Core N-DRC components play a crucial role in embryonic development and postnatal organ development.

Motile cilia and flagella are evolutionarily conserved organelles, and their defects cause primary ciliary dyskinesia (PCD), a disorder characterized by systemic organ dysfunction. The nexin-dynein regulatory complex (N-DRC) is a crucial structural component of motile cilia and flagella, present across various species from Chlamydomonas to humans. Defects in N-DRC components lead to multiple PCD symptoms, including sinusitis and male infertility.

Hydrogen sulfide attenuates PM2.5-induced COPD by inhibiting cellular senescence via the Klotho/Parkin-mediated mitophagy signaling pathway.

Fine particulate matter (PM2.5), an atmospheric pollutant, plays a crucial role in mediating the occurrence of chronic obstructive pulmonary disease (COPD). Hydrogen sulfide (HS) has shown therapeutic potential in countering COPD, but the underlying mechanisms remain elusive.

Lymphocyte/monocyte to lactate dehydrogenase ratio prior to lymphodepletion impact the outcomes of patients with diffused large B cell lymphoma undergoing CAR-T cell therapy.

Factors associated with outcomes of chimeric antigen receptor (CAR)-T cell therapy in patients with relapsed or refractory (R/R) diffuse large B-cell lymphoma (DLBCL) have not been fully elucidated. We explored the impact of the prelymphodepletion (pre-LD) lymphocyte to monocyte ratio (LMR) and its ratio to lactate dehydrogenase (LDH) (LMR/LDH) on the efficacy and prognosis of 60 patients with R/R DLBCL undergoing CAR-T cell therapy. The optimal cutoff values for pre-LD LMR and LMR/LDH were 3.

Transcription factor VIB-1 activates catalase-3 expression by promoting PIC assembly in Neurospora crassa.

The "p53-like" superfamily transcription factor, VIB-1, plays a crucial role in mediating heterokaryon incompatibility and regulating the transcription of specific genes involved in the secretion of extracellular hydrolases in Neurospora crassa. However, the precise mechanism underlying the transcriptional regulatory function of VIB-1 is still poorly understood. Here, we reveal that VIB-1 is involved in the H2O2-induced oxidative stress response, in which deletion of vib-1 leads to an H2O2-sensitive phenotype and inhibition of cat-3 expression.

A single-dose, four-cycle, fully repetitive crossover bioequivalence of dabigatran etexilate in Chinese.

Purpose: Among healthy Chinese subjects, two capsules of dabigatran etexilate were tested for bioequivalence.

Method: Fifty healthy subjects were recruited for each of the fasting and postprandial trials in a randomized, two-sequence, open-label, four-cycle, fully replicated trial design of a single 150 mg dose of either the test or the reference formulation of dabigatran etexilate capsules in the fasting and postprandial states. The blood concentration of dabigatran at different time points after administration was determined by high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS).

NLRP4 unlocks an NK/macrophages-centered ecosystem to suppress non-small cell lung cancer.

Background: Tumor immune evasion extends beyond T cells, affecting innate immune elements like natural killer cells (NK) and macrophages within the tumor-immune microenvironment (TIME). Nevertheless, translational strategies to trigger collaboration of NK cells and macrophages to initiate sufficient anti-tumor cytoxicity remain scarce and are urgently needed.

Methods: In this study, TCGA datasets was used to confirm the prognosis value of the expression level of NLR family pyrin domain containing 4 (NLRP4) in NSCLC and the tumor tissues microarray was used to further check its clinical-relevance at protein-level.

Adverse event profile of crizotinib in real-world from the FAERS database: a 12-year pharmacovigilance study.

Aim: Crizotinib, an anaplastic lymphoma kinase tyrosine kinase inhibitor (ALK-TKI). It gained approval from the U.S.

Lactate levels and the modified age-adjusted quick sequential organ failure assessment (qSOFA) score are fair predictors of mortality in critically ill pediatric patients.

Introduction: This study aimed to evaluate the predictive accuracy of baseline lactate levels and the modified age-adjusted quick Sequential Organ Failure Assessment (qSOFA) score in forecasting mortality in critically ill pediatric patients.

