Publications by authors named "Wang Xiudan"

The recent article by Zhang et al. piqued the interest. An atom-modification-based strategy is reported to enhance the cleavage activity of TtAgo, improving its practicability in TtAgo-based nucleic acid testing.

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Single-domain antibodies (sdAbs) often exhibit superior thermal stability compared to traditional antibodies. Efforts are currently focused on enhancing their structural robustness and thermal refolding ability through protein engineering to achieve greater thermal properties and functionality in practical applications. Thermal aggregation is a key factor hindering the reversible thermal denaturation of sdAbs.

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The complex and dynamic nature of aquatic ecosystems, particularly in marine environments, makes managing wound infections a significant challenge for individuals engaged in underwater activities and for aquatic organisms. Although antibiotics have played a critical role in safeguarding humans and aquatic health, their risk of drug resistance and environmental impact present substantial obstacles to long-term sustainability. Using fin rot disease in turbot (Scophthalmus maximus) caused by infection of Vibrio anguillarum (V.

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Timely and accurate assessment of the freshness of aquatic products with complex matrices remains significant challenge. In this study, we developed a dual-mode detection method for hypoxanthine (Hx) based on dual-decomposition mechanisms and simple catalytic reactions. The colorimetric mode, requiring 15 min, is ideal for on-site assessments, while the ratiometric fluorescence mode, equipped with a built-in reference signal, effectively reduces interference from complex matrices.

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Traditional antibody preparation methods rely on animal immunization and experimental screening, often requiring 3-6 months, which cannot meet the urgent demands for antibodies in environmental emergencies or contamination events. Herein, a rapid antibody preparation method was proposed to transform anti-macromolecule nanobodies into high-affinity anti-small molecule (enrofloxacin) nanobodies, based on the high-precision predictive capabilities of AlphaFold, integrated with structural and interaction analysis. The high-precision prediction capability of AlphaFold ensured the accuracy of nanobody structural analysis and targeted modification.

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Massive blood loss is the main cause of prehospital trauma-related death, the development of rapid and effective hemostatic materials is imminent. Injectable hydrogels have the advantages of covering irregular bleeding sites and quickly closing the wound. However, its inherent viscosity can easily precipitate tissue adhesion and other complications.

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This study aimed to explore the relationship between hypoxanthine (Hx) levels and freshness indicators in three kinds of fish samples during storage for 7 days at 4 °C to determine the Hx levels indicating freshness. The total volatile basic nitrogen (TVBN) was tested using the semi-micro Kjeldahl method, and Hx level and K value were measured using high-performance liquid chromatography (HPLC). During storage, Hx levels, TVBN, and K values increased, and distinct patterns were observed between Hx and TVBN in three fish samples.

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Insulin resistance and pancreatic β-cell dysfunction are the main pathogenesis of type 2 diabetes mellitus (T2DM). However, insulin therapy and diabetes medications do not effectively solve the two problems simultaneously. In this study, a biomimetic oral hydrogen nanogenerator that leverages the benefits of edible plant-derived exosomes and hydrogen therapy was constructed to overcome this dilemma by modulating gut microbiota and ameliorating oxidative stress and inflammatory responses.

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Dryocosmus kuriphilus, commonly known as the chestnut gall wasp, belongs to the family Cynipidae and is native to China. It is a highly invasive insect species causing serious damage to chestnut trees and has rapidly spread to various continents, including Europe, North America, and Oceania. The D.

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Background: Due to the serious issue of ofloxacin (OFL) abuse, there is an increasingly urgent need for accurate and rapid detection of OFL. Immunoassay has become the "golden method" for detecting OFL in complex matrix beneficial to its applicability for a large-scale screening, rapidity, and simplicity. However, traditional antibodies used in immunoassay present challenges such as time-consuming preparation, unstable sensitivity and specificity, and difficulty in directional evolution.

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Intelligent food packaging has recently gained significant attention due to the heightened consumer awareness regarding food quality. Although anthocyanins avoid safety issues, the instability and leakage of anthocyanins restrict their utilization in freshness indicator labels. In this study, we introduced an innovative metal-organic framework (UiO-66-NH) synergistic pH-colorimetric label with fast ammonia-responsive, incorporating sodium alginate, red cabbage anthocyanin, and UiO-66-NH.

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is a pest that severely affects oil tea plants, and the Notch signaling pathway plays a significant role in the early development of insect ovaries. In this study, we explored the function of the gene within the Notch signaling pathway in the reproductive system of . The functional domains and expression patterns of were analyzed.

