regulated expressions of pathogenesis-related genes, and enhanced resistance to bacterial pathogens. Map-based cloning revealed that NLS1 encodes a typical CC-NB-LRR-type protein in rice.

Drosophila morgue influences cell numbers and positions in the embryonic nervous system.

Morgue is a unique multi-domain protein that contains a zinc finger motif, an F box, and a variant E2 conjugase domain. The presence of these domains suggests potentially complex and novel functions for Morgue in ubiquitination pathways. Morgue was originally identified via its gain-of-function enhancement of eye cell death phenotypes in Drosophila and ectopic expression of Morgue also influences circadian rhythms.

Objective research of auscultation signals in Traditional Chinese Medicine based on wavelet packet energy and support vector machine.

This study aims at utilising Wavelet Packet Transform (WPT) and Support Vector Machine (SVM) algorithm to make objective analysis and quantitative research for the auscultation in Traditional Chinese Medicine (TCM) diagnosis. First, Wavelet Packet Decomposition (WPD) at level 6 was employed to split more elaborate frequency bands of the auscultation signals. Then statistic analysis was made based on the extracted Wavelet Packet Energy (WPE) features from WPD coefficients.

Nitric oxide: promoter or suppressor of programmed cell death?

Nitric oxide (NO) is a short-lived gaseous free radical that predominantly functions as a messenger and effector molecule. It affects a variety of physiological processes, including programmed cell death (PCD) through cyclic guanosine monophosphate (cGMP)-dependent and - independent pathways. In this field, dominant discoveries are the diverse apoptosis networks in mammalian cells, which involve signals primarily via death receptors (extrinsic pathway) or the mitochondria (intrinsic pathway) that recruit caspases as effector molecules.

ZEBRA2, encoding a carotenoid isomerase, is involved in photoprotection in rice.

"zebra" mutants have alternating green and chlorotic crossbands on leaf blades and are widely distributed in monocotyledonous crops. Most recently, we cloned the first responsible gene from rice, ZEBRA2, which also leads to the phenotype of rice preharvest sprouting. ZEBRA2, a single-copy gene in the rice genome, encodes a carotenoid isomerase (CRTISO), the key enzyme catalyzing the conversion of cis-lycopene to all-trans lycopene.

Application of voice signal collection and analysis in traditional Chinese medicine syndrome differentiation of deficiency and excess.

Objective: To provide more objective basis for traditional Chinese medicine (TCM) syndrome differentiation of deficiency and excess by collecting and analyzing voice signals and extracting characteristic parameters.

Methods: All of 308 samples including 150 samples of qi-deficiency, 52 yin-deficiency, 55 excess and 51 normal were collected by "Voice Collecting System of TCM" and analyzed by wavelet packet transform (WPT) combined with approximate entropy (ApEn). The characteristic parameters with remarkable differences were chosen as input vectors for support vector machine (SVM) classifier to obtain classification results.

Conditioned medium from renal tubular epithelial cells stimulated by hypoxia influences rat bone marrow-derived endothelial progenitor cells.

Background And Aims: It has been well documented that endothelial progenitor cells (EPCs) participate in neovascularization in adults and that rarefaction of peritubular capillaries (PTCs) is closely associated with progressive kidney disease. Therefore, we investigated whether EPCs were influenced by conditioned medium (CM) of renal tubular epithelial cells (RTECs) stimulated by hypoxia, to provide evidence for EPCs transplantation in vivo in the future.

Methods: Mononuclear cells of rat bone marrow were isolated by density gradient centrifugation and were cultured according to previously described techniques.

Modelling of inquiry diagnosis for coronary heart disease in Traditional Chinese Medicine by using multi-label learning.

Background: Coronary heart disease (CHD) is a common cardiovascular disease that is extremely harmful to humans. In Traditional Chinese Medicine (TCM), the diagnosis and treatment of CHD have a long history and ample experience. However, the non-standard inquiry information influences the diagnosis and treatment in TCM to a certain extent.

Coordinated regulation of active and repressive histone methylations by a dual-specificity histone demethylase ceKDM7A from Caenorhabditis elegans.

H3K9me2 and H3K27me2 are important epigenetic marks associated with transcription repression, while H3K4me3 is associated with transcription activation. It has been shown that active and repressive histone methylations distribute in a mutually exclusive manner, but the underlying mechanism was poorly understood. Here we identified ceKDM7A, a PHD (plant homeodomain)- and JmjC domain-containing protein, as a histone demethylase specific for H3K9me2 and H3K27me2.

Structural insights into a dual-specificity histone demethylase ceKDM7A from Caenorhabditis elegans.

Histone lysine methylation can be removed by JmjC domain-containing proteins in a sequence- and methylation-state-specific manner. However, how substrate specificity is determined and how the enzymes are regulated were largely unknown. We recently found that ceKDM7A, a PHD- and JmjC domain-containing protein, is a histone demethylase specific for H3K9me2 and H3K27me2, and the PHD finger binding to H3K4me3 guides the demethylation activity in vivo.

[Impedance characteristics of ear acupoints in identifying excess or deficiency syndrome of stroke].

Objective: To explore the impedance characteristics of ear acupoints in stroke patients with excess or deficiency syndrome, and to provide basis data for objective study of the syndromes of stroke.

Methods: The data of electrical characteristics of ear acupoints in stroke patients and healthy people were collected, and excess syndrome and deficiency syndrome of stroke were identified by quantifying the syndromes of stroke using scales. The differences in impedance characteristics of ear acupoints between stroke patients and healthy people were analyzed, and the differences in impedance characteristics of ear acupoints between stroke patients with excess syndrome and stroke patients with deficiency syndrome were analyzed too.