Methods: A retrospective single-center analysis was conducted on patients aged ≤18 years admitted to the intensive care unit (ICU) between January 1, 2019, and December 31, 2023. The predictive performance of lactate levels at baseline and that of the modified age-adjusted qSOFA score were compared in terms of mortality.

Multi-omics analyses of the gut microbiota and metabolites in children with metabolic dysfunction-associated steatotic liver disease.

The development and severity of metabolic dysfunction-associated steatotic liver disease (MASLD) in children are closely related to alterations of gut microbiota. This study aims to investigate changes in the gut microbiota signature and microbial metabolites in children with MASLD. We collected fecal samples from children and adolescents aged 6-16 years, and the presence of MASLD was diagnosed by ultrasound.

Erratum: Astrocytic YAP protects the optic nerve and retina in an experimental autoimmune encephalomyelitis model through TGF-β signaling: Erratum.

[This corrects the article DOI: 10.7150/thno.60031.

Complete androgen insensitivity syndrome in twins with discordant phenotypes: a case report and review of the literature.

Background: Complete androgen insensitivity syndrome is caused by inactivated mutations in the androgen receptor gene, which results in complete androgen resistance and a female phenotype with a 46,XY karyotype. This condition is rare in twins.

Case Presentation: We report on a Han Chinese twin girl, aged 18 years, with the presence of a vagina and breasts but no uterus and ovaries and chromosomal karyotype analysis showing 46, XY, who was diagnosed with complete androgen insensitivity syndrome.

SP7 transcription factor ameliorates bone defect healing in low-density lipoprotein receptor-related protein 5 (LRP5)-dependent osteoporosis mice.

Loss-of-function variants of low-density lipoprotein receptor-related protein 5 (LRP5) can lead to reduced bone formation, culminating in diminished bone mass. Our previous study reported transcription factor osterix (SP7)‍-binding sites on the promoter and its pivotal role in upregulating LRP5 expression during implant osseointegration. However, the potential role of SP7 in ameliorating LRP5-dependent osteoporosis remained unknown.

Protection conferred by mucosal novel bivalent Klebsiella pneumoniae vaccine immunization associates with presence of lung CD4 T.

Klebsiella pneumoniae is the principal cause of hospital-acquired infection with a high morbidity and mortality in immunocompromised individuals, yet no vaccine is approved. Here, we developed a novel bivalent subunit vaccine for the prevention of K. pneumoniae infection based on the outer membrane protein GlnH and the fimbriae protein FimA.

Chelation treatment for heavy metals in municipal solid waste incineration fly ash: 300-Day study on stability and environmental risk.

Landfilling after chelation is the primary method for treating municipal solid waste incineration fly ash (MSWI FA), but the long-term environmental risks of its heavy metals (HMs) remain unclear. This study used three common organic chelating agents (CAs) to stabilize MSWI FA and assessed its 300-day stability under mixed and zoned landfill scenarios. The findings showed that Pb leaching posed high environmental risks, especially in zoned landfills, with 10-year cumulative leaching of 300 mg/kg, while Cu and Zn leaching remained within limits, indicating lower environmental risks.

Association of HIV infection, other sexually transmitted infections or their coexistence with mpox among men who have sex with men: A national questionnaire-based study in China using propensity score matching.

Background: A marked increase in mpox cases was observed in May 2022 in previously non-endemic regions. The men who have sex with men (MSM) population exhibited a disproportionately higher rate of infection.

Objectives: This study aimed to investigate the coexistence of HIV infection and other sexually transmitted infections (STIs) and their association with mpox among MSM, with validatiton conducted through a case-control study utilizing propensity score matching (PSM).

DHS regulates leaf senescence by modulating gene translation in .

The biochemical and transcriptional regulatory mechanisms of chlorophyll metabolism have been extensively studied, but the translational regulatory mechanisms remain poorly understood. In this study, we found that Nt DHS1 deficiency in N. tabacum resulted in smaller leaves and increased leaf chlorophyll content.

Mutational Landscape of Recurrent/Metastatic Head and Neck Squamous Cell Carcinoma and Association with Immune Checkpoint Inhibitor Outcome.

Purpose: Understanding the mutational landscape of recurrent/metastatic head and neck squamous cell carcinoma (R/M HNSCC) is important in identifying biomarkers to determine which patients may benefit from immune checkpoint inhibitors (ICIs).