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Hypoxanthine (Hx), produced by adenosine triphosphate (ATP) metabolism, is a valuable indicator that determines the quality and degradation status of meat products and is also an important biochemical marker to certain diseases such as gout. The rapid emergence of paper-based enzyme biosensors has already revolutionized its on-site determination. But it is still limited by the complex patterning and fabrication, unstable enzyme and uneven coloration.

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Purpose: This study aimed to investigate the composition of ocular surface microbiota in patients with obesity.

Methods: This case-control study, spanning from November 2020 to March 2021 at Henan Provincial People's Hospital, involved 35 patients with obesity and an equivalent number of age and gender-matched healthy controls. By employing 16S rRNA sequencing, this study analyzed the differences in ocular surface microbiota between the two groups.

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Article Synopsis
  • Massive blood loss from injuries is the top cause of prehospital deaths in emergencies, highlighting the need for effective hemostatic materials to quickly stop bleeding and save lives.
  • Researchers have created various hemostatic materials, but these need to offer additional functions for specific use cases, such as positioning in wounds, withstanding pressure, and being easy to remove after treatment.
  • This review discusses challenges in using these materials and the advancements researchers are making to improve them, aiming to guide the future design of more effective and adaptable options.
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Article Synopsis
  • The text indicates that there is a correction made to a specific article, identified by its DOI number (10.1016/j.apsb.2022.04.018).
  • The correction likely addresses errors or inaccuracies found in the original publication.
  • This implies the importance of maintaining accurate scientific literature for the benefit of the research community.
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A new species of gall inquiline, , is described from Hubei Province, China. Morphological descriptions, photographs and biological information are provided. Mitochondrial cytochrome oxidase (COI) sequences of the new species were newly obtained and a molecular species delimitation analysis of 12 species of performed using the ASAP method recovered 16 molecular operational taxonomic units, providing support for recognition of the new species.

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Article Synopsis
  • Uncontrollable bleeding from deep wounds is a major problem in war and disaster situations, leading to many deaths.
  • Injectable hydrogels are being studied as a promising solution for managing this type of hemorrhage, with research focusing on their properties and mechanisms.
  • The review discusses the pros and cons of these hydrogels, current research progress, and future possibilities for improving their effectiveness, including enhancing biocompatibility and promoting better wound healing.
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Ventilator-induced lung injury (VILI) disturbs the disordered immune system and causes persistent inflammatory damage. 4-octyl itaconate (OI) is a synthetic cell-permeable itaconate derivative with antioxidant and anti-inflammatory effects. In this study, we assessed whether OI protects against VILI.

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Background: Modifications in the gut microbiota may be a crucial factor in the efficacy of canagliflozin (Cana) in managing patients with type 2 diabetes mellitus (T2DM). However, the interplay between oral and ocular surface microbiota and this treatment remains poorly explored.

Aim: This study aimed to assess alterations in the gut, oral, and ocular surface microbiota pre- and post-Cana treatment in patients with T2DM.

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Small-diameter vascular grafts (SDVGs) are urgently required for clinical applications. Constructing vascular grafts mimicking the defining features of native arteries is a promising strategy. Here, we constructed a tri-layered vascular graft with a native artery decellularized extracellular matrix (dECM) mimicking the component of arteries.

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The skin is the body's first line of defence, and its physiology is complex. When injury occurs, the skin goes through a complex recovery process, and there is the risk of developing a chronic wound. Therefore, proper wound care is critical during the healing process.

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This study aimed to analyze the effect of methylglyoxal (MGO) on the structure and allergenicity of shrimp tropomyosin (TM) during thermal processing. The structural changes were determined by SDS-PAGE, intrinsic fluorescence, circular dichroism, and HPLC-MS/MS. The allergenicity was evaluated by and experiments.

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Marine organisms need to adapt to improve organismal fitness under ocean acidification (OA). Recent studies have shown that marine calcifiers can achieve acclimation by stimulating calcium binding/signaling pathways. Here, a CaM-like gene (CgCaLP-2) from oyster Crassostrea gigas which typically responded to long-term CO exposure (two months) rather than short-term exposure (one week) was characterized.

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Glycolysis plays a vital role in the development and progression of tumors. Inhibiting glycolysis via smart and safe methods serves as a promising target for cancer therapy. Here, an oral "sugar-coated bullet" aiming at intervening Warburg effect is designed by coating colloidal mesoporous silica nanoparticles (CMS) encapsulating glycolysis inhibitor shikonin (SHK) with dextran, namely DCMS/SHK.

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