PHF8 is a histone H3K9me2 demethylase regulating rRNA synthesis.

Dimethylation of histone H3 lysine 9 (H3K9me2) is an important epigenetic mark associated with transcription repression. Here, we identified PHF8, a JmjC-domain-containing protein, as a histone demethylase specific for this repressing mark. Recombinant full-length wild type protein could remove methylation from H3K9me2, but mutation of a conserved histidine to alanine H247A abolished the demethylase activity.

[The effect of short hairpin RNA and DNAzyme gene therapy on latent membrane protein-1 expression in nasopharyngeal carcinoma].

Objective: To study the inhibitory effects of short hairpin RNA (pshLMP1) combined with DNAzyme or alone on latent membrane protein-1 (LMP1)expression in HNE1 cell lines.

Method: The pshLMP1 and DNAzymes were co-transfected with pEGFP-N1-1158 (a expression vector encoding LMP1 and EGFP fusion protein) into HNE1 cells, divided into 4 groups as positive control, RNA interference, combining and DNAzyme groups. The green fluorescence were analyzed and then the mRNA and protein of LMP1 gene were detected.

[Effects of costimulatory molecule VSIG4 exclusively expression in macrophage on renal interstitial fibrosis].

Aim: To investigate the relationship between costimulatory molecule VSIG4 expression in macrophage and mice renal interstitial fibrosis.

Methods: A total of 40 healthy male C57B6 mice (VSIG4(-/-); and VSIG4(+/+);) were divided into two groups, VSIG4(-/-); tubulointerstitial Nephritis (n=20) and VSIG4(+/+); tubulointerstitial Nephritis (n=20), mice received left urethral obstruction operation(UUO) then were killed at 7 d before operation, 3 d, 7 d and 14 d post operation for both groups.Blood SCr and BUN were detected.

Endoscopic resection of small gastrointestinal stromal tumors.

Background: The common treatment principle of gastrointestinal stromal tumors (GISTs) is complete resection of the tumor. The rapid development of endoscopic skill makes it possible to resect GISTs en-bloc through the endoscopic approach.

Aims: The aim of this study is to evaluate the feasibility of endoscopic resection for small-size (<30 mm) gastric GISTs.

Up-regulation of LSB1/GDU3 affects geminivirus infection by activating the salicylic acid pathway.

Geminiviruses include a large number of single-stranded DNA viruses that are emerging as useful tools to dissect many fundamental processes in plant hosts. However, there have been no reports yet regarding the genetic dissection of the geminivirus-plant interaction. Here, a high-throughput approach was developed to screen Arabidopsis activation-tagged mutants which are resistant to geminivirus Beet severe curly top virus (BSCTV) infection.

[Effects and safety of gonadotrophin-releasing hormone agonist combined with estradiol patch and oral medroxyprogesterone acetate on endometriosis].

Objective: To evaluate effects and safety of gonadotrophin-releasing hormone agonist (GnRH-a) combined with transdermal estradiol and medroxyprogesterone acetate in the treatment of endometriosis.

Methods: From January 1st, 2007 to July 31st, 2007, 28 endometriosis patients underwent laparoscopic or transabdominal surgery in Obstetrics and Gynecology Hospital affiliated to Fudan University were randomly divided into group A and group B. 14 patients in group A received 3.

The redox switch: dynamic regulation of protein function by cysteine modifications.

Reactive oxygen intermediates (ROIs) and reactive nitrogen intermediates (RNIs) have now become well established as important signalling molecules in physiological settings within microorganisms, mammals and plants. These intermediates are routinely synthesised in a highly controlled and transient fashion by NADPH-dependent enzymes, which constitute key regulators of redox signalling. Mild oxidants such as hydrogen peroxide (H(2)O(2)) and especially nitric oxide (NO) signal through chemical reactions with specific atoms of target proteins that result in covalent protein modifications.

[Study on the objectivity of traditional Chinese medicinal tongue inspection].

The traditional Chinese medicinal (TCM) inspection on tongue features are always observed with naked eye, and the syndrome is differentiated depended only on experience. These disadvantages cannot but badly impact not only the inheritance but also the advance and development of TCM. Hence, to bring out the standardization and objectivity of TCM tongue inspection is a task urgently expected.

Characteristics of expanded CD4+CD28null T cells in patients with chronic hepatitis B.

Expanded CD4(+)CD28(null) T cells have not been described in the circulation of patients with chronic hepatitis B (CHB). The aim of the present was to detect and characterize the surface phenotype and functional capacity of these cells in CHB patients. Expanded CD4(+)CD28(null) T cells were detected in the circulation of CHB patients with high viral load and elevated aminotransferase levels.

DNA vaccine against tumor endothelial marker 8 inhibits tumor angiogenesis and growth.

Numerous evidences indicated that angiogenesis was a crucial step in the development of tumor, thus the immunotherapy of antiangiogenesis has been involved as potentially relevant candidate targets of cancer immunotherapy. Recently, tumor endothelial marker 8 (TEM8) was discovered to be dominantly expressed in tumor endothelium, which presented a new strategy to antiangiogenesis therapy. However, up to date, the related study of TEM8 on antiangiogenesis is very few.

[Plant SNAREs and their biological functions].

The signal communication between various organelles is essential for cells of eukaryotic organisms. Vesicle trafficking is an important pathway for this kind of communication. Most of the membrane fusion is mediated by SNAREs (Soluble N-ethyl-maleimide-sensitive fusion protein attachment protein receptors), which are highly conserved from various species.