Patients And Methods: The HAWK (NCT02207530), CONDOR (NCT02319044), and EAGLE (NCT02369874) studies evaluated R/M HNSCC treatment with durvalumab or durvalumab-tremelimumab. Tumor tissue samples pooled from HAWK/CONDOR (n=153) and plasma cell-free DNA samples from EAGLE (n=285) were analyzed to identify somatic alterations and association with survival.

Genomic surveillance of emerging SARS-CoV-2 Omicron variations in Tianjin Municipality, China 2022.

The coronavirus disease 2019 (COVID-19) pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has severely impacted public health. In 2022, the Omicron variant of SARS-CoV-2 rapidly became the dominant circulating variant in the local COVID-19 outbreaks in Tianjin Municipality, China. To gain a deeper understanding of the genetic variations of the Omicron variant in Tianjin, specimens from individuals who tested positive for SARS-CoV-2 between December 2021 and November 2022 were used for virus whole genome sequencing and phylogenetic analysis.

Spatial Gene Expression of Human Coronary Arteries Revealed the Molecular Features of Diffuse Intimal Thickening in Explanted Hearts.

Diffuse intimal thickening (DIT) is a pre-clinical stage of atherosclerosis characterized by thickened intima. The molecular basis of its susceptibility to atherogenesis is unknown, and mechanistic investigations cannot be performed in commonly used mouse models, in which DIT does not exist. Vascular smooth muscle cells (SMCs) are the predominant cell type that occupies the intima and media of DIT.

A Network Pharmacology Study and In Vitro Evaluation of the Bioactive Compounds of Leaf Extract for the Treatment of Type 2 Diabetes Mellitus.

is a traditional Chinese medicine whose roots have long been used to treat various ailments, but little is known about the efficacy of its leaves. In this study, the antidiabetic activity of leaf extract (KCLE) was determined, the main components of KCLE were identified using UPLC-TOF-MS, and network pharmacology and molecular docking were integrated to elucidate the antidiabetic mechanism of KCLE. The results showed that KCLE effectively increased the glucose consumption of IR-HepG2 cells through pyruvate kinase (PK) and hexokinase (HK), promoted glycogen synthesis, and inhibited α-glucosidase and α-amylase activities.

Co-decoding dynamics in volatiles and sensory profiles of Zhaiji millet vinegar during aging by integrating multiple flavor characterization techniques and chemometrics.

Zhaiji millet vinegar (ZJMV) is a signature acidic condiment. To investigate the effect of aging on the flavor of ZJMV, here gas chromatography-mass spectrometry (GC-MS), gas chromatography-ion mobility spectrometry (GC-IMS), electronic nose, electronic tongue, and quantitative descriptive analysis were used to characterize the flavor profiles of ZJMV for five aging years (2 to 10 years). GS-MS and GC-IMS identified 84 and 44 volatile organic compounds (VOCs), with the most abundant alcohols and esters (p < 0.

Frequent Hand Hygiene-Induced Skin Symptoms and Alterations in Hand Microbiota: A Neglected Form of Chronic Occupational Exposure Among Health Care Workers.

Background: Frequent hand hygiene is essential for infection control among health care workers (HCWs) but may cause adverse skin effects.

Purpose: To assess the relationships between frequent hand hygiene practices, skin symptoms, and microbiota alterations in HCWs.

Methods: A comprehensive search of 7 databases was conducted to identify articles published between January 2014 and July 2024 in English and Chinese.

Exploring the standardization of human nasal antibody measurements.

Mucosal immunity is crucial for preventing the infection and transmission of respiratory viruses. Nasal antibody is inversely correlated with a lower risk of infection with respiratory viruses. However, the current reference standard for nasal antibody assessment is serum-based, mainly consisting of monomeric IgG and IgA.

Exercise addiction in college students: the impact of body dissatisfaction, stress, physical activity and gender.

Introduction: Engaging in physical activity is commonly regarded as beneficial to health. However, exercise addiction may arise when enthusiasm for exercise reaches a level that disrupts life balance and overall well-being. Factors influencing college students' exercise addiction remain largely unknown.